Phytochemical screening, total phenolic content and antioxidant activities of Marrubium vulgare L. collected in three development stages

Q3 Medicine Notulae Scientia Biologicae Pub Date : 2023-09-07 DOI:10.55779/nsb15311615
Aya KHOUCHLAA, Abdessadek RAHIMI, Mostafa MIMOUNI, Chaimae RAIS, Hamid KHAMAR, Fatna ZAAKOUR
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Abstract

The change of phenolic compounds and antioxidant activities of plants extracts are widely attributed to numerous conditions and parameters viz. geographical conditions, type of extraction, and type of extract. Thus, this study was the first one conducted to evaluate the phenolic composition, the content of total polyphenols, flavonoids, and proanthocyanidins, and the antioxidant activities of methanolic extract of M. vulgare aerial part collected in three development stages (before flowering, during flowering, and after flowering periods). Phytochemical screening was carried out using standard methods of precipitation and coloration reactions. The total polyphenol, flavonoid, and proanthocyanidins contents were determined using the Folin-Ciocalteu method, aluminum chloride colorimetric method, and vanillin method, respectively. The antioxidant activity was assessed by two methods: DPPH and ABTS scavenging activity. Preliminarily phytochemical screening revealed the presence of saponins, essential oil, cardiac glucosides, sterols and triterpenes, and the absence of alkaloids. In addition, tannins were absence in March and present in May and July. The total phenol content of M. vulgare extracts showed a highest flavonoid and proanthocyanidin contents during flowering period (1.644 mg QE.g-1 extract, and 0.195 mg CE.g-1 extract, respectively) while polyphenol content was highest before flowering period (0.043 mg GAE.g-1 extract). Afterward, M. vulgare methanolic extract exhibited a potent antioxidant activity during flowering period with an IC50 value of 2.23 mg.ml-1. From the current study, this plant contains natural antioxidant substances which can be used as antioxidant agents to treat several diseases.
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3个发育阶段采集的凡黄的植物化学筛选、总酚含量及抗氧化活性
植物提取物中酚类化合物和抗氧化活性的变化受地理条件、提取类型、提取类型等诸多条件和参数的影响。因此,本研究首次对花前、花中、花后3个发育阶段采集的草地上部甲醇提取物的酚类成分、总多酚、总黄酮、原花青素含量及抗氧化活性进行了评价。采用沉淀和显色反应的标准方法进行植物化学筛选。采用Folin-Ciocalteu法、氯化铝比色法和香兰素法分别测定总多酚、类黄酮和原花青素的含量。通过DPPH和ABTS清除活性两种方法评价其抗氧化活性。初步的植物化学筛选显示,该植物中含有皂苷、精油、心糖苷、甾醇和三萜,不含生物碱。单宁在3月不存在,5月和7月存在。黄酮和原花青素含量在开花期最高(1.644 mg QE)。g-1提取物,0.195 mg CE。花期前多酚含量最高,为0.043 mg。g - 1提取级)。结果表明,花楸甲醇提取物在花期表现出较强的抗氧化活性,IC50值为2.23 mg.ml-1。从目前的研究来看,该植物含有天然抗氧化物质,可作为抗氧化剂治疗多种疾病。
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来源期刊
Notulae Scientia Biologicae
Notulae Scientia Biologicae Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
1.10
自引率
0.00%
发文量
63
审稿时长
12 weeks
期刊最新文献
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