[Acute-phase changes in ceruloplasmin after gamma-ray irradiation].

Abstract

The change of ceruloplasmin level of blood serum of guinea-pigs was studied on the 12th h after irradiation with gamma-rays from 60Co, using doses of 1-10 Gy, power of the dose of 0.68 Gy/min. The enzyme level was raised by the increase of the irradiation dose as it was reduced by the dose of 10 Gy in comparison with the control animals. This was probably due to inactivation of the molecules of ceruloplasmin by radicals and peroxides, obtained by this hyperlethal dose. Sera proteins showed a change in the increase of the dose: a reduction of albumins and an increase in beta- and gamma-globulins. Alpha-2-globulins, with which ceruloplasmin moved electrophoretically at low doses, showed slowly a slow increase in comparison with the control value, but at high doses-phase globulins. Oxidase activity of ceruloplasmin was determined by 01 todianisidine, but sera proteins-by agarose electrophoresis. The level of s ceruloplasmin in animals, irradiated by LD50 = 4.1 Gy, showed chronological change-an increase at early periods, maximum on the 12th h and a reduction at late periods close to the normal range. The level of serum copper followed the change in the enzyme level by analogy.