Antibacterial Polymeric Wound Dressing Based On PVA/Graphene Oxide-Nigella Sativa-Arginine

Abstract

The number of patients with various types of skin wounds has increased due to the emergence of new skin diseases. So that the traditional dressings have fallen out of the reach of researchers and replaced with new ones. Nowadays, biopolymeric scaffolds, especially nanofibers, are widely considered for engineering applications of skin tissue and wound coating. At first, Graphene oxide was synthesized from graphite and then added to the extract of Nigella sativa and Arginine. FTIR, Zeta potential, SEM and Mapping Elemental analyzes were used to confirm the accuracy of the materials. The antibacterial properties of the materials were also evaluated by MIC, OD and disk diffusion assay. The results showed that these materials are capable of inhibiting the growth of Gram-positive and Gram-negative bacteria. Next, the PVA solution was prepared and combined with GO/NS/Arg to produce by the electro spinning process of nanofibers. FTIR and elemental mapping confirmed the presence of nanomaterials in the nanofibers. Also, tensile strength test was performed to investigate the properties of nanofibers. According to the results, the tensile strength in the presence of GO/NS/Arg showed good results and its maximum strain was 1.42. Fibroblast cells were used to evaluate cell toxicity and viability by MTT and Scratching methods. The results indicate the non-toxicity of the nanocomposite at concentration 2 (µg)/mL. Nanofibers were used to investigate wound healing in animal models, and the results indicated that these nanofibers can accelerate the wound healing process, so that after 14 days the wound healing rate was 93.126%.