Viability of Spermatozoa in Goat's Liquid Semen after Adding Sweet Orange Essential Oil to The Andromed Extender

S. Sitepu, J. Marisa, Aditiya Amanda Rianto
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Abstract

Aims: To find out the percentage of live spermatozoa after diluting the goat's liquid semen using an andromed extender plus sweet orange essential oil. Study Design: Randomized Block Design. Place and Duration of Study: Sample: Galang, Deli Serdang Regency, Indonesia, between December 2022 and January 2023. Methodology: The research procedure starts with the preparation of semen extender, collection of fresh semen, dilution of semen and equilibration. This  research obtained  conducted  using  a  Randomized  Block  Design  consisting  of  5  treatment  levels  and  five replications. Semen storage using 3 Bucks, which has done for three days. As a treatment is the addition of sweet orange essential oil as much as (P0) 0%, (P1) 0.25%, (P2) 0.5%, (P3) 0.75% and (P4) 1% on the andromed extender. The observed variable was percentage viability spermatozoa evaluated before equilibration and after equilibration. Results: The results showed that the addition of sweet orange essential oil had a not significant effect (P <0.01) to viability of spermatozoa. The results of adding sweet orange essential oil to the extender (after equilibration) were 80% (P0), 80% (P1), 80% (P2), 80% (P3) and 81% (P4). Conclusion: Adding sweet orange essential oil to andromed had no effect on the viability of goat spermatozoa in liquid semen.
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添加甜橙精油对山羊液体精液中精子活力的影响
目的:用雄性化扩展剂加甜橙精油稀释山羊的液体精液,测定活精子的百分率。研究设计:随机区组设计。研究地点和时间:样本:加朗,Deli Serdang摄政,印度尼西亚,2022年12月至2023年1月。方法:研究程序从精液填充剂的制备、新鲜精液的收集、精液的稀释和平衡开始。本研究采用随机区组设计,包括5个处理水平和5个重复。用三块钱储存精液,已经用了三天了。作为处理,甜橙精油分别为(P0) 0%、(P1) 0.25%、(P2) 0.5%、(P3) 0.75%和(P4) 1%。观察到的变量是平衡前和平衡后精子存活率的评估。结果:结果表明,添加甜橙精油对精子活力无显著影响(P <0.01)。在膨胀剂中加入甜橙精油(经平衡后)的结果分别为80% (P0)、80% (P1)、80% (P2)、80% (P3)和81% (P4)。结论:加入甜橙精油对羊精液中精子活力无影响。
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