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CAPILLARY RISE CHARACTERISTICS AND SALTWATER PROPAGATION IN FINE AGGREGATE: TOWARD DEVELOPING THE ANTI-SALINITY SHALLOW FOUNDATION 细骨料中毛细上升特性与盐水传播——浅谈抗盐浅基础的开发
Pub Date : 2021-10-28 DOI: 10.32008/geolinks2021/b1/v3/46
N. Truc, N. Hoang, T. Tu
This study is carried out to determine the capillary rise over time of fine aggregate in different saline media. The obtained results showed that the capillary height in fine aggregate is inversely proportional to the salt concentration of the capillary solution. The aggregate that has a particle size over 2.0 mm shows the best ability to limit capillary rise. The capillary height of the aggregate gets the highest value when there is no salt in the solution and gets the lowest one with the solution at the highest salinity tested, i.e. 33.0 g/L. The obtained results on capillary characteristics lead to an idea of design a shallow foundation that has a function of anti-corrosion, anti-salinity proactively and effectively.
本研究是为了确定细骨料在不同的盐水介质中随时间的毛细管上升。结果表明,细集料中毛细高度与毛细溶液的盐浓度成反比。粒径大于2.0 mm的骨料抑制毛细上升的能力最好。当溶液中不含盐时,骨料的毛细高度达到最大值,当溶液含盐量最高(33.0 g/L)时,骨料的毛细高度达到最小。通过对毛细管特性的研究,提出了主动有效地设计具有防腐、防盐功能的浅基础的思路。
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引用次数: 1
OBTAINING NUCLEIC ACID PREPARATIONS AND THEIR HYDROLYSATES FROM BIOMASS OF METHANE-OXIDIZING BACTERIA 从甲烷氧化菌生物量中获得核酸制剂及其水解物
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b1/v3/14
A. Krasnoshtanova, E. Borovkova
"Due to the unfavourable environmental, social and economic situation, the need for the treatment of oncological diseases and diseases associated with impaired activity of the immune system is increasing. A lot of these drugs are made on the basis of nucleic acid components, the industrial production of which is practically non-existent in Russia. Therefore, a task of current interest is to develop the basis of the technology for obtaining components of nucleic acids, which can be widely used in medicine as immunomodulatory, wound-healing, antiviral, and diagnostic medicine, as well as for cancer treatment. Most of the described in literature methods of isolating nucleic acid components from plant, animal and microbial raw materials are based on the use of toxic and expensive organic solvents, that’s why it is impossible to apply these methods outside of laboratory conditions. The most promising source of raw materials for nucleic acids is the biomass of microorganisms (yeast and bacteria) from biomass, since the use of such source makes it possible to quickly obtain a large enough amount of biomass, and, consequently, a larger amount of nucleic acids. This allows obtaining DNA in addition to RNA. RNA and DNA substances can be used to obtain nucleosides and nitrogenous bases, which are also widely used in medicine. The purpose of these studies was to select the conditions for the extraction of RNA and DNA from the biomass of methane-oxidizing bacteria in one technological cycle, as well as to compare the efficiency of alkaline and acid hydrolysis of microbial RNA and DNA. The need for a two-stage extraction of nucleic acids from the biomass of methane-oxidizing bacteria in order to separately extract RNA and DNA was Substantiated. It was ascertained that at the first stage of extraction at a temperature of 90 ° C, pH 9.0 for 90 min, at least 85% of RNA is extracted. After the separation of the extract by centrifugation, the partially denuclearized