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In Vivo synergistic effect of curcumin in combination with marbofloxacin against E coli infection in rats 姜黄素与马勃沙星联用对大鼠大肠杆菌感染的体内协同作用
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.282638.1806
Rania Abdelrazek Mohammed Ahmed, M. Elsayed, Enas Farag, Mohamed Aboubakr
Keywords This study aimed to evaluate the potential synergistic effect of curcumin in combination with marbofloxacin against E coli infection in rats. Twenty-five male rats were segregated equally into five groups of Wister albino rats; Group (1) acted as the control group (normal saline only, 0.2 ml); Group (2) was given E. Coli . Group (3) included rats infected with E. coli, and were administered an oral dose of marbofloxacin (5 mg/kg b.wt) for 5 days; Group (4) also administered the same oral dose of marbofloxacin daily for 5 days. . Group (5) rats were treated to E. coli by a single intraperitoneal injection of 2 ml of (1 × 10 9 ) of colony forming units/ml. Following that, rats were received oral dosages of both marbofloxacin and curcumin (20 mg/kg b.wt) Serum biochemical analysis revealed that in marbofloxacin treated group, albumin levels were significantly (P<0.05) decreased mean while transaminases (ALT and AST), alkaline phosphatase (ALP), total bilirubin, triglycerides, and cholesterol were significantly increased during marbofloxacin treatment. On the other hand, rats treated with marbofloxacin and curcumin showed noticeably decreased levels of triglycerides, cholesterol, and transaminases (ALT and AST), and significantly increased levels of albumin compared to rats that received marbofloxacin only. Histopathological examinations of liver tissue revealed severe liver damage in E.coli infected rats and counteracted by marbofloxacin and more treated through curcumin combination. In conclusion, the administration of curcumin in combination with marbofloxacin had a potential synergistic effect on the liver damage caused by E. coli infection in rats.
关键词 本研究旨在评估姜黄素与马勃沙星联合使用对大肠杆菌感染大鼠的潜在协同作用。将 25 只雄性 Wister 白化大鼠平均分成 5 组:第(1)组为对照组(仅使用生理盐水,0.2 毫升);第(2)组给予大肠杆菌(E. Coli)治疗;第(3)组包括感染大肠杆菌的大鼠。组(3)包括感染大肠杆菌的大鼠,口服 5 毫克/千克体重的马勃沙星,连续 5 天;组(4)每天口服相同剂量的马勃沙星,连续 5 天。第(5)组大鼠腹腔注射 2 毫升(1 × 10 9 )菌落形成单位/毫升的大肠杆菌。血清生化分析表明,马勃沙星治疗组的白蛋白平均水平显著下降(P<0.05),而转氨酶(谷丙转氨酶和谷草转氨酶)、碱性磷酸酶(ALP)、总胆红素、甘油三酯和胆固醇在马勃沙星治疗期间显著升高。另一方面,与只接受马勃沙星治疗的大鼠相比,接受马勃沙星和姜黄素治疗的大鼠的甘油三酯、胆固醇和转氨酶(谷丙转氨酶和谷草转氨酶)水平明显下降,而白蛋白水平则明显上升。肝组织的组织病理学检查显示,大肠杆菌感染的大鼠肝脏损伤严重,而服用马勃沙星可抵消损伤,姜黄素联合疗法的疗效更佳。总之,姜黄素与马勃沙星联合用药对大肠杆菌感染引起的大鼠肝损伤具有潜在的协同作用。
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引用次数: 0
A potential therapeutic impact of Gallic acid in a rat model of hepatocarcinogenesis through inhibition of cell proliferation and oncogenic miRNA-221 and induction of apoptosis by Nrf-2 /Bcl-2/TGF- β1 signaling pathways 没食子酸通过抑制细胞增殖和致癌 miRNA-221 以及通过 Nrf-2 /Bcl-2/TGF- β1 信号通路诱导细胞凋亡,对大鼠肝癌模型具有潜在的治疗作用
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.279991.1802
Aʼlaa El-Sayed Al-Semelawy, Samy Ali Aziza, Hussein Abd El-Maksoud, Y. A. El-Senosi, Afaf Desoky Abel Magid
ABSTRACT
摘要
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引用次数: 0
Molecular Docking analysis of the antibacterial effect of pomegranate (Punica granatum) fruit molasses bioactive compounds 石榴(Punica granatum)果实糖蜜生物活性化合物抗菌效果的分子对接分析
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.284813.1816
Hanaa S. Bekeir, Ahmed Hamad, Nesreen Z. Eleiwa, reham A. Amin
