Chitosan and chitin are promising biopolymers used in many areas including biomedical applications, such as tissue engineering and viscosupplementation. Chitosan shares similar properties with hyaluronan, a natural component of synovial fluid, making it a good candidate for joint disease treatment. The structural and energetic consequences of intermolecular interactions are crucial for understanding the biolubrication phenomenon and other important biomedical features. However, the properties of biopolymers, including their complexation abilities, are influenced by the nature of the aqueous medium with which they interact. In this study, we employed molecular dynamics simulations to describe the effect of pH and the presence of sodium and calcium cations on the stability of molecular complexes formed by collagen type II with chitin and chitosan oligosaccharides. Based on Gibbs free energy of binding, all considered complexes are thermodynamically stable over the entire pH range. The affinity between chitosan oligosaccharide and collagen is highly influenced by pH, while oligomeric chitin shows no pH-dependent effect on the stability of molecular assemblies with collagen. On the other hand, the presence of sodium and calcium cations has a negligible effect on the affinity of chitin and chitosan for collagen.
{"title":"MOLECULAR DYNAMICS SIMULATIONS OF THE AFFINITY OF CHITIN AND CHITOSAN FOR COLLAGEN: THE EFFECT OF pH AND THE PRESENCE OF SODIUM AND CALCIUM CATIONS","authors":"Maciej Przybyłek, Piotr Bełdowski","doi":"10.15259/pcacd.28.013","DOIUrl":"https://doi.org/10.15259/pcacd.28.013","url":null,"abstract":"Chitosan and chitin are promising biopolymers used in many areas including biomedical applications, such as tissue engineering and viscosupplementation. Chitosan shares similar properties with hyaluronan, a natural component of synovial fluid, making it a good candidate for joint disease treatment. The structural and energetic consequences of intermolecular interactions are crucial for understanding the biolubrication phenomenon and other important biomedical features. However, the properties of biopolymers, including their complexation abilities, are influenced by the nature of the aqueous medium with which they interact. In this study, we employed molecular dynamics simulations to describe the effect of pH and the presence of sodium and calcium cations on the stability of molecular complexes formed by collagen type II with chitin and chitosan oligosaccharides. Based on Gibbs free energy of binding, all considered complexes are thermodynamically stable over the entire pH range. The affinity between chitosan oligosaccharide and collagen is highly influenced by pH, while oligomeric chitin shows no pH-dependent effect on the stability of molecular assemblies with collagen. On the other hand, the presence of sodium and calcium cations has a negligible effect on the affinity of chitin and chitosan for collagen.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anatoly Sklyar, Oksana Kalinkevich, Aleksei Kalinkevich, Vadym Chivanov
We created composite materials by using a straightforward method, combining chitosan, iodine, and bismuth. We used two chitosan with two different molecular masses. We used iodine in the composites as chitosan iodide and bismuth in the form of tetraiodobismuthanuide. We analysed the resulting materials with scanning electron microscopy, X-ray diffraction, temperature-programmed desorption mass spectrometry, and visible light spectroscopy. We observed that bismuth and iodine were evenly distributed throughout the composites and did not separate into distinct phases. These composite materials have the ability to form organised porous sponges, making them suitable for a wide range of applications in different fields.
