Recombinant nanobodies detecting the human cardiac troponin proteins by ELISA

Abstract

We tested the capacity of antibodies RB733, RB734, RB766, RB767, RB768, RB769, RB770, RB771, RB772 and RB773 to recognize by ELISA three different molecular forms of the human cardiac troponin T isoform 6 (cTnT): an N-terminal peptide (Nter-cTnT), the full-length cTnT protein (FL-cTnT) and the cardiac troponin I-T-C complex (ITC-complex). Our results show that RB734 specifically recognizes the N-terminal region of cTnT as well as the full-length protein alone and the ITC-complex. RB767, RB770, RB771, RB772 and RB773 recognize the full-length cTnT protein alone or in the ITC-complex but not the N-terminal peptide. RB766, RB768 and RB769 recognize the ITC-complex but not cTnT or cardiac troponin I (cTnI) proteins.