Ovarian rejuvenation by PRP (Platelet –Rich Plasma)

Abstract

: Background: Ovarian rejuvenation is a potential therapeutic approach aimed at enhancing ovarian function and improving fertility results in women with poor ovarian reserve (POR). Aim: To compare PRP and normal saline injections impacts on (FSH-LH-AMH-E2) & AFC in POR patients. Methods: This pilot multi-center study included 20 patients with poor ovarian response (POR). Patients underwent laparoscopy, with Group A receiving an autologous PRP injection and Group B receiving a normal saline injection. Hormone levels, including FSH, LH, estradiol, and AMH, were monitored before and every month after PRP injection for a duration of 6 months. Antral follicles count was also assessed prior and following PRP treatment. Results: According to the ovulation status of Group A after PRP injection, out of the 10 participants, 3 (30%) ovulated, while 7 (70%) did not ovulate. Antral follicular count post treatment showed a significant negative correlation with FSH (p<0.001) and LH (p=0.002). Antral follicular count post treatment showed a significant positive correlation with E2 (p=0.001) while no significant correlation with age and AMH. Conclusions: PRP therapy leads to a substantial decrease in LH and FSH concentrations. Additionally, PRP treatment leads to a substantial elevation in estradiol (E2) levels. Antral follicle count (AFC), a marker of ovarian reserve and follicular growth, significantly increases after PRP therapy, suggesting enhanced follicular development.