{"title":"Separation imaging of DNA fragments in poly (dimethylsiloxane) microchip","authors":"Changchun Liu, D. Cui","doi":"10.1109/ICIA.2004.1373352","DOIUrl":null,"url":null,"abstract":"Poly (dimethylsiloxane) (PDMS) had been extensively used as excellent microchip material due to its particular advantages. In this paper, Laser-induced fluorescence detection system for visualizing microchip electrophoresis had been constructed and a series of electrophoresis operation of DNA fragments labeled by SYBR Green I were imaged by charge coupled device (CCD) camera. We investigated the influence of the sieving matrix concentration and the electric field strength on the formation of sample injection plug. PCR markers were well separated in PDMS microchip.","PeriodicalId":297178,"journal":{"name":"International Conference on Information Acquisition, 2004. Proceedings.","volume":"78 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2004-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Conference on Information Acquisition, 2004. Proceedings.","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICIA.2004.1373352","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Poly (dimethylsiloxane) (PDMS) had been extensively used as excellent microchip material due to its particular advantages. In this paper, Laser-induced fluorescence detection system for visualizing microchip electrophoresis had been constructed and a series of electrophoresis operation of DNA fragments labeled by SYBR Green I were imaged by charge coupled device (CCD) camera. We investigated the influence of the sieving matrix concentration and the electric field strength on the formation of sample injection plug. PCR markers were well separated in PDMS microchip.
聚二甲基硅氧烷(PDMS)由于其独特的优点而被广泛用作优良的微芯片材料。本文构建了用于微芯片电泳可视化的激光诱导荧光检测系统，并利用电荷耦合器件(CCD)相机对SYBR Green I标记的DNA片段进行了一系列电泳操作。研究了筛分基质浓度和电场强度对进样塞形成的影响。PCR标记在PDMS芯片上分离良好。
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