{"title":"Extracellular Polyhydroxyalkanoate (PHA) Depolymerases: The Key Enzymes of PHA Degradation","authors":"D. Jendrossek","doi":"10.1002/3527600035.BPOL3B03","DOIUrl":null,"url":null,"abstract":"Introduction \nHistorical Outline \nIdentification and Isolation of Extracellular d-Poly(HA)-Degrading Microorganisms \nCharacterization of Poly(HA)-Degrading Microorganisms \nBiochemical Properties of Extracellular d-Poly(HA) Depolymerases \nMolecular Biology and Functional Analysis of d-Poly(3HASCL) Depolymerases \nPhaZ7, a new Type of Thermoalkalophilic Hydrolase of P. lemoignei with high Specificity for Amorphous Poly(HASCL) \nMolecular Biology and Functional Analysis of d-Poly(HAMCL) Depolymerases \nEnantioselectivity and Hydrolysis Products of Poly(HA) Depolymerases \nRegulation of Poly(HA) Depolymerase Synthesis \nInfluence of Physico-chemical Properties of the Polymer on its Biodegradability \nOutlook and Perspectives \nPatents \nAcknowledgments \n \n \nKeywords: \n \nPHA depolymerase; \nPHB depolymerase; \nPHO depolymerase; \nPaucimonas lemoignei; \nPseudomonas lemoignei; \nPseudomonas fluorescens GK13; \npoly(HA); \nnative PHA; \ndenatured PHB; \nartificial PHA; \natactic PHA; \nlipase; \nserine hydrolase; \ncatalytic triad; \nsubstrate-binding domain; \ncarboxyesterase","PeriodicalId":165163,"journal":{"name":"Biopolymers Online","volume":"69 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2002-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"49","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biopolymers Online","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/3527600035.BPOL3B03","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 49
Abstract
Introduction
Historical Outline
Identification and Isolation of Extracellular d-Poly(HA)-Degrading Microorganisms
Characterization of Poly(HA)-Degrading Microorganisms
Biochemical Properties of Extracellular d-Poly(HA) Depolymerases
Molecular Biology and Functional Analysis of d-Poly(3HASCL) Depolymerases
PhaZ7, a new Type of Thermoalkalophilic Hydrolase of P. lemoignei with high Specificity for Amorphous Poly(HASCL)
Molecular Biology and Functional Analysis of d-Poly(HAMCL) Depolymerases
Enantioselectivity and Hydrolysis Products of Poly(HA) Depolymerases
Regulation of Poly(HA) Depolymerase Synthesis
Influence of Physico-chemical Properties of the Polymer on its Biodegradability
Outlook and Perspectives
Patents
Acknowledgments
Keywords:
PHA depolymerase;
PHB depolymerase;
PHO depolymerase;
Paucimonas lemoignei;
Pseudomonas lemoignei;
Pseudomonas fluorescens GK13;
poly(HA);
native PHA;
denatured PHB;
artificial PHA;
atactic PHA;
lipase;
serine hydrolase;
catalytic triad;
substrate-binding domain;
carboxyesterase
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