{"title":"PREPARATION AND PHYSICOCHEMICAL CHARACTERISTICS OF AN IODINE AND BISMUTH CONTAINING COMPOSITE MATERIAL BASED ON CHITOSAN","authors":"Anatoly Sklyar, Oksana Kalinkevich, Aleksei Kalinkevich, Vadym Chivanov","doi":"10.15259/pcacd.28.015","DOIUrl":"https://doi.org/10.15259/pcacd.28.015","url":null,"abstract":"We created composite materials by using a straightforward method, combining chitosan, iodine, and bismuth. We used two chitosan with two different molecular masses. We used iodine in the composites as chitosan iodide and bismuth in the form of tetraiodobismuthanuide. We analysed the resulting materials with scanning electron microscopy, X-ray diffraction, temperature-programmed desorption mass spectrometry, and visible light spectroscopy. We observed that bismuth and iodine were evenly distributed throughout the composites and did not separate into distinct phases. These composite materials have the ability to form organised porous sponges, making them suitable for a wide range of applications in different fields.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We explored the multiple uses of chitosan in the form of beads and beech sawdust immobilised in chitosan to remove anionic (Reactive Yellow 85 and Reactive Blue 5) and cationic (Basic Violet 10 and Basic Green 4) dyes. For both anionic dyes, with the next adsorption/desorption cycle, the amount of dye accumulated in the adsorbent increased, which resulted in a decrease in the desorption efficiency. The number of possible adsorption/desorption cycles was regulated by the occupation of all active sites. We observed the opposite trend for the cationic dyes: the charge accumulated in the adsorbent after both adsorption and desorption decreased with subsequent cycles. Despite the low accumulated charge compared with the total adsorption capacity of the adsorbents, it was impossible to further adsorb and desorb the dye. The results demonstrated that for both adsorbents tested, it was possible to carry out 6–7 adsorption/desorption cycles, but the desorption efficiency decreased with each cycle.
{"title":"REUSABILITY OF CHITOSAN AND SAWDUST-MODIFIED CHITOSAN FOR ADSORPTION/DESORPTION OF ANIONIC AND CATIONIC DYES","authors":"Urszula Filipkowska, Tomasz Jóźwiak","doi":"10.15259/pcacd.28.002","DOIUrl":"https://doi.org/10.15259/pcacd.28.002","url":null,"abstract":"We explored the multiple uses of chitosan in the form of beads and beech sawdust immobilised in chitosan to remove anionic (Reactive Yellow 85 and Reactive Blue 5) and cationic (Basic Violet 10 and Basic Green 4) dyes. For both anionic dyes, with the next adsorption/desorption cycle, the amount of dye accumulated in the adsorbent increased, which resulted in a decrease in the desorption efficiency. The number of possible adsorption/desorption cycles was regulated by the occupation of all active sites. We observed the opposite trend for the cationic dyes: the charge accumulated in the adsorbent after both adsorption and desorption decreased with subsequent cycles. Despite the low accumulated charge compared with the total adsorption capacity of the adsorbents, it was impossible to further adsorb and desorb the dye. The results demonstrated that for both adsorbents tested, it was possible to carry out 6–7 adsorption/desorption cycles, but the desorption efficiency decreased with each cycle.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present work, we successfully used electrophoretic deposition to produce a new composite chitosan-copper(II) [Cu(II]) ion coating on grade 4 titanium (Ti). We prepared the coating with an environmentally friendly in situ chemical reduction process from a 2% (v/v) aqueous acetic acid solution with 1 g dm–3 chitosan and 10 g dm–3 copper acetate at 20 V for 300 s at room temperature. We evaluated the relationship between physicochemical properties and corrosion resistance of the coatings with energy dispersive spectroscopy, Fourier-transform infrared spectroscopy, a scanning Kelvin probe, and electrochemical methods. The composite coating contained 2.1 ± 0.1 wt.% of Cu, which ensures antibacterial activity. We determined the mechanism and kinetics of the electrochemical corrosion of the composite chitosan–Cu(II) ion coating on grade 4 Ti in saline solution (0.9% sodium chloride) at 37°C. The surface modification makes grade 4 Ti a more attractive material for use in dentistry.
