MATHEMATICAL MODELS FOR THE SYNTHESIS OF PLANT-BASED COMPOSITIONS WITH IMPROVED AMINO ACID COMPOSITION

I. Gaivoronskaya, V. Kolpakova
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Abstract

The aim of the work was to optimize the process of obtaining multicomponent protein compositions with high biological value and higher functional properties than the original vegetable protein products. Was realized studies to obtain biocomposites on the base of pea protein-oat protein and pea protein-rice protein. Developed composites were enriched with all limited amino acids. For each of the essential amino acids, the amino acid score was 100% and higher. Protein products used in these compositions are not in major allergen list, which allows to use these compositions in allergen-free products and specialized nutrition. To determine biosynthesis parameters for compositions from pea protein and various protein concentrates with the use of transglutaminase enzyme, was studied effect of concentration and exposition time on the amount of amino nitrogen released during the reaction. Decreasing of amino nitrogen in the medium indicated the occurrence of a protein synthesis reaction with the formation of new covalent bonds. Were determined optimal parameters of reaction: the hydromodule, the exposure time, the concentration of EP of the preparation, were obtained mathematical models. Studies on the functional properties of composites, the physicochemical properties of the proteins that make up their composition, and structural features will make it possible to determine the uses in the manufacture of food products based on their ability to bind fat, water, form foam, gels, and etc.
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改进氨基酸组成的植物基组合物合成的数学模型
本工作的目的是优化工艺,获得比原植物蛋白产品具有高生物价值和更高功能特性的多组分蛋白质组合物。实现了以豌豆蛋白-燕麦蛋白和豌豆蛋白-大米蛋白为基础制备生物复合材料的研究。开发的复合材料富含所有限制性氨基酸。对每一种必需氨基酸,氨基酸得分均在100%以上。这些组合物中使用的蛋白质产品不在主要过敏原清单中,这使得这些组合物可以用于无过敏原产品和专门的营养。为确定转谷氨酰胺酶对豌豆蛋白和各种蛋白浓缩物的生物合成参数,研究了浓度和暴露时间对反应过程中氨基氮释放量的影响。培养基中氨基氮的减少表明发生了蛋白质合成反应,形成了新的共价键。确定了反应的最佳参数:水模、曝光时间、制备的EP浓度,并建立了数学模型。对复合材料的功能特性、组成复合材料的蛋白质的物理化学特性和结构特征的研究,将有可能根据复合材料结合脂肪、水、形成泡沫、凝胶等的能力,确定其在食品制造中的用途。
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