Transmission of Rickettsia tsutsugamushi from Apodemus speciosus, a wild rodent, to larval trombiculid mites during the feeding process.

Abstract

Transmission of Rickettsia tsutsugamushi (Rt) from a rodent to trombiculid mites was studied. Wild rodents, Apodemus speciosus, were subcutaneously inoculated with Karp strain of Rt, and larval trombiculid mites were released on the ear lobes for feeding, 10 and 20 days after infection. Isolation of Rt was performed from individual mites or pools of 2 to 7 mites by the mouse passage method. From Apodemus 10 days after Rt infection, possibly at the time of high rickettsiaemia, the infection rates among mites were 4/44 (9.1%) or higher in Leptotrombidium fuji, 1/20 (5%) in L. pallidum and 0/41 in L. deliense. From Apodemus 20 days after infection, no mite was infected in 50 L. fuji or 7 L. pallidum possibly due to reduced rickettsiaemia. Transmission of Rt from rodent to mite was proven to occur at low probability on appropriate conditions but not by the incidental chance.