SPECTRAL-SELECTIVE LASER-INDUCED AUTOFLUORESCENT MICROSCOPY OF POLYCRYSTALLINE FRACTION OF THE HUMAN VITREOUS BODY IN DIAGNOSTICS TIME SINCE DEATH

Abstract

laser-induced microscopy the of the human body was performed according to standard methods, subsequently performed a statistical analysis of the results. Results. Analysis of the obtained data, which illustrate the necrotic changes of the polycrystalline component of the vitreous body layers, revealed a linear range of changes in the set of all statistical moments of 1 - 4th orders, which characterize the coordinate distributions of the autofluorescence intensity of the polycrystalline structure of the vitreous body for 36 hours after death. The most sensitive to necrotic changes in the polycrystalline structure were statistical moments of the 3rd - 4th orders, which increase with increasing postmortem interval from 0.22 to 1.27 and from 0.11 to 1.91, respectively. Conclusions. The method of spectral-selective laser-induced autofluorescence microscopy of the polycrystalline fraction of the vitreous body layers provides a sensitivity range of 36 hours. with the accuracy of determining the time since death in 15 minutes.