DETERMINING THE ALGAE CELL DENSITY AND TOTAL CAROTENOID CONTENT OF HETEROTROPHIC MARINE MICROALGAE - SCHIZOCHYTRIUM AND THRAUSTOCHYTRIUM ON THE COAST OF TRA VINH PROVINCE, VIETNAM

Nguyen Thi Binh Pham
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Abstract

This study aimed to determine the biomass culture capacity and total carotenoid content of heterotrophic marine microalgae genera, including the genus Schizochytrium and Thraustochytrium on the coast of Tra VinhProvince. Five strains of these genera, DH41.79, CN27, DH10, CN15, and CN47, were cultured for biomass and carotenoid content. The study showed that the cell density of CN47 was highest with different periods under three different dilution levels (10%, 20%, and 30%), with a positive relationship between cell density and dryweight of algae strains (R2 ≈ 1 > 0). Besides, the results indicated that all densities of strain CN47 showed carotenoid content of this strain (7,613 µg/kg) was significantly higher than that of the 04 remainings and previous studies. The research results contribute to the foundation for scientific works on the heterotrophic marine microalgae Thraustochytrids, which have a high potential for applications in biomass production,extraction of high-value nutritional compounds for aquaculture, towards sustainable development through replacing fish oil sourced from natural exploitation in Tra Vinh Province.
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测定越南越共省沿海异养型海洋微藻——裂囊藻和thraustochytrium的藻类细胞密度和总类胡萝卜素含量
本研究旨在测定特拉温省沿海异养型海洋微藻属(Schizochytrium属和Thraustochytrium属)的生物量培养能力和总类胡萝卜素含量。对该属5株菌株DH41.79、CN27、DH10、CN15和CN47进行生物量和类胡萝卜素含量的测定。研究结果表明,在10%、20%、30% 3种不同稀释水平下,CN47的细胞密度在不同时期最高,且细胞密度与菌株干重呈正相关(R2≈1 > 0),且各稀释浓度下,该菌株的类胡萝卜素含量(7613µg/kg)显著高于04个剩余菌株和前人研究。该研究结果为开展异养型微藻的科学研究奠定了基础,这些微藻在生物质能生产、水产养殖高价值营养化合物的提取以及通过替代来自特拉永省自然开采的鱼油实现可持续发展方面具有很高的应用潜力。
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