biomass must be re-processed under the same conditions in order to extract DNA by at least 83%. The modes of concentration of RNA and DNA solutions by ultrafiltration were selected. It was found that in order to achieve effective deposition of nucleic acids at the isoelectric point, the concentration of the RNA solution must be carried out on the UPM-10 membrane at the concentration degree of 7, and the DNA solution on the UPM-100 membrane at the concentration degree 6. The dynamics of decomposition of nucleic-protein complexes in the medium of monoammonium phosphate was investigated. It was shown that the transition of NA into solution by at least 80% is achieved at a monoammonium phosphate concentration of 1.7 M, a temperature of 55 ° C for 90 min. The use of 5-fold washing of oligonucleotide substances with acidified water (pH 2.0) to remove excess mineral impurities was substantiated. А comparative assessment of acid and alkaline hydrolysis of RNA and DNA was carried out in order to obtain derivativ
“由于不利的环境、社会和经济状况,对肿瘤疾病和与免疫系统活动受损有关的疾病的治疗需求正在增加。这些药物中有很多是由核酸成分制成的,而这种成分在俄罗斯几乎不存在工业化生产。因此,当前的一个重要任务是开发核酸成分提取技术的基础,使其广泛应用于免疫调节、伤口愈合、抗病毒、诊断医学以及癌症治疗等医学领域。文献中所描述的从植物、动物和微生物原料中分离核酸组分的方法大多是基于使用有毒且昂贵的有机溶剂,这就是为什么这些方法不可能在实验室条件之外应用。最有希望的核酸原料来源是来自生物质的微生物(酵母和细菌)的生物质,因为使用这种来源可以快速获得足够多的生物质,从而获得更大量的核酸。这使得除了RNA之外,还可以获得DNA。RNA和DNA物质可以用来获得核苷和含氮碱基,在医学上也有广泛的应用。本研究的目的是选择在一个工艺周期内从甲烷氧化菌生物量中提取RNA和DNA的条件,并比较微生物RNA和DNA的碱性和酸性水解效率。证实了从甲烷氧化菌生物量中提取核酸以分别提取RNA和DNA的必要性。结果表明,在90℃,pH 9.0, 90 min的条件下,第一步提取,至少85%的RNA被提取。在离心分离萃取物后,部分去核的生物质必须在相同条件下重新处理,以提取至少83%的DNA。选择了RNA和DNA溶液的超滤浓缩方式。研究发现,为了在等电点实现核酸的有效沉积,RNA溶液必须在浓度度为7的UPM-10膜上进行浓缩,DNA溶液在浓度度为6的UPM-100膜上进行浓缩。研究了磷酸一铵介质中核蛋白复合物的分解动力学。结果表明,在磷酸一铵浓度为1.7 M、温度为55℃、时间为90 min的条件下,NA向溶液的转变率至少达到80%。用酸化水(pH 2.0)对寡核苷酸物质进行5次洗涤,以去除多余的矿物杂质。А为了获得核酸衍生物,我们对RNA和DNA的酸和碱水解进行了比较评估。”
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引用次数: 0
RESEARCH ON THE BEHAVIOR OF AN ASSORTMENT OF BELL PEPPER ON THE SANDY SOILS IN SOUTH-WEST OLTENIA ACCORDING TO THE CULTIVATION METHOD USED 不同栽培方法对西南甜椒品种在沙质土壤上表现的影响研究
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b1/v3/40
A. Paraschiv, M. Dima, A. Diaconu, E. Ciuciuc, M. Croitoru
The pedo-climatic conditions specific to sandy soils in southwest Oltenia determine differences in performing different phenophases of growth and development of plants, both between varieties and in the physiological behavior of the same variety under different yield conditions. Therefore, at the Dăbuleni Research and Development Station for Plant Culture on Sands, research was carried out on some physiological, biochemical and production processes at five varieties of bell peppers cultivated in the open fields and solar. Determinations of photosynthesis, foliar transpiration, the biochemical composition of fruits, quantity, and quality of bell pepper production were performed. The physiological and biochemical processes studied were influenced by the environmental conditions specific to each cultivation method, but also by the studied variables. Of the 5 varieties studied, the best results on the biochemical composition of the fruits were recorded at the Artim variety (8.83% total dry substance, 4.60% soluble dry substance, 0.19% acidity, 3.80% carbohydrate and 36.96% vitamin C). The production was between 15,387 t / ha for open-field plants and 108,574 t / ha for plants grown in the solar, the differences between the two cultivation methods being statistically assured as distinctly significant.
