Neem oil against Aeromonas hydrophila infection by disrupting quorum sensing and biofilm formation.

IF 2 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biofouling Pub Date : 2023-08-01 Epub Date: 2023-11-20 DOI:10.1080/08927014.2023.2279998

Shengping Li, Qiuhong Yang, Bo Cheng, Yongtao Liu, Shun Zhou, Xiaohui Ai, Jing Dong

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Abstract

Aeromonas hydrophila is an opportunistic pathogen that can cause a number of infectious diseases in fish and is widely distributed in aquatic environments. Antibiotics are the main approach against A. hydrophila infections, while the emergence of resistant bacteria limits the application of antibiotics. Here, quorum-sensing (QS) was defined as the target and the inhibitory effects of neem oil against QS of A. hydrophila was studied. The results showed that neem oil could dose-dependently reduce aerolysin, protease, lipase, acyl-homoserine lactones (AHLs), biofilm and swarming motility at sub-inhibitory concentrations. Results of real-time PCR demonstrated that neem oil could down-regulate the transcription of aerA, ahyI and ahyR. Moreover, neem oil showed significant protections to A549 cells and a fish infection model. Taken together, these results indicated that neem oil could be chosen as a promising candidate for the treatment of A. hydrophila infections.

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印度楝油通过破坏群体感应和生物膜形成来对抗嗜水气单胞菌感染。

嗜水气单胞菌是一种条件致病菌，可引起鱼类多种传染病，广泛分布于水生环境中。抗生素是治疗嗜水单胞菌感染的主要方法，但耐药菌的出现限制了抗生素的应用。本文以群体感应(QS)为靶点，研究了印楝油对嗜水单胞菌QS的抑制作用。结果表明，在亚抑制浓度下，印楝油可以剂量依赖性地降低气溶酶、蛋白酶、脂肪酶、酰基同丝氨酸内酯(AHLs)、生物膜和蜂群运动。实时荧光定量PCR结果显示，印楝油可下调aerA、ahyI和ahyR的转录。此外，印楝油对A549细胞和鱼感染模型有显著的保护作用。综上所述，这些结果表明，印楝油可以作为治疗嗜水单胞菌感染的有希望的候选者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.