Synthesis, characterization and functional evaluation of gold nanoparticles prepared using Dovyalis abyssinica leaf extracts as reducing and surface capping agent

Abera Beyene Gebresilassie, Adam Mekonnen Engida
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Abstract

Green synthesis of nanoparticles using plants and microorganisms is biologically safe, cost effective, and environmentally friendly technology. Gold nanoparticles (Au NPs) were synthesized using aqueous extracts of leaves of Dovyalis abyssinica as reducing and surface capping agent and the catalytic activity, antibacterial action and antioxidant potential of the synthesized AU NPs were evaluated. Firstly, HAuCl4 was synthesized in the laboratory from metallic gold and hydrochloric acid using a predesigned method. Secondly, Au NPs was synthesized by mixing HAuCl4 and the plant extract at 45 oC with a digestion time of 1 h. The size of the nanoparticles was modulated by varying the ratio of the plant extract and HAuCl4 with known concentrations. The synthesized Au NPs showed strong absorption around 540 nm which lies in the characteristic absorption region of Au metal nanoparticles (520–580 nm). The X-ray diffraction spectrum of the synthesized Au NPs showed characteristic crystalline structures of gold. The scanning electron spectroscopy images of the synthesized Au NPS revealed the presence of mixed shapes predominantly of irregular shapes and a particle size analyzer displayed an average size of 63.13 nm. The fourier-transform infrared spectrum of Au NPS confirmed the presence of amine, carbonyl and hydroxyl functional groups as surface capping molecules. Although the synthesized Au NPs showed poor bacterial growth inhibition activity on two selected bacteria, it demonstrated excellent free radical scavenging activity against 2, 2-Diphenyl-2-picrylhydrazyl (DPPH) radical and good catalytic activity for degrading bromothymol blue and methyl red compounds. In contrast to the hexane and ethyl acetate extracts, the aqueous fraction was identified as powerful reducing fraction for the synthesis of Au NPs in this experiment. 
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以深草叶提取物为还原剂和表面封盖剂制备金纳米颗粒的合成、表征及功能评价
利用植物和微生物绿色合成纳米粒子是一种生物安全、成本效益高、环境友好的技术。以深海紫鸽叶水提物为还原剂和表面封盖剂合成了金纳米粒子(Au NPs),并对其催化活性、抗菌作用和抗氧化潜力进行了评价。首先,以金属金和盐酸为原料,采用预先设计的方法在实验室合成了HAuCl4。其次,将HAuCl4与植物提取物在45℃下混合,消化时间为1 h,合成金纳米粒子。在已知浓度下,通过改变植物提取物与HAuCl4的比例来调节纳米粒子的大小。所合成的金纳米粒子在540 nm附近有较强的吸收,处于金纳米粒子的特征吸收区(520 ~ 580 nm)。合成的金纳米粒子的x射线衍射谱显示出金的典型晶体结构。扫描电子能谱图显示合成的Au NPS存在以不规则形状为主的混合形状,粒度分析仪显示其平均粒径为63.13 nm。Au NPS的傅里叶变换红外光谱证实了其表面有胺基、羰基和羟基等官能团作为盖层分子。虽然所合成的Au NPs对两种选定的细菌的生长抑制活性较差,但对2,2 -二苯基-2-苦味酰肼(DPPH)自由基具有良好的清除活性,对溴百里酚蓝和甲基红化合物具有良好的催化降解活性。与正己烷和乙酸乙酯提取物相比,在本实验中,水馏分被认为是合成金纳米粒子的有力还原馏分。
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