{"title":"腺病毒(adenovirus)DNA的转录活性与宿主细胞核骨架(nuclear matrix)的关系","authors":"陈枫J. A. NickersonS. Penman 翟中和","doi":"10.1360/ZB1988-18-10-1052","DOIUrl":null,"url":null,"abstract":"本文用温和的选择性细胞抽提技术,提取腺病毒(adenovirus)感染6h的Hela细胞核内各种DNA组分,分别以腺病毒DNA的早期转录区(El a ,El b )与晚期转录区( L 2 )作探针进行Southern杂交,结果表明只有正在活跃转录着的腺病毒DNA片段才与宿主细胞核骨架紧密结合,从而说明宿主细胞核骨架可能对病毒DNA的转录有重要作用。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"252 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1988-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1360/ZB1988-18-10-1052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
本文用温和的选择性细胞抽提技术,提取腺病毒(adenovirus)感染6h的Hela细胞核内各种DNA组分,分别以腺病毒DNA的早期转录区(El a ,El b )与晚期转录区( L 2 )作探针进行Southern杂交,结果表明只有正在活跃转录着的腺病毒DNA片段才与宿主细胞核骨架紧密结合,从而说明宿主细胞核骨架可能对病毒DNA的转录有重要作用。
本文用温和的选择性细胞抽提技术,提取腺病毒(adenovirus)感染6h的Hela细胞核内各种DNA组分,分别以腺病毒DNA的早期转录区(El a ,El b )与晚期转录区( L 2 )作探针进行Southern杂交,结果表明只有正在活跃转录着的腺病毒DNA片段才与宿主细胞核骨架紧密结合,从而说明宿主细胞核骨架可能对病毒DNA的转录有重要作用。
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