A Brief Review on Hyphenated Techniques

Abstract

The hyphenated techniques are developed by combining two or three different analytical techniques. Mainly chromatographic techniques are combined with spectroscopic techniques. The separated components obtained from chromatographic techniques are then transmitted to spectroscopic detection technique through an interface. The improvements in hyphenated analytical methods in last two decades have significantly broadened their applications in the analysis of biomolecules especially in natural products. In this review article recent advancement in hyphenated techniques such as GC-MS, LC-MS, LC-NMR, MS-MS, LC-NMR-MS have been highlighted. The term ‘Hyphenated’ refers to separation and identification of the compounds. These techniques also show better analysis of sample compounds with better accuracy, precision and specificity. This review mainly focuses on the instrumental aspects of hyphenated techniques along with their interfaces. The interfaces such as Jet\Orifice separator interface, Electrospray interface, Atmospheric pressure chemical ionization interface have been covered. It has been concluded that these hyphenated techniques have become more advantageous for the analysis of drugs. This technique is useful for quality control, analytical research, impurity profiling and maintenance for human welfare and development.