Estimation of the Digestibility of Isomaltooligosaccharide and Its Hydrogenated Derivative Using the Modified Prosky Method(Enzymatic - LC method)

Abstract

The digestibility of oligosaccharides was studied by the modified Prosky method (Enzymatic-LC method), which was officially adopted the determination of low-molecular soluble dietary fiber. When the low-digestible saccharide, isomaltooligosaccharide (IMO) and its hydrogenated derivative (IMO-H) were applied to the methods, the hydrolysis ratios were relatively lower than those of starch hydrolysate, although the ratios de pended on the concentrations of enzyme and substrate. Changes in saccharides composition of IMO or IMO H were almost the same as those which were observed in our previous studies using intestinal mucosa en zyme. These results suggested that the Enzymatic-LC method could be useful for estimation of digestibility for some kinds of oligosaccharide, derived from starch. Further improvements of the enzymatic digestion sys tem, which could simulate a human gastric enzyme mixture, will be able to establish an available method for various types of oligosaccharides.