在本工作中，我们成功地利用电泳沉积技术在4级钛(Ti)上制备了一种新型复合壳聚糖-铜(II) [Cu(II])离子涂层。以2% (v/v)的醋酸水溶液为原料，加入1 g dm-3壳聚糖和10 g dm-3醋酸铜，在20 v下室温下反应300 s，采用环境友好的原位化学还原工艺制备了涂层。我们用能量色散光谱、傅里叶变换红外光谱、扫描开尔文探针和电化学方法评估了涂层的物理化学性质与耐蚀性之间的关系。复合镀层含铜量为2.1±0.1 wt.%，具有良好的抗菌活性。研究了壳聚糖- cu (II)复合镀层在37℃盐溶液(0.9%氯化钠)中电化学腐蚀4级Ti的机理和动力学。表面改性使4级钛成为牙科中更有吸引力的材料。
{"title":"THE EFFECT OF A COMPOSITE CHITOSAN/COPPER(II) ION COATING ON THE CORROSION RESISTANCE OF GRADE 4 TITANIUM IN SALINE: PRELIMINARY RESULTS","authors":"Bożena Łosiewicz, Patrycja Osak, Julian Kubisztal","doi":"10.15259/pcacd.28.008","DOIUrl":"https://doi.org/10.15259/pcacd.28.008","url":null,"abstract":"In the present work, we successfully used electrophoretic deposition to produce a new composite chitosan-copper(II) [Cu(II]) ion coating on grade 4 titanium (Ti). We prepared the coating with an environmentally friendly in situ chemical reduction process from a 2% (v/v) aqueous acetic acid solution with 1 g dm–3 chitosan and 10 g dm–3 copper acetate at 20 V for 300 s at room temperature. We evaluated the relationship between physicochemical properties and corrosion resistance of the coatings with energy dispersive spectroscopy, Fourier-transform infrared spectroscopy, a scanning Kelvin probe, and electrochemical methods. The composite coating contained 2.1 ± 0.1 wt.% of Cu, which ensures antibacterial activity. We determined the mechanism and kinetics of the electrochemical corrosion of the composite chitosan–Cu(II) ion coating on grade 4 Ti in saline solution (0.9% sodium chloride) at 37°C. The surface modification makes grade 4 Ti a more attractive material for use in dentistry.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The troublesome symptoms of gastro-oesophageal reflux disease are a serious health, medical, and social problem for patients. The main problem is gastro-oesophageal acid reflux. In this study, I investigated hydrogels that could prevent the destruction of the oesophageal mucosa. I investigated the effect of chitosan and poloxamer 407 on the protective properties of gels. The addition of chitosan to all the tested gels increased their pH and dynamic viscosity. Preparations containing 25% poloxamer 407 showed the highest pH. Texture tests showed the effect of the poloxamer concentration on the adhesion performance of the tested gels. The findings suggest that the gels can be used to treat advanced acid reflux.
{"title":"INCREASING THE EFFECTIVENESS OF CHITOSAN GELS TO PROTECT THE OESOPHAGEAL MUCOSA","authors":"Katarzyna Małolepsza-Jarmołowska","doi":"10.15259/pcacd.28.010","DOIUrl":"https://doi.org/10.15259/pcacd.28.010","url":null,"abstract":"The troublesome symptoms of gastro-oesophageal reflux disease are a serious health, medical, and social problem for patients. The main problem is gastro-oesophageal acid reflux. In this study, I investigated hydrogels that could prevent the destruction of the oesophageal mucosa. I investigated the effect of chitosan and poloxamer 407 on the protective properties of gels. The addition of chitosan to all the tested gels increased their pH and dynamic viscosity. Preparations containing 25% poloxamer 407 showed the highest pH. Texture tests showed the effect of the poloxamer concentration on the adhesion performance of the tested gels. The findings suggest that the gels can be used to treat advanced acid reflux.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patrycja Kurowska, Tomasz Jóźwiak, Urszula Filipkowska, Tadeusz Bakuła
We analysed the feasibility of using unmodified chitin exoskeletons and moults of mealworms (Tenebrio molitor) as adsorbents to remove cationic dyes – Basic Violet 10 (BV10) and Basic Red 46 (BR46) – from aqueous solutions. We evaluated the characteristics of the adsorption materials using Fourier-transform infrared spectroscopy and determining the pH of the point of zero charge (pHPZC); the pH effect on dye adsorption effectiveness; dye adsorption kinetics (pseudo–first order, pseudo–second order, and intramolecular diffus ion models); and the maximum adsorption capacity of the adsorbents (Langmuir 1 and 2 and Freundlich isotherms). BV10 adsorption on the tested adsorbents was the highest at pH 3, while adsorption of BR46 was highest at pH 6. The adsorption equilibrium time depended mainly on the dye type and its initial concentration; it was 150–210 min for BV10 and 120–150 min for BR46. The maximum adsorption capacity of mealworm exoskeletons reached 5.56 mg/g for BV10 and 31.53 mg/g for BR46, whereas mealworm moults exhibited a higher maximum adsorption capacity, reaching 6.44 mg/g for BV10 and 5.56 mg/g for BR46.
