DNA barcodes for cultivar identification of Thailand Tamarindus indica.L

Benchaporn Srisuvoramas, Surachest Aiumsamang, Thep Piamalung, S. Sansenya, Lertharong Sripanom
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引用次数: 1

Abstract

This study aims to investigated Tamarindus indica.L. chloroplast rbcL gene for ribulose-1,5-bisphosphate carboxylase/oxygenase, large subunit as the molecular marker to identified the cultivar level of Tamarindus indica.L. in Thailand. The partial rbcL gene of 19cultivars were sequences approximately 611-712 bp. The sequence alignment and Phylogenetic Tree analysis were generated by Clustal W and NJ methods. The result of nucleotide sequence alignment of partial rbcL gene for all cultivars were divided into 19 groups. The different of these groups similar to the different of local name of Tamarind cultivars in Phetchabun Province, Thailand. Base on these results indicated that 19 cultivar DNA Barcodes can supported the local opinions act as the scientific data to identification of tamarind cultivars in Thailand.
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泰国柽柳品种鉴定的DNA条形码。l
以柽柳为研究对象。利用叶绿体rbcL大亚基核酮糖-1,5-二磷酸羧化酶/加氧酶基因作为分子标记,鉴定柽柳品种水平。在泰国。19个品种的rbcL部分基因序列约为611 ~ 712 bp。采用cluster W和NJ方法进行序列比对和系统进化树分析。所有品种部分rbcL基因的核苷酸序列比对结果分为19组。这些类群的差异与泰国碧差汶省罗望子品种的地方名称差异相似。结果表明,19个品种的DNA条形码可以支持当地意见,作为泰国罗望子品种鉴定的科学数据。
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