西南地区沙质土壤特有的土壤气候条件决定了品种间植物生长发育不同物候期的差异,也决定了同一品种在不同产量条件下的生理行为差异。因此,在dbuleni沙地植物栽培研究与开发站,对露天栽培和日光栽培的5个品种甜椒的一些生理生化和生产过程进行了研究。对甜椒的光合作用、叶片蒸腾作用、果实生化成分、产量和品质进行了测定。所研究的生理生化过程不仅受到每种培养方法所特有的环境条件的影响,还受到所研究变量的影响。在5个品种中,果实生化组成的最佳结果是Artim品种(总干物质8.83%,可溶性干物质4.60%,酸度0.19%,碳水化合物3.80%,维生素C 36.96%),产量在露天植株15387 t / ha和太阳能植株108574 t / ha之间,两种栽培方式之间的差异有统计学意义。
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引用次数: 0
REDUCING GREENHOUSE GAS EMISSIONS BY IMPLEMENTING SUSTAINABLE INFRASTRUCTURE PROJECTS 通过实施可持续基础设施项目减少温室气体排放
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b1/v3/02
R. Nicolae, Petru Nicolae, Ana-Maria Brăileanu
Climate change is an inevitable and urgent global challenge with long-term implications for the sustainable development of all countries. In order to respond to climate change, it is very important to identify new ways of reducing greenhouse gas emissions. The present paper emphasizes that the use of a greener and more efficient means of transport, such as a highway (in this case Buzău-Focșani) is more beneficial in terms of reducing greenhouse gas emissions than using old infrastructure. Through specific traffic data processing and dispersion modelling, it was shown that the polluting emissions will increase in the next years, in case of using the already existing road, while the use of a sustainable highway provides a downward trend for these emissions. The construction of the highway will have positive effects on air quality, will ensure a good traffic flow, but the benefits will be even greater when the replacing of the existing car fleet (Diesel vehicles) with hybrid and electric vehicles will take place.
气候变化是一项不可避免的紧迫全球挑战,对所有国家的可持续发展具有长期影响。为了应对气候变化,找到减少温室气体排放的新途径是非常重要的。本论文强调,在减少温室气体排放方面,使用更环保、更有效的交通工具,如高速公路(在本例中为Buzău-Focșani)比使用旧的基础设施更有益。通过具体的交通数据处理和分散建模,表明在使用现有道路的情况下,未来几年的污染排放将增加,而使用可持续公路则提供了这些排放的下降趋势。高速公路的建设将对空气质量产生积极影响,将确保良好的交通流量,但当现有的车队(柴油车)被混合动力和电动汽车取代时,好处将会更大。
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引用次数: 0
MODELING OF DYNAMIC PROCESSES IN THE BLACK SEA AND ATMOSPHERE IN PERSPECTIVE OF THEIR COUPLING FOR THE BLACK SEA REGION 黑海地区黑海和大气动力学过程的耦合模拟
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b2/v3/16
D. Demetrashvili, A. Surmava, V. Kukhalashvili
"At the modern stage of the development of Geosciences, the study of hydrothermodynamic and ecological processes occurring in the natural environment (sea, atmosphere, soil), their monitoring and forecasting become very relevant and are a necessary condition for sustainable development of society. The Caucasus region is one of the most difficult regions of the world from the point of view its physical and geographical features. These features include the Black and Caspian Seas and the complex terrain of the Caucasus. The Seas and the atmosphere are unified hydrodynamic systems, between subsystems of which processes of an exchange of energies, momentum and substances continuously take place. One of the most effective ways to study natural and environmental processes is methods of mathematical modeling, which allows reproducing these processes and phenomena and studying the quantitative contribution of various factors to the development of such processes. The purpose of the paper is to discuss the models of the Black Sea and atmospheric dynamics developed at M. Nodia Institute of Geophysics of I. Javakhishvili Tbilisi State University, and some results of their implementation. The model of the Black Sea dynamics is based on a full system of ocean hydro-thermodynamics equations. Its high-resolution version, which is nested in the basin-scale model of the Black Sea dynamics of Marine Hydrophysical Institute (MHI, Sevastopol), is used to forecast main hydrophysical fields for the easternmost part of the Black Sea. The model of the atmospheric dynamics is based on a full system of atmospheric hydro-thermodynamics equations in hydrostatic approximation written in the terrain-following coordinate system and is realized for the extended territory including the eastern part of the Mediterranean Sea and Black and Caspian seas and for the Caucasus region. These models, after some modification will form the basis of the coupled Black Sea-atmosphere limited-area modeling system."
