Artificial bacterial consortium for bioremediation of hydrocarbon polluted sites

Mouna Mahjoubi, Simone Cappello, Yasmine Souissi, Sabrina Patania, Ameur Cherif
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Abstract

AbstractBioremediation is one of the most effective strategies to reduce pollution of oil contaminated environments. In this study, the ability of selected bacterial strains to degrade crude oil and its components was evaluated through growth experiments and gas chromatographic analysis. The strains were taxonomically characterized as Alcanivorax, Labrenzia, Halomonas, Acinetobacter, Alcaligenes, Pseudomonas and Brevundimonas. Four different bacterial consortia were constructed and their biodegradation capabilities were evaluated. One of them showing high degradation capability of aliphatic and polycyclic aromatic hydrocarbons has been used for bioaugmentation in a microcosm for 30 days, and its bioremediation potential showed significant reduction of hydrocarbons in contaminated sediments.Keywords: Hydrocarbons degrading bacteriabiodegradationmicrobial consortiaallochthonous bioaugmentationbioremediationDisclaimerAs a service to authors and researchers we are providing this version of an accepted manuscript (AM). Copyediting, typesetting, and review of the resulting proofs will be undertaken on this manuscript before final publication of the Version of Record (VoR). During production and pre-press, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal relate to these versions also. AcknowledgmentsWe thank all of the members in our academic group for helping us to complete the experiments
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烃类污染场地生物修复的人工细菌联合体
摘要生物修复是减少石油污染环境污染的最有效策略之一。在本研究中,通过生长实验和气相色谱分析,评价了选定菌株对原油及其组分的降解能力。菌株分类为Alcanivorax、Labrenzia、Halomonas、Acinetobacter、Alcaligenes、Pseudomonas和Brevundimonas。构建了4个不同的菌群,并对其生物降解能力进行了评价。其中一种对脂肪族和多环芳烃具有较强降解能力的生物增强剂在微环境中进行了30天的生物修复,其生物修复潜力显示出污染沉积物中碳氢化合物的显著减少。关键词:碳氢化合物降解细菌降解微生物群落异体生物增强生物修复免责声明作为对作者和研究人员的服务,我们提供这个版本的接受手稿(AM)。在最终出版版本记录(VoR)之前,将对该手稿进行编辑、排版和审查。在制作和印前,可能会发现可能影响内容的错误,所有适用于期刊的法律免责声明也与这些版本有关。感谢我们学术小组的所有成员帮助我们完成实验
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