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Updates on Staphylococcal Vaccines 葡萄球菌疫苗的最新进展
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-28 DOI: 10.3390/microbiolres15010009
Alexandru Scafa-Udriste, M. Popa, G. Popa
Staphylococcus aureus, a prevalent human pathogen and a leading cause of hospital-acquired infections, is increasingly evolving antibiotic-resistant strains, increasing mortality and morbidity rates. Anti-staphylococcal vaccine research for prevention and treatment has become a priority. Antibodies against specific S. aureus components, toxins, and polysaccharides have demonstrated encouraging results in animal studies regarding protection against colonization or infection. However, human immunization trials have yielded less optimistic outcomes, with no anti-staphylococcal having passed clinical trials up to now. Although multiple formulation attempts triggered strong antibody responses, the vaccines could not effectively prevent S. aureus infections. This article delves into the results of immunotherapeutic strategies against S. aureus in both animal and human studies, discussing the feasibility of adequate immunization approaches against S. aureus in humans.
金黄色葡萄球菌是一种常见的人类病原体,也是医院获得性感染的主要病因,其抗生素耐药菌株正在不断进化,从而增加了死亡率和发病率。用于预防和治疗的抗葡萄球菌疫苗研究已成为当务之急。针对特定金黄色葡萄球菌成分、毒素和多糖的抗体已在动物实验中显示出令人鼓舞的保护作用,可防止定植或感染。然而,人类免疫试验的结果并不乐观,迄今为止还没有一种抗葡萄球菌药物通过临床试验。虽然多种配方的尝试引发了强烈的抗体反应,但这些疫苗无法有效预防金黄色葡萄球菌感染。本文深入探讨了针对金黄色葡萄球菌的免疫治疗策略在动物和人体研究中的结果,讨论了针对金黄色葡萄球菌的适当免疫方法在人体中的可行性。
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引用次数: 0
Assess the Diagnostic Accuracy of GeneXpert to Detect Mycobacterium tuberculosis and Rifampicin-Resistant Tuberculosis among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients 评估 GeneXpert 在推定肺结核和推定耐药肺结核患者中检测结核分枝杆菌和耐利福平结核病的诊断准确性
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-22 DOI: 10.3390/microbiolres15010006
Venkateswari Ramachandra, Usharani Brammacharry, Aaina Muralidhar, Anbazhagi Muthukumar, Revathi Mani, M. Muthaiah, Govindarajan Soundappan, Asha Frederick
GeneXpert MTB/RIF is a rapid molecular diagnostic tool capable of simultaneously detecting Mycobacterium tuberculosis and rifampicin resistance. This study aimed to assess the diagnostic precision of GeneXpert MTB/RIF assay to detect pulmonary and extrapulmonary tuberculosis and evaluate the performance for detecting of rifampicin resistance. Of 37,695 samples, 7156 (18.98%) were tuberculosis-positive, and 509 (7.11%) were rifampicin-resistant. The sensitivity, specificity, positive predictive value, negative predictive value, disease prevalence, and accuracy of the GeneXpert MTB/RIF assay for pulmonary tuberculosis were 99.87% (95%CI: 99.75–99.94), 99.92% (95%CI: 99.88–99.95), 99.71% (95%CI: 99.54–99.82), 99.97% (95%CI: 99.93–99.98), 21.38% (95%CI: 20.92–21.86), and 99.91% (95%CI: 99.87–99.94), respectively. For extrapulmonary tuberculosis, the sensitivity, specificity, PPV, NPV, disease prevalence, and accuracy of GeneXpert MTB/RIF assay accounted for 99.45% (95%CI: 98.73–99.82), 99.84% (95%CI: 99.73–99.92), 98.70% (95%CI: 97.73–99.25), 99.93% (95%CI: 99.84–99.97), 10.64% (95%CI: 9.99–11.31), and 99.80% (95%CI: 99.68–99.88), respectively. Despite its high sensitivity for detecting tuberculosis and rifampicin resistance, GeneXpert MTB/RIF had contradictory results for 20.5% of cases among patients with smear-negative results and 54.9% of cases among patients with a high risk of multidrug-resistant tuberculosis. Of 46% fluoroquinolone-resistant cases, 16.56% (26/157) were multidrug-resistant tuberculosis isolates, and 4.02% (20/498) were isoniazid-resistant, a characteristic distribution leading to about 17.2% of fluoroquinolone-resistance events and relevant marker gyr-A mutations in MDR tuberculosis isolates. Further, our study indicated that increased fluoroquinolone resistance among rifampicin-resistant and isoniazid-resistant tuberculosis endangers the success of newly endorsed MDR-TB regimens.
