{"title":"Localization-based spot decoding for accurate multiplexed RNA-FISH at scale","authors":"Alexis Coullomb, Peter T. Brown, D. Shepherd","doi":"10.1364/boda.2023.dth2c.4","DOIUrl":null,"url":null,"abstract":"A new spot decoding method allows to infer at scale and more accurately mRNA species from 3D spots localized with a physics-based approach in big datasets of barcoded RNA-FISH experiments.","PeriodicalId":111173,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1364/boda.2023.dth2c.4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A new spot decoding method allows to infer at scale and more accurately mRNA species from 3D spots localized with a physics-based approach in big datasets of barcoded RNA-FISH experiments.
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