The application of Iron Oxide magnetic nanoparticles to improve the binding efficiency of the IgG and Thiol SAMs

Abstract

Superparamagnetic nanoparticles have been fabricated successfully, which are composed of Fe3O4 by using wet chemical co-precipitate method. The nanoparticles were functionalized on the surface by conjugated with the abti-Rabbit IgG. In the recently research, we try to use this system to approach the work which is to increase the reaction efficiency between antibodies and antigens by applying magnetic fields to alternate the binding samples in solution. The applied magnetic fields are produced by electro-magnets with AC or DC input. In the preliminary experiment, the antibodies were reacted with the carboxyl group Thiol SAMs that were modified on the glass chip with gold film. The surface plasma resonance (SPR) was used to detect the binding number of antibodies on the chip. The binding efficiency could be further calculated. The results showed the binding efficiency could be improved by using superparamagnetic nanoparticles with applying magnetic fields.