GeneXpert MTB/RIF 是一种快速分子诊断工具,能够同时检测结核分枝杆菌和利福平耐药性。本研究旨在评估 GeneXpert MTB/RIF 检测法在检测肺结核和肺外结核病方面的诊断精确度,并评估其在检测利福平耐药性方面的性能。在 37,695 份样本中,7156 份(18.98%)结核菌阳性,509 份(7.11%)对利福平耐药。GeneXpert MTB/RIF 检测法对肺结核的灵敏度、特异性、阳性预测值、阴性预测值、疾病流行率和准确性分别为 99.87% (95%CI: 99. 75-99.94), 99.75-99.94)、99.92%(95%CI:99.88-99.95)、99.71%(95%CI:99.54-99.82)、99.97%(95%CI:99.93-99.98)、21.38%(95%CI:20.92-21.86)和 99.91%(95%CI:99.87-99.94)。对于肺外结核病,GeneXpert MTB/RIF 检测的敏感性、特异性、PPV、NPV、疾病流行率和准确性分别为 99.45%(95%CI:98.73-99.82)、99.84%(95%CI:99.87-99.94)和 99.91%(95%CI:99.87-99.94)。82)、99.84%(95%CI:99.73-99.92)、98.70%(95%CI:97.73-99.25)、99.93%(95%CI:99.84-99.97)、10.64%(95%CI:9.99-11.31)和 99.80%(95%CI:99.68-99.88)。尽管 GeneXpert MTB/RIF 检测结核病和利福平耐药性的灵敏度很高,但在涂片结果阴性的患者中,20.5% 的病例和 54.9% 的耐多药结核病高危患者的检测结果存在矛盾。在46%的耐氟喹诺酮病例中,16.56%(26/157)为耐多药结核病分离株,4.02%(20/498)为耐异烟肼结核病分离株,这种特征性分布导致约17.2%的耐氟喹诺酮事件和MDR结核病分离株中的相关标记物gyr-A突变。此外,我们的研究还表明,耐利福平和耐异烟肼结核病患者对氟喹诺酮类药物耐药性的增加会危及新批准的 MDR-TB 治疗方案的成功。
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引用次数: 0
Biodegradation of Free Cyanide by a New Isolated Alkaliphilic Bacillus licheniformis Strain 地衣芽孢杆菌新菌株对游离氰化物的生物降解
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/microbiolres15010003
Daniel Uribe-Ramírez, E. Cristiani-Urbina, L. Morales-Barrera
Microbial treatment of free-cyanide-polluted wastewater is a cost-effective, efficient, and eco-friendly method. Free-cyanide-degrading microbial cultures were isolated from different sources using batch-enrichment culture techniques, with acetate as the carbon source. Five microbial cultures were able to tolerate and grow at 1500 mg/L free cyanide, which was used as the only nitrogen source under strongly alkaline conditions (pH = 11). Among them, one bacterial strain (B11) was selected for further study because of its high free-cyanide-biodegradation efficiency. Bacterial strain B11 was molecularly identified as Bacillus licheniformis CDBB B11. Free cyanide inhibited the growth rate of B. licheniformis CDBB B11 at initial cyanide concentrations >75 mg/L. Despite this, the bacterial strain demonstrated 100% cyanide-biodegradation efficiency at initial cyanide concentrations ranging from 25 to 75 mg/L, which decreased to 32% as the initial cyanide concentration increased from 75 to 1500 mg/L. Free-cyanide biodegradation corresponds to bacterial growth and ammonia accumulation in the culture medium. The alkaliphilic B. licheniformis CDBB B11 strain is a robust candidate for the detoxification of free-cyanide-laden wastewater because it tolerates and efficiently degrades free cyanide at concentrations of up to 1500 mg/L.
