DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR CONDENSED TANNIN EXTRACTS OBTAINED FROM THE BARK OF FOUR TREE SPECIES USING HPLC

WOOD RESEARCH 66(2): 2021 Pub Date : 1900-01-01 DOI:10.37763/wr.1336-4561/66.2.171182

Faustino Ruiz Aquino, Rossy Feria Reyes, J. R. Quiñones, Wenceslao Santiago García, Mario Ernesto Suárez Mota, Héctor Hugo Esquivel Reyes

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引用次数: 4

Abstract

Herein, we evaluated the content of condensed tannins present in the bark of four tree species that are Quercus laurina Humb. & Bonpl., Quercus crassifolia Humb. & Bonpl., Arbutus xalapensis Kunth, and Prunus serotina Ehrn. An analytical method using high-performance liquid chromatography (HPLC) for condensed tannin extracts was developed and validated. Also, the aqueous extracts were analyzed by Fourier transform infrared spectroscopy (FTIR). Based on the Stiasny number, A. xalapensis, and Q. laurina represent an important source of condensed tannins, which may be subject to exploitation. Using infrared spectroscopy, it was observed that tannins do not show an important signal of carbonyl groups (aromatic esters) with respect to high purity catechin. Furthermore, the band of the hydroxyl group is less pronounced in tannins, because various hydroxyl are interacting with each other. However, it can be seen that the method of extraction of wood tannins developed in this work, presents satisfactory results.

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四种树种树皮浓缩单宁提取物的高效液相色谱分析方法的建立与验证

在这里，我们评估了浓缩单宁的含量存在于四种树种的树皮是栎下叶。& Bonpl。柏树(Quercus assifolia Humb)& Bonpl。杨梅、胡杨梅、樱李。建立了高效液相色谱法测定浓缩单宁提取物的方法，并进行了验证。并用傅里叶变换红外光谱(FTIR)对水提物进行了分析。从Stiasny数量上看，xalapensis和laurina是浓缩单宁的重要来源，具有开发利用的潜力。红外光谱分析表明，相对于高纯度儿茶素，单宁中不存在羰基(芳香酯)的重要信号。此外，在单宁中羟基的带不太明显，因为不同的羟基相互作用。然而，可以看出，本工作所开发的提取木材单宁的方法，取得了令人满意的结果。

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WOOD RESEARCH 66(2): 2021

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