High-degree polymerizate IMOs of dextranase hydrolysates enhance Lactobacillus acid metabolism: Based on growth, and metabolomic and transcriptomic analyses

LWT Pub Date : 2023-09-01 DOI:10.1016/j.lwt.2023.115345

Qianru Lin, Mingwang Liu, Hao Ni, Yue Hao, Yiqun Yu, Yiran Chen, Qing Wu, Yi Shen, Lei Zhang, Mingsheng Lyu, Shujun Wang

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Abstract

This study aimed to explore the growth and metabolic mechanisms and functional properties of the combination of high-degree polymerizate (DP) isomaltooligosaccharides (IMOs) and Lactobacillus to provide a basis for their subsequent applications in functional foods in improving human health. Metabolomic and transcriptomic analyses were used to select the important metabolic pathways, regulatory genes, and metabolites involved in the growth of Lactobacillus by high DP IMOs. Lactobacillus paracasei JLPF-176 grew well in the MRS medium with high DP IMOs. The best effect in promoting L. paracasei JLPF-176 biofilm formation was 58.94%. When high DP IMOs hydrolyzed for 48 h were added to the MRS medium, 684 genes were upregulated and 276 genes were downregulated in L. paracasei JLPF-176, in important pathways such as d-amino acid metabolism, tryptophan metabolism, arginine and proline metabolism. HMDB compound classification statistics revealed 421 organic acids and derivatives, accounting for the highest number of 28.39%. The number of genes annotated in amino acid metabolism was 107.

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葡聚糖酶水解物的高度聚合IMOs增强乳酸杆菌的酸代谢:基于生长、代谢组学和转录组学分析

本研究旨在探讨高聚合(DP)寡聚异麦芽糖(IMOs)与乳酸菌的生长代谢机制及功能特性，为其在功能食品中的后续应用提供依据，以改善人类健康。通过代谢组学和转录组学分析，选择高DP IMOs对乳杆菌生长的重要代谢途径、调控基因和代谢物。副干酪乳杆菌JLPF-176在高DP IMOs的MRS培养基中生长良好。促进副淋球菌JLPF-176生物膜形成的最佳效果为58.94%。将水解48 h的高DP IMOs添加到MRS培养基中，在L. paracasei jlsf -176中，684个基因在d-氨基酸代谢、色氨酸代谢、精氨酸和脯氨酸代谢等重要通路上表达上调，276个基因表达下调。HMDB化合物分类统计显示有机酸及其衍生物421种，数量最多，占28.39%。氨基酸代谢注释基因数为107个。

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