High‐toughness Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells

Biopolymers Online Pub Date : 2003-01-27 DOI:10.1002/3527600035.BPOL8005

C. Karatzas, N. Chrétien, F. Duguay, Annie Bellemare, J. Zhou, A. Rodenhiser, Shafiul Islam, C. Turcotte, Yue-Jin Huang, A. Lazaris

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引用次数: 12

Abstract

Introduction Historical Outline Production of rc-Spider Silk Proteins Over-expression of Soluble rc-Spider silk Proteins in Mammalian Cells Secretion of rc-Dragline Spider Silk proteins Large-scale Production of rc-Spider Silk Proteins Purification and Characterization of rc-Spider Silk Proteins Production of rc-Spider Silk Proteins in the Milk of Transgenic Animals Generation of Transgenic Mice for the Spider Dragline Silk Genes Mammary Gland Production of Spider Dragline Silk Proteins Recombinant Spider Silk-based Fiber Spinning Spin Dope Preparation, Fiber Spinning and Characterization Fiber Post-Spinning Treatment Perspective on the Formation of Silk Fibers and the Spinning Process Applications of rc-Spider Silk Fibers, and Prospects Patents Chronological Summary of Patents Conclusions Acknowledgments Keywords: spider silk; genetic engineering; recombinant silk protein; fiber spinning; expression; mammalian cells; transgenic mice; spin dope; fibers

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Biopolymers Online

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