用微生物处理游离氰化物污染的废水是一种经济、高效和环保的方法。利用批量富集培养技术,以醋酸盐为碳源,从不同来源分离出了能降解游离氰化物的微生物培养物。在强碱性条件(pH = 11)下,以 1500 mg/L 游离氰化物为唯一氮源,有五种微生物培养物能够耐受并生长。其中,一个细菌菌株(B11)因其较高的游离氰生物降解效率而被选中作进一步研究。菌株 B11 经分子鉴定为地衣芽孢杆菌 CDBB B11。当初始氰化物浓度大于 75 毫克/升时,游离氰化物会抑制地衣芽孢杆菌 CDBB B11 的生长速度。尽管如此,在初始氰化物浓度为 25 至 75 毫克/升时,该细菌菌株的氰化物生物降解效率为 100%,而当初始氰化物浓度从 75 毫克/升增加到 1500 毫克/升时,生物降解效率下降到 32%。游离氰化物的生物降解与培养基中的细菌生长和氨积累相对应。嗜碱性地衣芽孢杆菌 CDBB B11 菌株可耐受并高效降解浓度高达 1500 毫克/升的游离氰,因此是含游离氰废水解毒的理想候选菌株。
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引用次数: 0
Genome Sequence and Characterisation of Peribacillus sp. Strain AS_2, a Bacterial Endophyte Isolated from Alectra sessiliflora Peribacillus sp.菌株 AS_2 的基因组序列和特征,它是一种从 Alectra sessiliflora 分离出来的内生细菌
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/microbiolres15010004
M. P. Maela, M. H. Serepa-Dlamini
Peribacillus sp. AS_2, a leaf endophytic bacterium isolated from the medicinal plant Alectra sessiliflora, was previously identified using the 16S rRNA gene sequence. The draft genome of AS_2 had a 5,482,853 bp draft circular chromosome, 43 contigs, N50 of 360,633 bp and an average G + C% content of 40.5%. Whole genome sequencing and phenotypic analysis showed that AS_2 was Gram-positive, endospore-forming, motile, and rod-shaped and it showed a high sequence similarity with P. frigoritolerans DSM 8801T. Genomic digital DNA–DNA hybridisation (dDDH) between strain AS_2 and Peribacillus frigoritolerans DSM 8801T and P. castrilensis N3T was 84.8% and 79.2%, respectively, and the average nucleotide identity (ANI) of strain AS_2 with P. frigoritolerans DSM 8801T and P. castrilensis N3T was 97.0% and 96.7%, respectively. The antiSMASH software predicted a total of eight secondary metabolite gene clusters comprising non-ribosomal peptide synthetase (NRPS) type koranimine, terpenes, and siderophore clusters. Strain AS_2 also displayed genes involved in endophytic lifestyle and antibiotic resistance gene clusters such as small multidrug resistance antibiotic efflux pumps (qacJs). Using the multilocus sequence analysis (MLSA), together with the phenotypic data and genomic analysis, we demonstrated that strain AS_2 is a subspecies of P. frigoritolerans DSM 8801T. Genome sequencing of Peribacillus sp. AS_2 from medicinal plants provides valuable genomic information and allows us to further explore its biotechnological applications.
先前利用 16S rRNA 基因序列鉴定了从药用植物 Alectra sessiliflora 分离出的叶内生细菌 Peribacillus sp.AS_2 的基因组草案有一个 5,482,853 bp 的环状染色体草案,43 个等位基因,N50 为 360,633 bp,平均 G + C% 含量为 40.5%。全基因组测序和表型分析表明,AS_2呈革兰氏阳性,有内生孢子,能运动,呈杆状,与P. frigoritolerans DSM 8801T的序列高度相似。AS_2菌株与Peribacillus frigoritolerans DSM 8801T和P. castrilensis N3T的基因组数字DNA-DNA杂交(dDDH)分别为84.8%和79.2%,AS_2菌株与P. frigoritolerans DSM 8801T和P. castrilensis N3T的平均核苷酸同一性(ANI)分别为97.0%和96.7%。反SMASH软件共预测了8个次级代谢物基因簇,包括非核糖体肽合成酶(NRPS)型柯拉明、萜类和苷元簇。菌株 AS_2 还显示了参与内生生活方式的基因和抗生素抗性基因簇,如小型多药抗性抗生素外排泵(qacJs)。利用多焦点序列分析(MLSA)、表型数据和基因组分析,我们证明了菌株 AS_2 是 P. frigoritolerans DSM 8801T 的一个亚种。对药用植物中的 Peribacillus sp.