Keywords Pomegranate molasses is a functional element that has generated extensive scientific attention in the search for effective bioactive natural compounds. The in-silico effects of pomegranate molasses on foodborne pathogens were investigated using molecular docking analysis, using the crystal structures of E. coli topoisomerase II DNA gyrase B as a target. On the ATP-active pocket of E. coli DNA gyrase, the binding affinities and interactions of ellagic acid, gallic acid, punicalagin, and punicalin were examined. According to the findings, novobiocin had a docking score of -6.30 Kcal/mol, whereas the bioactive components of pomegranate molasses (punicalagin, punicalin, gallagic acid, and ellagic acid) adopted the best binding style with a score of -7.2 to -9.8 Kcal/mol. Our findings suggested that pomegranate molasses could provide powerful antibacterial agents and DNA gyrase inhibitors via unique structural properties that address antimicrobial resistance. They are being investigated further as a natural biocontrol agent. Future research to investigate the actual effects of pomegranate fruit molasses against E. coli using both plate inhibition assays and different
关键词 石榴糖蜜是一种功能性元素,在寻找有效的生物活性天然化合物方面引起了科学界的广泛关注。本文以大肠杆菌拓扑异构酶 II DNA 回旋酶 B 的晶体结构为研究对象,通过分子对接分析研究了石榴糖蜜对食源性致病菌的作用。在大肠杆菌 DNA 回旋酶的 ATP 活性袋上,研究了鞣花酸、没食子酸、番泻苷和番泻甙的结合亲和力和相互作用。研究结果表明,新生物素的对接得分为-6.30 Kcal/mol,而石榴糖浆中的生物活性成分(punicalagin、punicalin、没食子酸和鞣花酸)采用了最佳的结合方式,得分为-7.2至-9.8 Kcal/mol。我们的研究结果表明,石榴糖浆具有独特的结构特性,可提供强效抗菌剂和 DNA 回旋酶抑制剂,从而解决抗菌药耐药性问题。目前正在对石榴糖蜜作为天然生物控制剂进行进一步研究。未来的研究将使用平板抑制试验和不同的方法来调查石榴果糖蜜对大肠杆菌的实际效果。
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引用次数: 0
A field investigation of the impact of the interaction between IBR vaccination and insemination time in cow herd: Reproductive and economic perspectives 对牛群中 IBR 疫苗接种与授精时间之间相互作用影响的实地调查:繁殖和经济角度
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.293231.1830
Wesam Yousef Kamel, Amira M. Abd-El Hamed, Ehab ElNahas, Mahmoud E.A. AbouElRoos, G. Sosa, Mohamed M. M. Kandiel
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引用次数: 0
Biofilm formation and regulating genes of Salmonella enterica recovered from chicken and fish samples. 从鸡肉和鱼肉样本中提取的肠炎沙门氏菌的生物膜形成和调控基因。
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.287159.1821
Ahmed Hamad, Faten S. Hassanin, A. Salem, reham A. Amin
Keywords The study sought to evaluate the biofilm formation capacity and biofilm-related gene profiles of Salmonella enterica recovered from food of animal origins. A total of 24 Salmonella enterica strains were isolated from 215 samples collected from food of animal origins (Raw chicken breast (45 samples), breaded chicken product (45 samples), shrimp (25 samples), Mackerel fish (25 samples), sea bass fillet (25 samples), tilapia fillet (25 samples), and mugil fillet (25 samples)) in Al Qalyubia Governorate, Egypt, during 2020– 2022. Using the crystal violet quantitative microtiter plate method and Congo red agar plating, the isolates' ability to form biofilms was evaluated. Polymerase Chain Reaction analysis was used to identify the presence of four biofilm-related genes ( csgA, csgD, sdiA, and sipA ) in strains that were highly capable of forming biofilms. The results revealed that 62.50% (15/24) of the strains exhibited strong biofilm formation, 20.83% (5/24) were moderate biofilm producers, and 16.67 % (4/24) were weak biofilm producers. Over 80% of the Salmonella enterica strains that are known to produce strong biofilm were found to be positive for all four of the biofilm-related genes when the strains were analyzed. The current study findings underscored the importance of developing effective preventive and control techniques to address the issues posed by biofilm formation in the food industry.