{"title":"THE USE OF CHITIN IN THE MOULTS AND EXOSKELETONS OF MEALWORMS (TENEBRIO MOLITOR) TO REMOVE CATIONIC DYES FROM AQUEOUS SOLUTIONS","authors":"Patrycja Kurowska, Tomasz Jóźwiak, Urszula Filipkowska, Tadeusz Bakuła","doi":"10.15259/pcacd.28.006","DOIUrl":"https://doi.org/10.15259/pcacd.28.006","url":null,"abstract":"We analysed the feasibility of using unmodified chitin exoskeletons and moults of mealworms (Tenebrio molitor) as adsorbents to remove cationic dyes – Basic Violet 10 (BV10) and Basic Red 46 (BR46) – from aqueous solutions. We evaluated the characteristics of the adsorption materials using Fourier-transform infrared spectroscopy and determining the pH of the point of zero charge (pHPZC); the pH effect on dye adsorption effectiveness; dye adsorption kinetics (pseudo–first order, pseudo–second order, and intramolecular diffus ion models); and the maximum adsorption capacity of the adsorbents (Langmuir 1 and 2 and Freundlich isotherms). BV10 adsorption on the tested adsorbents was the highest at pH 3, while adsorption of BR46 was highest at pH 6. The adsorption equilibrium time depended mainly on the dye type and its initial concentration; it was 150–210 min for BV10 and 120–150 min for BR46. The maximum adsorption capacity of mealworm exoskeletons reached 5.56 mg/g for BV10 and 31.53 mg/g for BR46, whereas mealworm moults exhibited a higher maximum adsorption capacity, reaching 6.44 mg/g for BV10 and 5.56 mg/g for BR46.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study evaluated hydrogels containing Celugel, 3% hop cone extract obtained from Humulus lupulus L. using supercritical carbon dioxide extraction substrate, and 1% and 2% chitosan for dermatological application to treat inflammatory skin conditions. The presence of chitosan significantly affected the rheological properties of the formulations, including their dynamic viscosity, hardness, consistency, cohesiveness, and blurring time. The formulations containing 2% chitosan showed the best application possibilities. The formulations were evaluated for dissolution of cohumulone, which is an analogue of humulone contained in hop cone extract. The concentration of chitosan in the formulations had a significant effect on the dissolution testing parameters of the active ingredient: as the chitosan concentration increased, the desired effect of prolonged release time of the active ingredient was achieved while maintaining the membrane-forming properties of Celugel.
