The Triterpenoid High-Performance Liquid Chromatography Analytical Profiles of the Mycelia of Ganoderma lucidum (lingzhi)

IF 2.1 Q3 MICROBIOLOGY Microbiology Research Pub Date : 2023-09-14 DOI:10.3390/microbiolres14030092

Deng-Hai Chen, Jian-Yuan Wang, Mon-Tarng Chen, Yen-Chun Liu, Kuang-Dee Chen

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Abstract

(1) Background: Ganoderic acids (GAs) are specific triterpenes of Ganoderma lucidum. The HPLC fingerprint profile of GAs of the fruiting body is well known, but their mycelial fingerprinting remains unclear. (2) Methods: An ethanol extract of the mycelium of G. lucidum (YK-01) was further purified via preparative HPLC. The triterpenoid compositions for four strains of G. lucidum and one strain of G. formosanum (purple lingzhi) were analyzed using HPLC. (3) Results: Nineteen lanostane triterpenes, including five new triterpenes, GA-TP (1), ganodermic acid Jc (GmA-Jc) (2), GmA-Jd (3), GA-TQ1 (4), and ganoderal B1 (5), and fourteen known triterpenes 6–19 were isolated from the ethanol extract. Their structures were identified by mass and extensive NMR spectroscopy. A green chemical HPLC analytical method was developed using ethanol and acetic acid as a mobile phase, and all isolated compounds can be well separated. These triterpenes comprise a unique HPLC chromatograph of the G. lucidum mycelium. All four G. lucidum strains showed the same HPLC chromatographic pattern, whereas G. formosanum displayed a different pattern. Quantitation methods for ganoderic acid T (10) and S (12) were also validated. (4) Conclusions: The triterpenoid HPLC analytical method can be used to identify the G. lucidum species and to determine the contents of GA-T and GA-S.

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灵芝菌丝体三萜高效液相色谱分析谱

(1)背景:灵芝酸(Ganoderic acids, GAs)是灵芝特有的三萜。子实体GAs的HPLC指纹图谱是已知的，但其菌丝体指纹图谱尚不清楚。(2)方法:采用制备高效液相色谱法对灵芝菌丝体(YK-01)乙醇提取物进行进一步纯化。采用高效液相色谱法对4株灵芝和1株台湾灵芝的三萜成分进行了分析。(3)结果:从乙醇提取物中分离得到19种毛甾三萜，包括5种新三萜，GA-TP(1)、灵芝酸Jc (GmA-Jc)(2)、GmA-Jd(3)、GA-TQ1(4)和灵芝B1(5)，以及14种已知三萜6-19。它们的结构通过质谱和广泛的核磁共振谱进行了鉴定。建立了以乙醇和乙酸为流动相的绿色化学高效液相色谱分析方法，分离得到的化合物均能很好地分离。这些三萜组成了一种独特的高效液相色谱色谱的灵芝菌丝体。4株灵芝均表现出相同的HPLC图谱，而台湾灵芝则表现出不同的图谱。同时验证了灵芝酸T(10)和S(12)的定量方法。(4)结论:三萜高效液相色谱法可用于鉴别灵芝的种类，测定灵芝中GA-T和GA-S的含量。

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来源期刊

Microbiology Research MICROBIOLOGY-

CiteScore

1.90

自引率

6.70%

发文量

审稿时长

10 weeks

期刊介绍： Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.