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引用次数: 0
Bioactive Diepoxy Metabolites and Highly Oxygenated Triterpenoids from Marine and Plant-Derived Bacteria and Fungi 海洋和植物细菌及真菌中具有生物活性的二环氧代谢物和高含氧三萜类化合物
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-21 DOI: 10.3390/microbiolres15010005
Valery M Dembitsky
This article explores the diverse array of biologically active compounds derived from microbial symbionts, particularly focusing on the isolation and characterization of diepoxides, highly oxygenated triterpenoids, secosteroids, ergostane-type steroids, and meroterpenoids from various marine and plant-derived fungi. We highlight significant discoveries such as vitamin D variants from fungal species, unique sesterterpenoids from mangrove endophytic fungi, and secosteroids with potential medicinal applications. The study delves into the structural uniqueness and bioactivities of these compounds, including their anti-inflammatory, antibacterial, antifungal, antiviral, and cytotoxic effects. Notable findings include the isolation of compounds with significant activity against cancer cell lines, the inhibition of acetylcholinesterase, and promising antifouling properties. This work underscores the potential of microbial symbionts as a rich source of novel bioactive compounds with diverse therapeutic applications, highlighting the importance of marine and fungal biodiversity in drug discovery and development.
本文探讨了从微生物共生体中提取的各种生物活性化合物,尤其侧重于从各种海洋真菌和植物源真菌中分离和鉴定二环氧化物、高含氧三萜类化合物、甾体类化合物、麦角甾体类化合物和经络萜类化合物。我们重点介绍了一些重大发现,如真菌物种中的维生素 D 变体、红树林内生真菌中独特的酯萜类化合物以及具有潜在药用价值的类固醇。研究深入探讨了这些化合物的结构独特性和生物活性,包括它们的抗炎、抗菌、抗真菌、抗病毒和细胞毒性作用。值得注意的发现包括分离出对癌细胞株有显著活性的化合物、抑制乙酰胆碱酯酶的化合物以及具有良好防污特性的化合物。这项工作强调了微生物共生体作为具有多种治疗用途的新型生物活性化合物的丰富来源的潜力,突出了海洋和真菌生物多样性在药物发现和开发中的重要性。
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引用次数: 0
Properties of Potential Plant-Growth-Promoting Bacteria and Their Effect on Wheat Growth Promotion (Triticum aestivum) and Soil Characteristics 潜在植物生长促进细菌的特性及其对小麦生长促进(Triticum aestivum)和土壤特性的影响
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-19 DOI: 10.3390/microbiolres15010002
E. Voronina, Ekaterina Sokolova, Irina Tromenschleger, Olga Mishukova, Inna Hlistun, Matvei Miroshnik, Oleg Savenkov, Maria Buyanova, Ilya Ivanov, Maria R. Galyamova, Natalya Smirnova
Plant-growth-promoting bacteria are an important economic and environmental resource as biofertilizers that can stimulate plant growth and improve agricultural yields. In this study, potential plant growth-promoting bacteria were isolated from soil samples collected in Russia. Strains that manifested active growth on a nitrogen-free medium, the Pikovskaya medium (with insoluble phosphates) and CAS (Chrome Azurol S) agar, were selected for the study. All bacterial isolates were identified by 16S rRNA gene sequencing analysis. Seventeen bacterial isolates of different species were purified and quantified for their ability to grow on nitrogen-free media; dissolve phosphate; and produce ammonium, indole-3-acetic acid, siderophores, and antifungicidal agents. Principal component analysis identified three groups of strains: one with the maximum signs of providing “plant nutrition”; one with signs of “antimicrobial activity”; and a group “without outstanding signs”. All 17 strains were involved in experiments involving growing inoculated wheat seeds (Triticum aestivum) in pots under natural environmental conditions, and were assessed by their effect on the wheat growth and yield as well as on the chemical composition of the soil. For the “plant nutrition” group, regression analysis revealed a connection between indicators of plant growth, ear length, and ammonium accumulation in the soil. However, in other groups, there were also strains showing a positive effect on plant growth, which suggests the necessity of involving additional factors to predict the ability of strains to affect plants when screened in the laboratory.
