[The cytochemical detection of monocytes, lymphocytes, neutrophilic and eosinophilic granulocytes by using marker enzymes].

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Abstract

Some cytochemical methods for determination of monocytes, neutrophilic and eosinophilic granulocytes were modified on the basis of manifestation of the respective marker enzymes (alpha-naphthyl-acetatesterase, naphthol-AS-D-chloracetatesterase and cyano-resistant peroxidase). The modification presented formation of economic methods by using some common reagents for identification of single esterases and peroxidases.

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[利用标记酶检测单核细胞、淋巴细胞、嗜中性粒细胞和嗜酸性粒细胞]。
根据标记酶(α -萘酰-乙酰酯酶、萘酚- as - d -氯乙酰酯酶和耐氰过氧化物酶)的表现,对测定单核细胞、中性粒细胞和嗜酸性粒细胞的细胞化学方法进行了改进。本文提出了用几种常用试剂对单一酯酶和过氧化物酶进行经济鉴定的方法。
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