{"title":"THE EFFECT OF CHITOSAN ON THE PHYSICOCHEMICAL PROPERTIES OF DERMATOLOGICAL PREPARATIONS CONTAINING HUMULUS LUPULUS L. EXTRACT BASED ON CELUGEL","authors":"Bożena Grimling","doi":"10.15259/pcacd.28.003","DOIUrl":"https://doi.org/10.15259/pcacd.28.003","url":null,"abstract":"This study evaluated hydrogels containing Celugel, 3% hop cone extract obtained from Humulus lupulus L. using supercritical carbon dioxide extraction substrate, and 1% and 2% chitosan for dermatological application to treat inflammatory skin conditions. The presence of chitosan significantly affected the rheological properties of the formulations, including their dynamic viscosity, hardness, consistency, cohesiveness, and blurring time. The formulations containing 2% chitosan showed the best application possibilities. The formulations were evaluated for dissolution of cohumulone, which is an analogue of humulone contained in hop cone extract. The concentration of chitosan in the formulations had a significant effect on the dissolution testing parameters of the active ingredient: as the chitosan concentration increased, the desired effect of prolonged release time of the active ingredient was achieved while maintaining the membrane-forming properties of Celugel.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noira Rakhimovna Vokhidova, Shuhrat Shamsiddinovich Khudoyberdiyev, Ilnar Nakipovich Nurgaliev, Sayyora Sharafovna Rashidova
We obtained binary polyelectrolyte complexes containing chitosan from Bombyx mori and collagen with a mass ratio of 10:1; 10:2, and 10:3. During the interaction between the macromolecules, due to the compensation of the positive charges of chitosan with the negative charges of collagen, the zeta potential of the solutions of polyelectrolyte complexes changed from +15.2 to +5.67 mV. We revealed the dependence of the size characteristics of the particles of polyelectrolyte complexes on time and the ratio of macromolecules. An examination of the morphology of the films of polyelectrolyte complexes demonstrated that in the evaluated chitosan/collagen mass ratios, non-spherical nanosized particles up to ≤ 60 nm form on the surface of the films. We evaluated the mechanism of formation of complexes by using Fourier-transform infrared spectroscopy and confirmed the findings with density functional theory and molecular dynamics. We found that the particle diameter is inversely proportional to the diffusion coefficient. The results show that the particles are almost uniformly distributed over the surface of the polymer matrix and have a unimodal character. We obtained reproducible results when using a chitosan/collagen mass ratio of 10:2 to dye natural silk. We found that the complexes contribute to increase the intensity and stability of colour relative to soap and friction.
{"title":"ON OBTAINING BINARY POLYELECTROLYTE COMPLEXES OF CHITOSAN BOMBYX MORI WITH COLLAGEN","authors":"Noira Rakhimovna Vokhidova, Shuhrat Shamsiddinovich Khudoyberdiyev, Ilnar Nakipovich Nurgaliev, Sayyora Sharafovna Rashidova","doi":"10.15259/pcacd.28.017","DOIUrl":"https://doi.org/10.15259/pcacd.28.017","url":null,"abstract":"We obtained binary polyelectrolyte complexes containing chitosan from Bombyx mori and collagen with a mass ratio of 10:1; 10:2, and 10:3. During the interaction between the macromolecules, due to the compensation of the positive charges of chitosan with the negative charges of collagen, the zeta potential of the solutions of polyelectrolyte complexes changed from +15.2 to +5.67 mV. We revealed the dependence of the size characteristics of the particles of polyelectrolyte complexes on time and the ratio of macromolecules. An examination of the morphology of the films of polyelectrolyte complexes demonstrated that in the evaluated chitosan/collagen mass ratios, non-spherical nanosized particles up to ≤ 60 nm form on the surface of the films. We evaluated the mechanism of formation of complexes by using Fourier-transform infrared spectroscopy and confirmed the findings with density functional theory and molecular dynamics. We found that the particle diameter is inversely proportional to the diffusion coefficient. The results show that the particles are almost uniformly distributed over the surface of the polymer matrix and have a unimodal character. We obtained reproducible results when using a chitosan/collagen mass ratio of 10:2 to dye natural silk. We found that the complexes contribute to increase the intensity and stability of colour relative to soap and friction.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We evaluated the effects of chitosan of different molecular weights on the quality of Kamchatka berries after harvest and storage. Chitosan with lower molecular weights (from 3 to 21 kDa) positively influenced the soluble solid content after harvest. However, chitosan had no significant effect on titratable acidity after harvest. Regardless of the chitosan molecular weight, the berries contained from 7.4% to 30% more total polyphenols compared with the control (162 mg per 100 g) after harvest. After storage, the total phenolic content decreased slightly and depended on the storage conditions. The L-ascorbic acid content was the highest after harvest and storage after treatment with the highest molecular weight of chitosan (950k kDa). Chitosan 50 kDa positively influenced the firmness and puncture resistance of the berries after harvest (146% of control) and storage (135% of control).
