Construction and Functional Analysis of Luciferase Reporter Plasmid Containing CAAP1 Gene Promoter

Abstract

Megakaryoblastic leukemia 1 (MKL1) was closely related to the pathogenesis of various human malignant cancers, It has been reported in the literature that MKL-1 could promote the proliferation of gastric cancer cells and affect the cell cycle. And could affect the value-added and migration ability of lung cancer cells.However, The mechanism of MKL-1 affecting autophagy and apoptosis of tumor cells was not clear. Caspase activity and apoptosis inhibitor 1 (CAAP1) was reported to be involved in cell apoptosis, It can participate in the regulation of apoptosis in tumor cells. In this study, human CAAP1 promoter luciferase reporter construct was successfully constructed. Then transfected overexpression vectors of human MKL-1 into 293T cells,and detected the activation of the CAAP1 promoter by luciferase reporter assay. The result showed that transfected the overexpression MKL-1 vectors can enhance the transcriptional activity of CAAP1. Our research will further reveal the apoptosis of gastric cancer cells and may provide theoretical foundations for methods to cure gastric cancer.