{"title":"Migratory response of human orbital fat-derived mesenchymal stem cell encapsulated in collagen gel","authors":"H. Diao, C. Yu, B. Chan","doi":"10.1109/NANOMED.2010.5749841","DOIUrl":null,"url":null,"abstract":"The aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2–3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2–6 OFSCs into collagen type I microspheres, followed by a well-established cell migration assay under attraction of two kinds of chemokines −10 ng/ml Fractalkine or 50 ng/ml SDF-1β. After staining and counting, we found that after 3D collagen barrier selection, migrated OFSCs have more sensitive response to the induction of these two chemokines.","PeriodicalId":446237,"journal":{"name":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","volume":"15 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2010 IEEE International Conference on Nano/Molecular Medicine and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/NANOMED.2010.5749841","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2–3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2–6 OFSCs into collagen type I microspheres, followed by a well-established cell migration assay under attraction of two kinds of chemokines −10 ng/ml Fractalkine or 50 ng/ml SDF-1β. After staining and counting, we found that after 3D collagen barrier selection, migrated OFSCs have more sensitive response to the induction of these two chemokines.
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