Improved methods for the determination of 5-bromo-2-deoxyuridine labelling index in clones and colonies growing in plasma clots.

Basic and applied histochemistry Pub Date : 1990-01-01
G D Di Nucci, P L Tazzari, A Bontadini, C Tassi, A Dinota, S Pileri, M Gobbi
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Abstract

Bromodeoxyuridine (BrdUrd), an analogue of thymidine is one of the most employed marker to detect the S-phase of the cell cycle. Difficulties are described for the in situ detection of S-phase cells in normal and neoplastic growing clones. In this paper we propose new methods for the detection of BrdUrd in neoplastic clones, growing in plasma clots. In particular, these methods are based on the immunocytochemical staining with horseradish peroxidase and alkaline phosphatase, as well as on a new immunofluorescent streptavidin technique. They allow easy detection of S-phase cells with an inverted light microscope.

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血浆凝块中5-溴-2-脱氧尿苷标记指数测定方法的改进。
溴脱氧尿嘧啶(BrdUrd)是胸苷的类似物,是检测细胞周期s期最常用的标记物之一。描述了在正常和肿瘤生长克隆中原位检测s期细胞的困难。本文提出了在血浆凝块中生长的肿瘤克隆中检测BrdUrd的新方法。特别是,这些方法是基于辣根过氧化物酶和碱性磷酸酶的免疫细胞化学染色,以及一种新的免疫荧光链霉亲和素技术。它们可以很容易地用倒置光学显微镜检测s期细胞。
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