Detection and genotyping of Giardia duodenalis infecting pigs and small ruminants in the state of Piauí, northeastern Brazil

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES Pesquisa Veterinaria Brasileira Pub Date : 2023-01-01 DOI:10.1590/1678-5150-pvb-7330

Deiviane A. Calegar, Beatriz Coronato-Nunes, Polyanna A.A. Bacelar, Kerla J.L. Monteiro, Jéssica P. Santos, Brenda B.C. Evangelista, Lauren H. Jaeger, Filipe A. Carvalho-Costa

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Abstract

ABSTRACT: This study performed a molecular detection and characterization of Giardia duodenalis infecting pigs, goats and sheep in rural and peri-urban communities in the state of Piauí, northeastern Brazil, and proposed phylogenetic relationships among the characterized parasites. We assessed 52 fecal samples from pigs, 13 from goats, and 10 from sheep. A fragment of the β-giardin locus was PCR-amplified and sequenced. Overall, PCR-based G. duodenalis positivity was 11/52 (21.2%) in pigs, 2/13 (15.4%) in goats, and 2/10 (20%) in sheep. Seven out of 15 successfully amplified samples could be sequenced: three from pigs, two from goats, and two from sheep. Parasites from different hosts were found to belong to sub-assemblage AII. The phylogenetic analyses of the original G. duodenalis AII β-giardin sequences obtained from distinct host species and sequences of G. duodenalis recovered from humans available in GenBank suggest that the parasites are genetically related, supporting a local scenario of cross-host transmission.

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巴西东北部Piauí州猪和小反刍动物感染十二指肠贾第虫的检测和基因分型

摘要:本研究对巴西东北部Piauí州农村和城郊社区猪、山羊和绵羊感染的十二指肠贾第鞭毛虫进行了分子检测和鉴定，并提出了所鉴定寄生虫之间的系统发育关系。我们评估了52份猪粪便样本，13份山羊粪便样本和10份绵羊粪便样本。用pcr扩增了β-栀子苷位点的片段，并对其进行了测序。总体而言，基于pcr的十二指肠分枝杆菌阳性在猪中为11/52(21.2%)，在山羊中为2/13(15.4%)，在绵羊中为2/10(20%)。15个成功扩增的样本中有7个可以测序:3个来自猪，2个来自山羊，2个来自绵羊。不同寄主的寄生虫均属于AII亚组合。从不同宿主物种获得的十二指肠棘豆绦虫AII β-贾丁素原始序列和GenBank中从人类身上恢复的十二指肠棘豆绦虫序列的系统发育分析表明，这两种寄生虫具有遗传相关性，支持本地跨宿主传播的假设。

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来源期刊

Pesquisa Veterinaria Brasileira 农林科学-兽医学

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

9-18 weeks

期刊介绍： Pesquisa Veterinária Brasileira - Brazilian Journal of Veterinary Research (http://www.pvb.com.br), edited by the Brazilian College of Animal Pathology in partnership with the Brazilian Agricultural Research Organization (Embrapa) and in collaboration with other veterinary scientific associations, publishes original papers on animal diseases and related subjects. Critical review articles should be written in support of original investigation. The editors assume that papers submitted are not being considered for publication in other journals and do not contain material which has already been published. Submitted papers are peer reviewed. The abbreviated title of Pesquisa Veterinária Brasileira is Pesqui. Vet. Bras.