Forensic determination of determination of damage to human internal organs by the method of reconstruction of the optical activity of histological sections

Abstract

The article presents the results of experimental testing of the technique of tomographic reconstruction of circular birefringence maps of molecular complexes in a digital histological study of the age of damage to tissues of internal organs (brain, liver and kidney) of temporary monitoring of the mean value, dispersion, asymmetry and kurtosis, characterizing the distribution of the magnitude of the optical activity of histological sections of the brain, liver and kidney identification of the temporal extent of linear changes in statistical parameters and the accuracy of determining the duration of damage to human internal organs by digital histological methods of polarization reconstruction (tomography) of circular birefringence of molecular complexes. Aim of the work. Development of a technique for determining the duration of damage to human internal organs by digital histological methods of polarization reconstruction of circular birefringence of molecular complexes. Materials and methods. The object of the study was the histology of samples of human internal organs (brain, kidney and liver) with different duration of damage from 1 hour to 120 hours. For control, we used BT samples of those who died from coronary artery disease with different duration of damage from 1 hour to 120 hours. The studies were carried out using the technique of polarization reconstruction of circular birefringence of molecular complexes. Results. A set of treatment-relevant relationships between temporal changes in the statistical structure of topographic maps of circular birefringence of optically active molecular complexes of histological sections of human internal organs with different duration of damage and variations in the mean value, dispersion, asymmetry and kurtosis, characterizing the distribution of the value of this parameter of anisotropy, has been determined. Conclusions. A new original method has been developed for tomography of the optical activity of molecular complexes of tissues of human internal organs in a digital histological study of the age of damage to the tissues of the brain, liver and kidney, as well as the myocardium and lung tissue at a time interval of 1 hour. up to 120 hours