3P007 Systematic structural study of single amino acid insertion mutants of YFP(01A. Protein: Structure,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Abstract

YFP is a mutant derivative of green fluorescent protein, and is widely used as a fluorescent indicator in molecular biology. Recently we found that YFP mutant variants with single amino acid insertion in β7 showed various fluorescence responses to environmental change. The fluorescence responses depend on the nature of the inserted amino acid residue. To elucidate the molecular mechanism how the insertion affects the fluorescent property, we prepared twenty single amino acid insertion variants of YFP and solved the structures. Here we show the structures of eighteen mutant YFPs. They show unexpected conformational variation in β7 dependent on the inserted residue. We will discuss the relationship between the mutant structure and the fluorescent property.