植物生长促进细菌是一种重要的经济和环境资源,可作为生物肥料刺激植物生长,提高农业产量。本研究从俄罗斯采集的土壤样本中分离出了潜在的植物生长促进细菌。研究选择了在无氮培养基、Pikovskaya 培养基(含不溶性磷酸盐)和 CAS(Chrome Azurol S)琼脂上生长活跃的菌株。所有细菌分离物都通过 16S rRNA 基因测序分析进行了鉴定。纯化了 17 个不同种类的细菌分离物,并对其在无氮培养基上的生长能力、溶解磷酸盐的能力、产生铵、吲哚-3-乙酸、嗜苷酸和抗真菌剂的能力进行了量化。主成分分析确定了三组菌株:一组具有提供 "植物营养 "的最大迹象;一组具有 "抗菌活性 "迹象;还有一组 "无突出迹象"。所有 17 种菌株都参与了在自然环境条件下盆栽接种小麦种子(Triticum aestivum)的实验,并通过它们对小麦生长和产量以及土壤化学成分的影响进行了评估。就 "植物营养 "组而言,回归分析表明,植物生长指标、穗长和土壤中的铵积累之间存在联系。不过,在其他组别中,也有一些菌株对植物生长有积极影响,这表明在实验室筛选菌株时,有必要考虑其他因素来预测菌株对植物的影响能力。
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引用次数: 0
Enhancing Understanding of the Impact of Climate Change on Malaria in West Africa Using the Vector-Borne Disease Community Model of the International Center for Theoretical Physics (VECTRI) and the Bias-Corrected Phase 6 Coupled Model Intercomparison Project Data (CMIP6) 利用国际理论物理中心(VECTRI)的病媒传染病群落模型和经偏差校正的第 6 阶段耦合模型相互比较项目数据(CMIP6),加深理解气候变化对西非疟疾的影响
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-18 DOI: 10.3390/microbiolres14040145
Papa Fall, Ibrahima Diouf, A. Deme, Sémou Diouf, D. Sène, Benjamin Sultan, Serge Janicot
In sub-Saharan Africa, temperatures are generally conducive to malaria transmission, and rainfall provides mosquitoes with optimal breeding conditions. The objective of this study is to assess the impact of future climate change on malaria transmission in West Africa using community-based vector-borne disease models, TRIeste (VECTRI). This VECTRI model, based on bias-corrected data from the Phase 6 Coupled Model Intercomparison Project (CMIP6), was used to simulate malaria parameters, such as the entomological inoculation rate (EIR). Due to the lack of data on confirmed malaria cases throughout West Africa, we first validated the forced VECTRI model with CMIP6 data in Senegal. This comparative study between observed malaria data from the National Malaria Control Program in Senegal (Programme National de Lutte contre le Paludisme, PNLP, PNLP) and malaria simulation data with the VECTRI (EIR) model has shown the ability of the biological model to simulate malaria transmission in Senegal. We then used the VECTRI model to reproduce the historical characteristics of malaria in West Africa and quantify the projected changes with two Shared Socio-economic Pathways (SSPs). The method adopted consists of first studying the climate in West Africa for a historical period (1950–2014), then evaluating the performance of VECTRI to simulate malaria over the same period (1950–2014), and finally studying the impact of projected climate change on malaria in a future period (2015–2100) according to the ssp245 ssp585 scenario. The results showed that low-latitude (southern) regions with abundant rainfall are the areas most affected by malaria transmission. Two transmission peaks are observed in June and October, with a period of high transmission extending from May to November. In contrast to regions with high latitudes in the north, semi-arid zones experience a relatively brief transmission period that occurs between August, September, and October, with the peak observed in September. Regarding projections based on the ssp585 scenario, the results indicate that, in general, malaria prevalence will gradually decrease in West Africa in the distant future. But the period of high transmission will tend to expand in the future. In addition, the shift of malaria prevalence from already affected areas to more suitable areas due to climate change is observed. Similar results were also observed with the ssp245 scenario regarding the projection of malaria prevalence. In contrast, the ssp245 scenario predicts an increase in malaria prevalence in the distant future, while the ssp585 scenario predicts a decrease. These findings are valuable for decision makers in developing public health initiatives in West Africa to mitigate the impact of this disease in the region in the context of climate change.
