CHITOSAN AND META-TOPOLIN SUPPLEMENTATION OF THE MULTIPLICATION MEDIA OF VITIS VINIFERA L.

IF 0.8 Q4 MATERIALS SCIENCE, BIOMATERIALS Progress on Chemistry and Application of Chitin and its Derivatives Pub Date : 2023-09-30 DOI:10.15259/pcacd.28.005

Marcelina Krupa-Małkiewicz, Ireneusz Ochmian

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Abstract

We investigated the efficiency of chitosan (CH) and meta-topolin (mT) on in vitro growth and adaptation to greenhouse conditions of Vitis vinifera cv. Johanniter and cv. Hibernal. After the initiation stage, we transferred explants to Woody Plant Media (WPM) with chitosan (molecular weight 3.33 kDa) at a concentration of 10, 20, and 40 ppm or to WPM with mT at a concentration of 0.5, 1.0, and 2.0 mg l–1. WPM without CH or mT served as a control. Among the tested combinations, WPM with 10 ppm of CH led to the maximum mean root length (12.18 cm and 12.65 cm for the Hibernal and Johanniter cultivars, respectively) and the number of new leaves (5.9 and 8.15 for the Hibernal and Johanniter cultivars, respectively). As the CH concentration in the medium increased, the length of shoots and roots decreased. We found the highest percentage of acclimatised plants on WPM (cv. Hibernal) and WPM with 40 ppm of CH (cv. Johanniter). The addition of mT had a negative effect on the morphological traits we measured, regardless of the concentration. Leaves of explants from WPM with mT were yellower and redder compared with the control. None of the explants obtained on this medium produced roots.

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壳聚糖和间topolin对葡萄球菌增殖培养基的补充研究。

研究了壳聚糖(CH)和间topolin (mT)对葡萄(Vitis vinifera cv)体外生长和温室环境适应的影响。约翰尼斯特和cv。冬天的。在萌发阶段，我们将外植体转移到含有浓度为10、20和40 ppm的壳聚糖(分子量3.33 kDa)的木本植物培养基(Woody Plant Media, WPM)上，或者转移到含有浓度为0.5、1.0和2.0 mg - 1的mT的WPM上。不含CH或mT的WPM作为对照。在各试验组合中，ch10 ppm WPM的平均根长和新叶数最大(冬眠和约翰品种分别为12.18 cm和12.65 cm)，新叶数最大(冬眠和约翰品种分别为5.9 cm和8.15 cm)。随着培养基中CH浓度的增加，芽和根的长度减小。我们发现WPM上驯化植物的比例最高(cv。冬眠)和WPM与40 ppm的CH (cv。铀铜矾)。mT的添加对我们测量的形态性状有负影响，无论浓度如何。与对照相比，经mT处理的WPM外植体叶片颜色变黄、变红。在这种培养基上获得的外植体都不产生根。

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来源期刊

Progress on Chemistry and Application of Chitin and its Derivatives MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

1.10

自引率

16.70%

发文量

期刊介绍： Progress in the Chemistry and Application of Chitin and its Derivatives is an annual journal focused on all aspects of production, modification, enzymology and application of chitin and its many derivatives, including chitosan. The journal publishes full-length papers as well as invited reviews. To be considered, papers must present original research that has not been published or accepted for publication elsewhere. The language of the journal will be English.