Human macrophage immunometabolism regulator (MACIR) in patients with periodontitis

IF 2.5 4区医学 Q3 IMMUNOLOGY Immunobiology Pub Date : 2023-11-01 DOI:10.1016/j.imbio.2023.152760

Karol Serwin , Małgorzata Kozak , Małgorzata Mazurek-Mochol , Katarzyna Piotrowska , Michał Czerewaty , Andrzej Pawlik

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Abstract

Objective

Periodontitis is a local inflammatory reaction caused by bacterial infection in which immune cells, including macrophages, are involved. Recent studies have shown that an important regulator of macrophage function is the human macrophage immunometabolism regulator (MACIR). This gene has been shown to play a key role in modulating the immune response by affecting the activity of fibroblasts and macrophages.

In this study, we investigated the expression of MACIR in the gingival tissues of patients with periodontal disease, as well as the effect of IL-1β and TNF-α on the expression of MACIR gene and protein in human gingival fibroblasts.

Methods

MACIR mRNA expression in gingival tissue samples was determined using Real-time PCR. Expression of MACIR protein was determined using immunofluorescent staining and western blotting.

Results

The MACIR mRNA expression in gingival tissue samples in patients with periodontitis was statistically significantly lower than in gingival tissue samples from healthy controls (p = 0.009). The stimulation of human gingival fibroblasts with IL-1β and TNF-α resulted in a statistically significant decrease of MACIR gene mRNA expression. In western blotting and immunofluorescent analysis, we confirmed that the stimulation of the primary culture of human gingival fibroblasts by both IL-1β and TNF-α decreases the expression of MACIR protein.

Conclusion

The results of the study suggest that MACIR is an important regulator of the inflammatory process in patients with periodontitis. Decreased expression of the MACIR gene may activate macrophages to secrete mediators that increase inflammation and cause periodontal tissue destruction.

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人巨噬细胞免疫代谢调节因子(MACIR)在牙周炎患者中的作用

目的牙周炎是一种由细菌感染引起的局部炎症反应，包括巨噬细胞在内的免疫细胞参与其中。近年来的研究表明，巨噬细胞功能的一个重要调节因子是人巨噬细胞免疫代谢调节因子(MACIR)。该基因已被证明通过影响成纤维细胞和巨噬细胞的活性在调节免疫反应中发挥关键作用。本研究研究了牙周病患者牙龈组织中MACIR的表达，以及IL-1β和TNF-α对人牙龈成纤维细胞中MACIR基因和蛋白表达的影响。方法采用Real-time PCR检测牙龈组织中smacir mRNA的表达。免疫荧光染色和western blotting检测MACIR蛋白的表达。结果牙周炎患者牙龈组织标本中MACIR mRNA表达量低于健康对照组，差异有统计学意义(p = 0.009)。IL-1β和TNF-α刺激人牙龈成纤维细胞后，MACIR基因mRNA表达显著降低。western blotting和免疫荧光分析证实，IL-1β和TNF-α刺激人牙龈成纤维细胞原代培养可降低MACIR蛋白的表达。结论MACIR是牙周炎患者炎症过程的重要调节因子。MACIR基因表达的降低可能激活巨噬细胞分泌增加炎症和导致牙周组织破坏的介质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunobiology 医学-免疫学

CiteScore

5.00

自引率

3.60%

发文量

108

审稿时长

55 days

期刊介绍： Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.