“在地球科学发展的现代阶段,对自然环境(海洋、大气、土壤)中发生的水热力学和生态过程的研究及其监测和预报变得非常相关,并且是社会可持续发展的必要条件。从其自然和地理特征来看,高加索地区是世界上最困难的地区之一。这些特点包括黑海和里海以及高加索地区复杂的地形。海洋和大气是统一的流体动力系统,在子系统之间,能量、动量和物质的交换过程不断发生。研究自然和环境过程最有效的方法之一是数学建模方法,它可以再现这些过程和现象,并研究各种因素对这些过程发展的定量贡献。本文的目的是讨论在第比利斯国立大学贾瓦希什维利地球物理研究所建立的黑海和大气动力学模型及其实施的一些结果。黑海动力学模型是建立在一整套海洋水热力学方程的基础上的。它的高分辨率版本嵌套在海洋水物理研究所(MHI, Sevastopol)黑海动力学的盆地尺度模型中,用于预测黑海最东部的主要水物理场。大气动力学模型是基于在地形跟踪坐标系中写成的流体静力学近似的大气流体热力学方程的完整系统,该模型适用于包括地中海东部、黑海和里海以及高加索地区在内的扩展领土。这些模式经过一些修改,将构成黑海-大气耦合有限区域模式系统的基础。
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引用次数: 0
CONSIDERATIONS ON SARS-COV-2 DIAGNOSIS IN THE LABORATORY OF UNIVERSITY EMERGENCY CLINICAL HOSPITAL OF CONSTANTA 康斯坦察大学急诊临床医院实验室对sars-cov-2诊断的思考
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b1/v3/07
R. Stoicescu, R. Stoicescu, Codrin Gheorghe, Adina Honcea, I. Bratu
Coronaviruses are members of the Coronaviridae family. They are enveloped, non-segmented, positive-sense, single-stranded RNA viruses. Their genome size is about 30 kilobases (kb) which consist, at the 5’ end, of non-structural open reading frames (ORFs: ORF1a, ORF 1b) which code for 16 non structural proteins, and at the 3’ end the genes which code for four structural proteins including membrane (M), envelope (E), spike (S), and nucleocapsid (N) proteins. Due to the rapid spread of COVID-19, a reliable detection method is needed for patient diagnosis especially in the early stages of the disease. WHO has recommended nucleic acid amplification tests such as real-time reverse transcription-polymerase chain reaction (RT-PCR). The assay detects three SARS-CoV-2 RNA targets: the envelope (E) gene, the nucleocapsid (N) gene and a region of the open reading frame (ORF1) of the RNA-dependent RNA polymerase (RdRp) gene from SARS-CoV-2 virus isolate Wuhan-Hu-1. Our study was made in the first 3 months of the year 2021 using the real-time RT PCR results obtained in the Cellular Biology ward of the University Emergency Clinical Hospital. In our lab we are testing the inpatients from the hospital wards (Neurology, Pediatrics, Surgery, Internal medicine, ICU, Cardiology, etc.); we are also testing the outpatients from Dialysis and Oncology, 2 days prior to their therapy; we also test the health care personnel. The number of tests we performed was: in January 1456, with 399 positive results (27.4%), 33 deaths; in February 1273 tests, 221 positive (17.36%), 16 deaths; in March 1471 tests, 373 positive (25.36%), 37 deceased.