在撒哈拉以南非洲地区,气温通常有利于疟疾的传播,而降雨则为蚊子提供了最佳的繁殖条件。这项研究的目的是利用基于社区的病媒传播疾病模型 TRIeste(VECTRI),评估未来气候变化对西非疟疾传播的影响。该 VECTRI 模型基于第六阶段耦合模型相互比较项目(CMIP6)的偏差校正数据,用于模拟疟疾参数,如昆虫接种率(EIR)。由于缺乏整个西非的疟疾确诊病例数据,我们首先在塞内加尔利用 CMIP6 数据验证了 VECTRI 强制模型。塞内加尔国家疟疾控制项目(PNLP)的疟疾观测数据与 VECTRI(EIR)模型的疟疾模拟数据之间的比较研究表明,生物模型有能力模拟塞内加尔的疟疾传播情况。随后,我们利用 VECTRI 模型再现了西非疟疾的历史特征,并量化了两种共享社会经济路径(SSP)的预测变化。所采用的方法包括:首先研究西非历史时期(1950-2014 年)的气候,然后评估 VECTRI 模拟同一时期(1950-2014 年)疟疾的性能,最后根据 ssp245 ssp585 情景研究预测气候变化对未来时期(2015-2100 年)疟疾的影响。结果表明,降雨丰富的低纬度(南部)地区是疟疾传播最严重的地区。6 月和 10 月出现两个传播高峰,5 月至 11 月为高传播期。与北部高纬度地区相比,半干旱地区的传播期相对较短,在 8 月、9 月和 10 月之间,传播高峰期出现在 9 月。关于基于 ssp585 情景的预测,结果表明,总体而言,在遥远的未来,西非的疟疾流行率将逐渐下降。但高传播期在未来将有扩大的趋势。此外,由于气候变化,疟疾流行将从已受影响的地区转移到更合适的地区。在 ssp245 情景下,对疟疾流行率的预测也出现了类似的结果。相比之下,sp245 情景预测在遥远的未来疟疾流行率会上升,而 ssp585 情景则预测会下降。这些发现对决策者在气候变化背景下制定西非公共卫生倡议以减轻该地区疟疾影响很有价值。
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引用次数: 0
Novel Vesicular Formulation Based on a Herbal Extract Loaded with Niosomes and Evaluation of Its Antimicrobial and Anticancer Potential 基于含有 Niosomes 的草药提取物的新型囊泡制剂及其抗菌和抗癌潜力评估
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-16 DOI: 10.3390/microbiolres14040144
N. Al-Enazi, Khawla E. Alsamhary, F. Ameen, Marcos Augusto Lima Nobre
This study aimed to enhance the anticancer and antibacterial properties of Pistacia atlantica through a new parenteral formulation. The innovative approach involved loading P. atlantica fruit extract onto a novel Pluronic vesicular nano platform (Nio), analyzed using various techniques like GC-Mass, SEM, DLS, and UV-vis. The results revealed a stable, spherical Nio/Extract formulation of 103 ± 4.1 nm, possessing a high zeta potential of −30 ± 2.3 mV, with an impressive encapsulation efficiency of nearly 90 ± 3.5%. This formulation exhibited heightened cytotoxicity against BT-20 and MCF-10 cell lines compared to the extract alone, indicating its potential as a drug carrier with prolonged release characteristics. Additionally, the Nio/Extract demonstrated superior antibacterial effects against Pseudomonas aeruginosa, Staphylococcus epidermidis, and Candida albicans compared to the free extract, showcasing MIC values of 211, 147, and 187 ug/mL, respectively, versus 880, 920, and 960 ug/mL for the pathogens. These findings highlight the potential of niosomal nano-carriers housing P. atlantica extract as a viable therapeutic strategy for combating both malignancies and microbial infections.
