Evaluation of Hepatoprotective Activity of Ethanolic Extract of Root of Prunus Persica on Paracetamol Induced Hepatotoxic in Rats

Abstract

Objectives: The Objective of the present study is evaluation of hepatoprotective activity of root extract of Prunus persica on paracetamol induced hepatotoxic in rats. Methods: The roots of Prunus persica was dried under shade and then powdered and extracted with ethanol and the resultant extract was subjected for phytochemical analysis to identify different phytoconstituents. The effect of ethanolic extract of roots of Prunus persica on hepatotoxicity was evaluated by using experimental model of paracetamol induced liver necrosis. In this model the common parameter determined were physical parameter like wet liver weight and biochemical parameter like SGOT, SGPT, ALP and Total Bilirubin changes in liver. Histopathological changes in liver were assessed. Results: Phytochemical investigation of ethanolic extract of roots of P. persica revealed the presence of Carbohydrates, Tannins, Amino acids, Flavonoids, Steroids and Cardiac glycosides. Oral administration of Prunus persica extract (400 mg/kg) showed dose related hepatoprotective activity in Paracetamol induced liver necrosis. The parameters SGOT, SGPT, ALP and Total Bilirubin level were increased in paracetamol treated group whereas the treatment group like standard and extract decreased. The histopathological examination of the liver in the hepatotoxic animals in Paracetamol model shows liver parenchyma with distorted architecture. Some of the hepatocytes show degenerative changes, while some show apoptosis. Which were less in the treated group and regenerative hepatocytes show in treatment group? Conclusion: A significant hepatoprotective activity of ethanolic extract of Prunus persica observed in the present investigation may be due to synergistic potentiate action of its phytoconstituents since they contain a diverse array of active principles which are able to target multiple mechanisms involved in the pathophysiology of hepatotoxicity