Biofilm forming Enterococci and their Status as Emerging Multidrug Resistant Bacteria

Z. Haruna, Aishatu Habib Idris, Maryam Idris Muhammad, N. Abdulwahab, S. Abdullahi, N. Wada
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引用次数: 2

Abstract

Urine specimens were collected aseptically and inoculated directly onto CLED and MacConkey agar medium and incubated overnight. Single target colonies were sub cultured, and identification was based on culture, morphology, and biochemical characteristics. Enterococci isolates were subjected to biofilm assay (Tube method), and resistance pattern was determined for both biofilm-formers and non-biofilm-formers. Genomic DNA was extracted using the Bioneer kit, and the gene for virulence was detected by PCR and agarose gel electrophoresis. 148 Patients having one or more urinary symptoms were the study population. The aim of the study was to determine the multidrug resistant ability of biofilm-forming Enterococci. A number of growths were encountered; Enterococci accounted for only 9.46% (14), the highest prevalence was seen in the age group 21-30 (35.71%), and females (64.29%) were more prone to enterococcal infection than their males (35.71) counterpart. There is a relationship between biofilm production and antibiotic resistance because multidrug resistant Enterococci isolate produced bands against esp gene with an average of 510kbp. Biofilm forming strains showed the highest resistance to gentamicin and penicillin (83.3%), and vancomycin can be considered a good alternative therapy in enterococcal UTIs because of its lower resistance (75%).
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形成生物膜的肠球菌及其作为新出现的多重耐药细菌的地位
无菌收集尿液标本,直接接种于ccled和MacConkey琼脂培养基上,孵育过夜。单目标菌落继代培养,并根据培养、形态和生化特征进行鉴定。对分离的肠球菌进行生物膜测定(试管法),并对形成生物膜的肠球菌和非形成生物膜的肠球菌进行耐药性分析。采用Bioneer试剂盒提取基因组DNA,采用PCR和琼脂糖凝胶电泳检测毒力基因。148例有一种或多种泌尿系统症状的患者为研究人群。该研究的目的是确定生物膜形成肠球菌的多重耐药能力。遇到了一些增长;肠球菌感染率仅为9.46%(14%),其中21 ~ 30岁患病率最高(35.71%),女性(64.29%)高于男性(35.71%)。由于多药耐药肠球菌分离物产生的esp基因条带平均为510kbp,因此生物膜的生成与抗生素耐药性之间存在一定的关系。生物膜形成菌株对庆大霉素和青霉素的耐药性最高(83.3%),万古霉素的耐药性较低(75%),可被认为是肠球菌性uti的良好替代疗法。
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