The state of intermediate metabolism and antioxidant protection of the organism of quail during denitrifation

Ch. Gaytov, V. Gappoeva, I. Ktsoeva, R. Temiraev, S. Kozyrev
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Abstract

Purpose: the study of the exposure of the prototype of sagging and the phospholipid complex of lecithin for the intermediate exchange and enzymatic activity of the anti -radical protection of the body of quails in the presence of a subtoxic dose of nitrates in the compound feeds.Materials and methods. To carry out a scientific and production experiment, meat caves "Pharaoh" were used. From per diem, 4 groups of experimental birds of 50 goals in each were formed on the principle of groups of groups analogues. Compound feeds used for feeding are quittered, grown for meat, were prosperous in the presence of nitrates. With this in mind, for the purity of the experiment, they additionally introduced sodium nitrate in a dose of 40 g/t feed in their composition for all groups in the role of the source of these xenobiotics. At the same time, the presence of nitrates in the diet in a subtoxic dose was ensured. A sagitol’s sagitot at a dose of 1250 g/t, for the 3rd group-1000 g/t lacitin, for the 4th-1250 g/t of the sagitol test + 1000 g/t Lecin, was added to the feed. The 1st group was controlled - for birds of these groups in compensation, sagitol and lecithin were not added to determine the effectiveness of denitrification studied the basic morphological and biochemical parameters of blood and the activity of enzymes of anti -radical protection of the body of quails (5 heads in the group) aged 42 days.Results. In the course of the studies, it was first established that joint supplements in the diets of produces of produces of sagol at a dose of 1.25 kg/t of feed and lecithin at a dose of 1.0 kg/t food contributed to effective denitrification in the body of the plyona breed. This was manifested in a reliable (p <0.05) increase in the bird of the 3rd experimental group regarding control in the liquid internal environment of the number of red blood cells and hemoglobin, as well as in a decrease in the concentration of metghemoglobin by 45.17% (p <0.05) . With joint additives in the diets of probiotic and lecithin, an improvement in the state of natural resistance and antioxidant protection of the poultry body was observed, which was manifested in an increase in the quails of the 3rd experimental group regarding control of lysozymic activity by 2.99% (p <0.05), bacticipid by 11.09% (p <0.05) and the activity of glutathioneperoxidase - by 43.22% (p <0.05) with a decrease in catalase activity - by 17.46% (p <0.05). Under the influence of these drugs, the quails of 3 of the experimental group observed the best level of denitrification, which in the blood serum increased the amount of uric acid by 42.56% (p <0.05) due to a decrease in nitrates by 50.54% (p <0 , 05) and nitrites - by 60.55% (p <0.05).
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鹌鹑反硝化过程中机体的中间代谢状态及抗氧化保护
目的:研究复合饲料中亚毒性剂量硝酸盐对鹌鹑松弛原型和卵磷脂复合物的暴露对其机体抗自由基保护的中间交换和酶活性的影响。材料和方法。为了进行科学和生产实验,使用了肉洞“法老”。从每天开始,按群对群类比的原则组成4组实验鸟,每组50只。用于饲养的复合饲料是废弃的,用于肉类的,在硝酸盐的存在下是繁荣的。考虑到这一点,为了实验的纯度,他们在所有组的组成中额外添加了40 g/t剂量的硝酸钠,作为这些异种抗生素的来源。同时,饮食中硝酸盐的亚毒性剂量得到了保证。在饲料中添加剂量为1250 g/t的矢状体,第三组为1000 g/t乳糜素,第四组为1250 g/t矢状体+ 1000 g/t卵磷脂。第一组为对照,在补偿组中不添加矢状醇和卵磷脂以测定反硝化效果,研究了42日龄鹌鹑(组中5头)血液的基本形态生化参数和机体抗自由基保护酶活性。在研究过程中,首先确定了在饲料中联合添加1.25 kg/t饲料剂量的鼠尾草和1.0 kg/t饲料剂量的卵磷脂有助于蒲利纳品种体内有效的反硝化作用。这表现在第三实验组的鸟在液体内环境中红细胞和血红蛋白的数量与对照组相比有可靠的增加(p <0.05),高血红蛋白的浓度下降了45.17% (p <0.05)。在饲粮中添加益生菌和卵磷脂,可改善家禽机体的自然抗性和抗氧化保护状态,其中第3试验组鹌鹑的溶菌酶活性提高了2.99% (p <0.05),乳酸酶活性提高了11.09% (p <0.05),谷胱甘肽过氧化物酶活性提高了43.22% (p <0.05),过氧化氢酶活性降低了17.46% (p <0.05)。在这些药物的作用下,试验组3只鹌鹑的反硝化水平最高,血清中硝酸盐和亚硝酸盐分别降低50.54% (p <0.05)和60.55% (p <0.05),尿酸升高42.56% (p <0.05)。
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