本研究旨在通过一种新的肠外制剂来增强Pistacia atlantica 的抗癌和抗菌特性。创新方法包括将桔梗果实提取物装载到新型 Pluronic 微囊纳米平台(Nio)上,并使用 GC-Mass、SEM、DLS 和 UV-vis 等多种技术进行分析。结果显示,Nio/提取物制剂呈稳定的球形,直径为 103 ± 4.1 nm,zeta 电位高达 -30 ± 2.3 mV,封装效率接近 90 ± 3.5%,令人印象深刻。与单独的提取物相比,该制剂对 BT-20 和 MCF-10 细胞株的细胞毒性更强,这表明它有潜力成为一种具有长效释放特性的药物载体。此外,与游离提取物相比,Nio/提取物对铜绿假单胞菌、表皮葡萄球菌和白色念珠菌的抗菌效果更佳,其 MIC 值分别为 211、147 和 187 微克/毫升,而对病原体的 MIC 值分别为 880、920 和 960 微克/毫升。这些研究结果凸显了纳米载体(niosomal nano-carrier housing P. atlantica extract)作为抗击恶性肿瘤和微生物感染的可行治疗策略的潜力。
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引用次数: 0
Fungal Endophytes of Moringa (Moringa oleifera L.), Neem (Azadirachta indica) and Lavender (Lavandula angustifolia) and Their Biological Control of Fusarium Wilt of Banana 辣木(Moringa oleifera L.)、印楝(Azadirachta indica)和薰衣草(Lavandula angustifolia)的真菌内生菌及其对香蕉镰刀菌枯萎病的生物防治作用
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-15 DOI: 10.3390/microbiolres14040143
Bonface M. Nthuku, Esther W. Kahariri, Johnson K. Kinyua, Evans N. Nyaboga
Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), significantly affects the productivity of the banana crop in the field. Currently, there are no effective control measures available, and therefore, there is an urgent need to develop novel strategies to control the spread of the disease. Biological control is a promising strategy for the management and control of the disease. The aim of this study was to identify fungal endophytes associated with Moringa (Moringa oleifera), Neem tree (Azadirachta indica) and Lavender (Lavandula angustifolia) and their antifungal activities against Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). We isolated 69 fungal endophytes from different tissues of M. oleifera, A. indica and L. angustifolia and screened for antifungal activity against Foc TR4. Six fungal endophytes exhibited highest antagonistic activity against Foc TR4 based on dual-culture assays. Based on morphology and ITS gene sequence analysis, the selected six endophytes were identified to be related to Alternaria alternata (MB7 and NR3), Neofusicoccum parvum (LB1), Fusarium oxysporum (LR1), Talaromyces amestolkiae (MB14) and Alternaria tenuissima (NB6). The culture filtrates of the six fungal endophytes (LB1, LR1, MB7, MB14, NB6 and NR3) exhibited more than 50% inhibition of mycelial growth of Foc TR4 in vitro and were producers of β-1,3-glucanase. The six fungal endophytes showed biocontrol efficacy against Fusarium wilt in pot experiments. The findings from this study demonstrate that fungal endophytes LB1, LR1, MB7, MB14, NB6 and NR3 should be explored as biocontrol agents and biofertilizers in banana production.
