{"title":"Simultaneous autoradiographic and immunohistochemical localization of estrogen and gonadotropin in the rat pituitary.","authors":"D. Keefer, W. Stumpf, P. Petrusz, M. Sar","doi":"10.1097/00006254-197508000-00016","DOIUrl":null,"url":null,"abstract":"A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized \"gonadotropes\". Most, but not all, \"gonadotropes\" show significant nuclear concentration of radioactivity. A large percentage of non-\"gonadotropes\" also show nuclear uptake of label.","PeriodicalId":101557,"journal":{"name":"The American journal of anatomy","volume":"79 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The American journal of anatomy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1097/00006254-197508000-00016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
Abstract
A combined autoradiographic and immunohistochemical technique is introduced which is suitable to simultaneously localize hypophyseal sites of nuclear (3-H)-estradiol uptake and anti-hCG-characterized "gonadotropes". Most, but not all, "gonadotropes" show significant nuclear concentration of radioactivity. A large percentage of non-"gonadotropes" also show nuclear uptake of label.
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