冠状病毒是冠状病毒科的成员。它们是包膜的、无节段的、正义的单链RNA病毒。它们的基因组大小约为30千碱基(kb),在5 '端由非结构开放阅读框(ORF: ORF1a, ORF 1b)组成,编码16种非结构蛋白,在3 '端由编码4种结构蛋白的基因组成,包括膜(M),包膜(E),穗(S)和核衣壳(N)蛋白。由于COVID-19的快速传播,需要一种可靠的检测方法来诊断患者,特别是在疾病的早期阶段。世卫组织建议进行核酸扩增检测,如实时逆转录聚合酶链反应(RT-PCR)。该方法检测SARS-CoV-2病毒武汉湖1号分离株的包膜(E)基因、核衣壳(N)基因和RNA依赖性RNA聚合酶(RdRp)基因开放阅读框(ORF1)区域3个SARS-CoV-2 RNA靶点。我们的研究是在2021年前3个月利用在大学急诊临床医院细胞生物学病房获得的实时RT PCR结果进行的。在我们的实验室里,我们对来自医院病房(神经内科、儿科、外科、内科、ICU、心脏病科等)的住院病人进行测试;我们还对透析和肿瘤科门诊患者在治疗前2天进行检测;我们还对医护人员进行了测试。我们进行的检测次数为:1月1456次,阳性结果399例(27.4%),33例死亡;2月检测1273例,阳性221例(17.36%),死亡16例;3月检测1471例,阳性373例(25.36%),死亡37例。
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引用次数: 0
ECOLOGICAL EDUCATION - ELEMENTS OF THE NATIONAL STRATEGY FOR THE SUSTAINABLE DEVELOPMENT OF ROMANIA 2030 生态教育——罗马尼亚2030年可持续发展国家战略的组成部分
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b2/v3/08
A. Ioana, D. Marcu, Daniela Luta, B. Ene, D. Juganaru
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引用次数: 1
TREE SPECIES DIVERSITY AND SPATIAL DISTRIBUTION OF HOLM OAK STANDS IN CHETTABA FOREST (ALGERIA) 阿尔及利亚切塔巴森林黑栎林分树种多样性及空间分布
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b2/v3/28
Zerrouki Alia, Kara Karima, R. Lilia, R. Malika
The forest of Chettaba is for ecotourism. Managed by the Forest Conservation of Constantine, this site is formed of several forestry species (hardwood and softwood) spread over an area of 3010 ha. The objective of this study is to estimate the viability of this site through the permanent monitoring of the current state of the forest including biodiversity and pressures. The collection of data related to it had recourse to a forest inventory and an inventory of pressures. The condition was assessed by analyzing these components. The various analyses conducted during this study revealed that the forest is subject to biotic and abiotic pressures and average viability. This is induced by a fairly good stand quality (PHF= 333), medium stability (S= 44.45), low mortality (MR = 4.16%) and good future potential (high regeneration rate (RR =231.25%). Grazing, fire and illegal logging are the most important pressures. Despite these pressures, the forest is classified as a viable ecosystem. However, these potentials are insufficient: it is necessary to reduce the pressures and reforest the degraded plots for better conservation of the ecosystem. To this end, the present study suggests management primarily through silvicultural interventions that promote the regeneration of the various species and to include a permanent ecological monitoring system.