由 Fusarium oxysporum f. sp. cubense(Foc)引起的镰刀菌枯萎病严重影响了香蕉作物的产量。目前还没有有效的控制措施,因此迫切需要开发新的战略来控制该疾病的传播。生物防治是管理和控制该疾病的一种有前途的策略。本研究的目的是鉴定与辣木(Moringa oleifera)、印度楝树(Azadirachta indica)和薰衣草(Lavandula angustifolia)相关的真菌内生菌,以及这些真菌内生菌对 Fusarium oxysporum f. sp. cubense tropical race 4(Foc TR4)的抗真菌活性。我们从 M.oleifera、A. indica 和 L. angustifolia 的不同组织中分离出 69 种真菌内生菌,并筛选出它们对 Foc TR4 的抗真菌活性。根据双重培养试验,6 种真菌内生菌对 Foc TR4 的拮抗活性最高。根据形态学和 ITS 基因序列分析,所选的六种内生真菌被鉴定为与交替丝核菌(MB7 和 NR3)、Neofusicoccum parvum(LB1)、Fusarium oxysporum(LR1)、Talaromyces amestolkiae(MB14)和 Alternaria tenuissima(NB6)有关。六种真菌内生菌(LB1、LR1、MB7、MB14、NB6 和 NR3)的培养滤液在体外对 Foc TR4 菌丝生长的抑制率超过 50%,并能产生 β-1,3-葡聚糖酶。在盆栽实验中,这六种真菌内生菌对镰刀菌枯萎病具有生物防治效果。这项研究结果表明,真菌内生菌 LB1、LR1、MB7、MB14、NB6 和 NR3 应作为生物防治剂和生物肥料在香蕉生产中进行开发。
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引用次数: 0
Assessment of the Impacts of Plant Growth-Promoting Micro-Organisms on Potato Farming in Different Climatic Conditions in Morocco 摩洛哥不同气候条件下促进植物生长的微生物对马铃薯种植的影响评估
IF 1.5 Q3 MICROBIOLOGY Pub Date : 2023-12-09 DOI: 10.3390/microbiolres14040141
Nadia El Allaoui, Hiba Yahyaoui, A. Douira, A. Benbouazza, Moha Ferrahi, E. Achbani, K. Habbadi
Environmental concerns are gradually reducing the global yield capacity of agricultural systems, with climate change representing the most significant challenge. Globally, Potatoes are the most essential non-cereal crop. Therefore, understanding the potential impacts of climate change on potato production is crucial for maintaining future global food security. This study aims to explore the roles played by PGPMs in two distinct regions, which are characterized by different climatic conditions, to assess their influence on two potato varieties, namely Siena and Bellini. Inoculation with these strains, particularly the Aureobasidium pullulans strains Ach1-1 and Ach1-2, resulted in significant improvements in growth and yield. In 2018, impressive yields of 194.1 kg/0.05 ha and 186.6 kg/0.05 ha were recorded for the two strains, with the Ain Taoujdate site achieving yields of 157.1 kg/0.05 ha and 151.1 kg/0.05 ha for each of the two strains. Additionally, further observations revealed that the Siena variety is more susceptible to rot than the Bellini variety. However, Ach1-1 and Ach1-2 strains had a significant effect on this rot, showcasing their potential to mitigate this negative issue in the Bellini variety. These promising results underscore the potential of PGPMs to enhance potato production in the Fez–Meknes region of Morocco, contributing to global food security amid climate change.
环境问题正在逐渐削弱全球农业系统的产量能力,而气候变化则是最大的挑战。在全球范围内,马铃薯是最重要的非谷类作物。因此,了解气候变化对马铃薯生产的潜在影响对于维护未来全球粮食安全至关重要。本研究旨在探索 PGPM 在两个气候条件不同的地区所发挥的作用,以评估它们对两个马铃薯品种(锡耶纳和贝利尼)的影响。接种这些菌株,特别是Aureobasidium pullulans菌株Ach1-1和Ach1-2,可显著提高生长和产量。2018 年,这两种菌株的产量分别达到 194.1 千克/0.05 公顷和 186.6 千克/0.05 公顷,令人印象深刻,其中 Ain Taoujdate 基地的产量分别达到 157.1 千克/0.05 公顷和 151.1 千克/0.05 公顷。此外,进一步观察发现,锡耶纳品种比贝利尼品种更易感染腐烂病。然而,Ach1-1 和 Ach1-2 菌株对这种腐烂有显著影响,显示了它们减轻贝利尼品种这一负面问题的潜力。这些充满希望的结果突出表明,PGPMs 有潜力提高摩洛哥非斯-梅克内斯地区的马铃薯产量,从而在气候变化中为全球粮食安全做出贡献。
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引用次数: 0
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Microbiology Research
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