Eksperimentalna meditsina i morfologiia最新文献

The aim of the study is to examine influence of r-Hu-Ero on megakaryocytopoiesis in rats with depressed by dialysable substances thrombocytopoiesis. Experimental animals are male Wistar rats, treated with bidestilled water, used dialysis solution and r-Hu-Ero. We investigate bone marrow of experimental rats. More expressed increase of megakaryocyte count "pro mile" in rats with depressed megakaryocytopoiesis, as well as influence on megakaryocyte maturation for shorter period of time, provide evidence to be accepted that megakaryocytopoiesis, depressed by dialysable substances is more sensitive to stimulating effect of r-Hu-Ero. The reason for this enhanced sensitivity at present appear unclear.

Genotoxicity of the Bulgarian drug gastrophensin was studied by using a battery of two genotoxicity assays "in vitro" - Salmonella/mutation assay and "in vivo" - the rodent bone marrow micronucleus test. Mutagenicity of water solution of gastrophensin towards Salmonella "in vitro" - the rodent bone marrow micronucleus test. Mutagenicity of water solution of gastrophensin towards Salmonella "in vitro" was tested in five mutant, histidine auxotrophic strains - TA 1535, TA 1537, TA 1538, TA 98 and TA 100 without and in the presence of metabolic activation (+/- S9) at concentration of 0.4, 2 and 10 mg center dot ml-1. Gastrophensin did not induce mutagenic response in the Salmonella/mutation assay in a range of tested concentrations in both series of assays (+/- S9). Gastrophensin did not induce micronuclei in bone marrow cells of male C57Bl6 mice at 24, 48 and 72 hours after single oral treatment with 236 mg center dot kg-1 (80% DL50 oral, mice) and 118 mg center dot kg-1 (40% DL50 oral, mice). Based on the present data a conclusion of the lack of mutagenicity and of carcinogenic potency of gastrophensin was made.

Experiments were carried out on longitudinal and circular smooth-muscle strips isolated from the ileum and colon of healthy dogs (controls) and of dogs with local peritonitis. The changes in the contractile responses of the strips to carbachol administered cumulatively were studied, and the EC50 and pD2 values were calculated from the dose-response curves. For the colonic longitudinal strips the EC50 was 6 x 10(-8)M and the pD2 was 7.58. The pD2 for the ileal longitudinal strips was 7.83. The pD2 value for the circular strips from the ileum and colon was 7.19 and 8.87 respectively. In the dogs with local peritonitis, the sensitivity of the longitudinal strips to carbachol was higher (the pD2 for the ileum was 7.86 and for the colon-7.21) as compared to the circular strips (the pD2 for the ileum was 6.22 and for the colon-6.65). The affinity of the cholinoreceptors in the ileal circular strips to carbachol was decreased as compared to controls.

30 different lots (batches) of insulin substances ("Pharmachim" Ltd.) are analysed and the contents of somatostatin is established. The middle quantitative values and the standard deviations are specific. Though that monocomponent and bovine conventional insulin substances pertain to groups of different purity they exhibit highest purity but also greatest variability, while single-peak and porcine conventional insulins show less purity but these groups are more homogenous.

The authors examined the liver of 20 rabbits "Newzeland white" and "Chinchila", undergone atherogenic diet with cholesterol (0.3-0.5 g/kg body weight) for 30 and 60 days. They determined cholesterol, lipids, triglicerides and beta-lipoproteins in the serum. The morphologic study revealed accumulation of cholesterol in the hepatocytes, fibrosis, proliferation of the bile ducts and signs of hepatic cirrhosis.

A total of 126 white male Wistar rats under subacute conditions were studied. Of them, 76 were perorally treated with AlCl3 in a dose of 3 mg/kg b.w. daily for 40 days. Activities of acid phosphatase (AP), succinate dehydrogenase (SDH), adenosine triphosphatase (ATP-ase) and the contents of glycogen, glucoproteins and RNA were dynamically followed-up in the liver and kidney. Serum activities of ASAT and ALAT were estimated by Borhringer's tests. Morphological changes were histochemically investigated. There was an initial elevation followed by reduction mostly manifested for SDH and ATP-ase and an increase of AP at the end of trial. Histochemical data argued for disorders of protein and carbohydrate metabolism. Morphological alterations more outlined in the liver became more severe until the end of the experiment.

The rabbit pancreatic structure at histological and ultrastructural level, in different intervals after ligature of pancreatic duct, was studied. Changes in the exo- and endocrine parenchyma were observed. Canalicular structures and conjunctive tissue replace the destroyed acinar cells. In some of the islets the B cells are degranulated or necrotic. Some islets contain infiltrations of lymphocytes, macrophages and granulocytes. Cavities formed by necrotic endocrine cells could appear. The A and B cells proliferation is observed in the new-formed clusters of endocrine cells and ductless. The mechanism of this process is similar to that of the islet formation during the embryonic development or in nesidioblastosis, but they are never transformed in the definitive islet of Langerhans.

A Growth-related protein kinase's activity was studied in clinically affected and unaffected psoriatic epidermis. This activity was about 3 fold higher in clinically affected skin. Additionally, the unaffected psoriatic epidermis shows higher activity than the normal one. We conclude that the Growth-related protein kinase might be involved in the pathogenesis of psoriasis.

Pit cells are morphological equivalent of large granular lymphocytes with NK activity. The aim of our study is to define the morphological changes of pit cells in the liver of the rat after stimulation with the biological response modifier Polyerga. Polyerga was applied intramuscularly in a single dose of 6 mu g per kg body weight. The animals were perfused on the 24-th hour, 48-th hour, on the 4-th day and on the 7-th day after injection. The perfusion was done through the portal vein and the liver was processed for electron microscopical cytochemistry for endogeneous peroxidase demonstration. A 3-to-4-fold increase in the number of pit cells was observed within a period of 1 to 4 days after injection. Pit cell granules were negative for endogeneous peroxidase. After Polyerga stimulation pit cells showed a tendency of orientation of their organelles toward the contact area with Kupffer and endothelial cells. These changes are considered to represent the morphological expression of rat liver pit cell activation.

The effect of acute and chronic ASA administration on stomach and duodenal mucosa was studied on white male Wistar rats. ASA (250 mg/kg b.w.) was administered by esophageal intubation in a single dose, and the rats were sacrificed at 2, 6, 24 hours, or after two, three, ten and twenty days, 24 hours after the last ASA application. Two types of erosions were found in the first 24 hours: superficial and deep. Superficial erosions showed advanced healing by the 24th hour. Deep erosions appearing between 6 and 24 hours from the beginning of experiment became deeper, after two and three days ASA application. They had morphological features of chronic ones by the 10-20th day. ASA resorbtion was most expressed by the 3rd day from the experimental onset (plasma concentration 0.348 +/- 0.052 g/l). The resorbtion was decreased in chronic experiment, and plasma concentration (0.236 +/- 0.046 g/l, 0.244 +/- 0.045 g/l) showed statistically reliable lower values.