{"title":"THE EFFECTS OF APPLYING CHITOSAN OF DIFFERENT MOLECULAR WEIGHTS ON THE QUALITY OF KAMCHATKA BERRIES (LONICERA CAERULEA L.): PART 2","authors":"Ireneusz Ochmian, Marcelina Krupa-Małkiewicz","doi":"10.15259/pcacd.28.012","DOIUrl":"https://doi.org/10.15259/pcacd.28.012","url":null,"abstract":"We evaluated the effects of chitosan of different molecular weights on the quality of Kamchatka berries after harvest and storage. Chitosan with lower molecular weights (from 3 to 21 kDa) positively influenced the soluble solid content after harvest. However, chitosan had no significant effect on titratable acidity after harvest. Regardless of the chitosan molecular weight, the berries contained from 7.4% to 30% more total polyphenols compared with the control (162 mg per 100 g) after harvest. After storage, the total phenolic content decreased slightly and depended on the storage conditions. The L-ascorbic acid content was the highest after harvest and storage after treatment with the highest molecular weight of chitosan (950k kDa). Chitosan 50 kDa positively influenced the firmness and puncture resistance of the berries after harvest (146% of control) and storage (135% of control).","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We investigated the efficiency of chitosan (CH) and meta-topolin (mT) on in vitro growth and adaptation to greenhouse conditions of Vitis vinifera cv. Johanniter and cv. Hibernal. After the initiation stage, we transferred explants to Woody Plant Media (WPM) with chitosan (molecular weight 3.33 kDa) at a concentration of 10, 20, and 40 ppm or to WPM with mT at a concentration of 0.5, 1.0, and 2.0 mg l–1. WPM without CH or mT served as a control. Among the tested combinations, WPM with 10 ppm of CH led to the maximum mean root length (12.18 cm and 12.65 cm for the Hibernal and Johanniter cultivars, respectively) and the number of new leaves (5.9 and 8.15 for the Hibernal and Johanniter cultivars, respectively). As the CH concentration in the medium increased, the length of shoots and roots decreased. We found the highest percentage of acclimatised plants on WPM (cv. Hibernal) and WPM with 40 ppm of CH (cv. Johanniter). The addition of mT had a negative effect on the morphological traits we measured, regardless of the concentration. Leaves of explants from WPM with mT were yellower and redder compared with the control. None of the explants obtained on this medium produced roots.
{"title":"CHITOSAN AND META-TOPOLIN SUPPLEMENTATION OF THE MULTIPLICATION MEDIA OF VITIS VINIFERA L.","authors":"Marcelina Krupa-Małkiewicz, Ireneusz Ochmian","doi":"10.15259/pcacd.28.005","DOIUrl":"https://doi.org/10.15259/pcacd.28.005","url":null,"abstract":"We investigated the efficiency of chitosan (CH) and meta-topolin (mT) on in vitro growth and adaptation to greenhouse conditions of Vitis vinifera cv. Johanniter and cv. Hibernal. After the initiation stage, we transferred explants to Woody Plant Media (WPM) with chitosan (molecular weight 3.33 kDa) at a concentration of 10, 20, and 40 ppm or to WPM with mT at a concentration of 0.5, 1.0, and 2.0 mg l–1. WPM without CH or mT served as a control. Among the tested combinations, WPM with 10 ppm of CH led to the maximum mean root length (12.18 cm and 12.65 cm for the Hibernal and Johanniter cultivars, respectively) and the number of new leaves (5.9 and 8.15 for the Hibernal and Johanniter cultivars, respectively). As the CH concentration in the medium increased, the length of shoots and roots decreased. We found the highest percentage of acclimatised plants on WPM (cv. Hibernal) and WPM with 40 ppm of CH (cv. Johanniter). The addition of mT had a negative effect on the morphological traits we measured, regardless of the concentration. Leaves of explants from WPM with mT were yellower and redder compared with the control. None of the explants obtained on this medium produced roots.","PeriodicalId":44461,"journal":{"name":"Progress on Chemistry and Application of Chitin and its Derivatives","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135031709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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