Kuan-Chih Lee, Kun-Ting Hsieh, Raymond Chen, W. Lin, Changyuan Wang, Tzu-Tai Lee, Liang-Jwu Chen
{"title":"Expression and Characterization of Rice-produced Recombinant Porcine Lactoferrin and its Antioxidant Activities","authors":"Kuan-Chih Lee, Kun-Ting Hsieh, Raymond Chen, W. Lin, Changyuan Wang, Tzu-Tai Lee, Liang-Jwu Chen","doi":"10.2174/1874070702014010094","DOIUrl":null,"url":null,"abstract":"Lactoferrin (LF) exhibits multiple beneficial biological activities and thus has been used as a health food and additive. To broaden its application in the food industry, the porcine LF (pLF) gene has been engineered into rice to produce recombinant LF (rpLF) for use as a food additive. The ironbinding and antimicrobial activities of rpLF and its positive effects on early weaned piglets have been previously evaluated, yet several features, such as the signal peptide removal, glycosylation sites and antioxidant activity of rpLF, have not been fully characterized.","PeriodicalId":296126,"journal":{"name":"The Open Biotechnology Journal","volume":"145 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Biotechnology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874070702014010094","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Lactoferrin (LF) exhibits multiple beneficial biological activities and thus has been used as a health food and additive. To broaden its application in the food industry, the porcine LF (pLF) gene has been engineered into rice to produce recombinant LF (rpLF) for use as a food additive. The ironbinding and antimicrobial activities of rpLF and its positive effects on early weaned piglets have been previously evaluated, yet several features, such as the signal peptide removal, glycosylation sites and antioxidant activity of rpLF, have not been fully characterized.
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