切塔巴的森林是用于生态旅游的。该遗址由康斯坦丁森林保护协会管理,由几种森林物种(硬木和软木)组成,分布在3010公顷的面积上。本研究的目的是通过对森林现状(包括生物多样性和压力)的长期监测来估计该地点的生存能力。有关数据的收集依靠森林清单和压力清单。通过分析这些成分来评估条件。在这项研究中进行的各种分析表明,森林受到生物和非生物压力和平均生存能力的影响。这是由于林分质量较好(PHF= 333),稳定性中等(S= 44.45),死亡率低(MR = 4.16%),未来潜力大(再生率高(RR =231.25%)。放牧、火灾和非法伐木是最重要的压力。尽管有这些压力,森林仍被列为一个可行的生态系统。然而,这些潜力是不够的:为了更好地保护生态系统,必须减少压力并在退化的地块上重新造林。为此目的,本研究建议主要通过促进各种物种再生的造林干预措施进行管理,并包括一个永久的生态监测系统。
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引用次数: 0
GLOBAL TRENDS IN BIODEGRADABLE POLYMERS 可生物降解聚合物的全球趋势
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b2/v3/35
I. Usachev, D. Solomin
Recently, the rapid and almost uncontrollable growth in the consumption of synthetic plastics in many sectors of the economy, especially in the field of packaging, has been a serious concern. Plastic containers are used for packaging food products, medicines, electronic devices, liquids, including those with a higher hazard class, etc. [1]. According to the German Nova-Institute, the global plastic production in 2020 has reached almost 400 Mill. Tons. At the same time, the volume of biodegradable plastics obtained from renewable resources amounted to only 3.5 million tons, i.e., about 1% of the total volume production [2]. Considering that only 25% of plastic waste is recycled, the growing consumption of polymer products is forcing manufacturers to develop biodegradable polymer compositions [3]. The problem has economic and environmental aspects since it is interconnected with the growing need to protect the environment and reduce the cost of raw materials for the production of various products.
最近,合成塑料的消费在经济的许多部门,特别是在包装领域的迅速和几乎无法控制的增长,已成为一个严重的问题。塑料容器用于包装食品、药品、电子设备、液体,包括具有较高危害等级的物品等[1]。根据德国nova研究所的数据,到2020年,全球塑料产量将达到近4亿吨。吨。与此同时,从可再生资源中获得的生物降解塑料的产量仅为350万吨,约占总产量的1%[2]。考虑到只有25%的塑料废物被回收利用,聚合物产品消费的不断增长迫使制造商开发可生物降解的聚合物组合物[3]。这个问题有经济和环境方面的问题,因为它与日益增长的保护环境和降低生产各种产品的原材料成本的需要是相互联系的。
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引用次数: 0
THE POSSIBILITY OF USING THE ENERGY POTENTIAL OF WASTE POOL WATER 利用废水池水的能源潜力的可能性
Pub Date : 1900-01-01 DOI: 10.32008/geolinks2021/b1/v3/53
A. Predajnianska, J. Takács
Thermal baths in Slovakia are producers of wastewater, which hides considerable energy potential. The wastewater from the thermal pools has often exceeds the maximum permissible value of the temperature of the wastewater discharged into the water recipient. As a result, there is undesirable damage to the environment, which results in sanctions for the operators of these facilities. Our aim is to present the concept of a single- and double-step heat recuperation system of waste pool water using applications of various types of heat exchangers or heat pumps. The aim of this application is to ensure a suitable temperature to the discharged waste pool water, efficient use of the energy potential of the waste water and saving of primary energy in the form of geothermal water, thus extending the life of the entire system.
斯洛伐克的温泉浴场是废水的生产者,这隐藏了相当大的能源潜力。从热池流出的废水往往超过排放到受水器的废水的最高允许温度值。结果,对环境造成了不希望看到的破坏,导致这些设施的经营者受到制裁。我们的目的是提出利用各种类型的热交换器或热泵应用的单步和双步废水池水热回收系统的概念。该应用程序的目的是确保排放的废水池水具有合适的温度,有效利用废水的能源潜力,并以地热水的形式节省一次能源,从而延长整个系统的使用寿命。
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引用次数: 0
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