关键词 该研究旨在评估从动物源性食品中回收的肠炎沙门氏菌的生物膜形成能力和生物膜相关基因图谱。研究人员从 2020-2022 年期间在埃及卡留比亚省采集的 215 份动物源性食品(生鸡胸肉(45 份)、裹粉鸡肉(45 份)、虾(25 份)、鲭鱼(25 份)、鲈鱼片(25 份)、罗非鱼片(25 份)和鲻鱼片(25 份))样品中分离出 24 株肠炎沙门氏菌。采用水晶紫定量微孔板法和刚果红琼脂平板法评估了分离物形成生物膜的能力。聚合酶链式反应分析被用来鉴定极易形成生物膜的菌株中是否存在四种生物膜相关基因(csgA、csgD、sdiA 和 sipA)。结果显示,62.50% 的菌株(15/24 株)具有较强的生物膜形成能力,20.83% 的菌株(5/24 株)具有中等的生物膜生成能力,16.67% 的菌株(4/24 株)具有较弱的生物膜生成能力。在对已知会产生强生物膜的肠炎沙门氏菌菌株进行分析时,发现超过 80% 的菌株的四种生物膜相关基因均呈阳性。目前的研究结果强调了开发有效的预防和控制技术以解决食品工业中生物膜形成问题的重要性。
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引用次数: 0
Determination of fish quality in Alexandria small-scale fisheries: A value chain analysis 确定亚历山大小型渔业的鱼类质量:价值链分析
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.260284.1772
sally Ebrahem Abdelaziz, Mai Salah, Haiam M. Aboul-Ela, Reda Fahim
Keywords The Egyptian Mediterranean fisheries sector is a critical contributor to the country's economy, with fish exports accounting to approximately 40% of the total agricultural exports. This sector also plays a vital role in providing employment opportunities, particularly for those living along the Mediterranean coast. However, the sector has been facing various challenges that threaten its sustainability, including overfishing, illegal fishing practices, and environmental degradation. A value chain analysis can help to identify the various stages of the fisheries sector and their interdependencies. In this study, four value chain stages (vessels, wholesalers, retailers, and hypermarkets) were conducted in Alexandria, Egypt, for particular 30 fish species ( Mullus barbatus, Sardinella aurita, and Diplodussargus ). To conduct the value chain study in the sampling areas of small-scale fisheries, the following parameters were assessed: total volatile nitrogen (TVN), thiobarbituric acid (TBA), and pH, The results of the TBA tests and TVN analysis revealed notable disparities between the stages. These tests were frequently used to estimate the quality and freshness of fish, and their findings showed that samples with higher concentrations of these substances had more failures. Conclusively, the current study recommended stricter regulations on harvest size restrictions, monitoring programs, certification procedures, postharvest facilities, seasonal closures, social enterprises, finance facilities, and habitat protection as suggested by applying value chain analysis in small-scale fisheries management.
关键词 埃及地中海渔业部门对国家经济做出了重要贡献,水产品出口约占农业出口总额的 40%。该行业在提供就业机会方面也发挥着至关重要的作用,尤其是为生活在地中海沿岸的人们提供就业机会。然而,该行业一直面临着威胁其可持续性的各种挑战,包括过度捕捞、非法捕鱼行为和环境退化。价值链分析有助于确定渔业部门的各个阶段及其相互依存关系。在这项研究中,针对埃及亚历山大的 30 种特定鱼类(Mullus barbatus、Sardinella aurita 和 Diplodussargus)进行了四个价值链阶段(渔船、批发商、零售商和大卖场)的分析。为了在小型渔业采样区开展价值链研究,对以下参数进行了评估:总挥发性氮(TVN)、硫代巴比妥酸(TBA)和 pH 值。这些检测通常用于评估鱼的质量和新鲜度,其结果表明,这些物质浓度较高的样本不合格的情况较多。最后,本研究建议在小型渔业管理中应用价值链分析法,在捕捞规模限制、监测计划、认证程序、捕捞后设施、季节性休渔、社会企业、融资设施和栖息地保护等方面制定更严格的法规。
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引用次数: 0
Prevalence and critical antibiotic-resistance traits of Bacillus cereus and Staphylococcus aureus isolated from raw and ready-to-eat meat products 从生肉制品和即食肉制品中分离出的蜡样芽孢杆菌和金黄色葡萄球菌的流行率和关键抗生素耐药性特征
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.283334.1809
Eman Masood, F. Mohamed, Nahla Abo EL-Roos, I. Sabike
Keywords The study aimed to compare Bacillus cereus ( B. cereus ) and Staphylococcus aureus (S . aureus ) prevalence and antimicrobial resistance profiles in raw and ready-to-eat meat products (RTEM). One hundred samples of raw hamburger, sausage, RTEM hawawshi, and kofta were tested using standard culture and automated VITEK2 methods. PCR was used to detect resistance genetic components. The overall prevalence of S. aureus and B. cereus was 20%, and 14%, respectively. Staphylococcus aureus and B. cereus were isolated at similarly high rates (60.0% and 73.7%, respectively) from raw meat products. Five percent (1/20) of the B . cereus isolates detected in raw burger samples were multidrug-resistant (MDR), whereas 25 % of S . aureus were MDR, with three resistance patterns. Neither the mcr 1 nor the van A genes were found in the B . cereus . While nor A was found in four isolates, both bla TEM and bla CTX were found in three. The bla SHV was found only in three raw-derived isolates, two of which also shared bla TEM , bla CTX , and nor A or bla TEM and nor A. The data revealed that all of the MDR S . aureus isolates tested positive for mec A but not van A genes. Such pathogens in RTE meat with genes confer resistance to key antibiotics, endangering public health and hastening the emergence of superbugs.
关键词 该研究旨在比较生肉和即食肉制品(RTEM)中蜡样芽孢杆菌(B. cereus)和金黄色葡萄球菌(S. aureus)的流行率和抗菌药耐药性概况。使用标准培养和自动 VITEK2 方法检测了 100 个生汉堡包、香肠、RTEM Hawawshi 和 Kofta 样品。利用 PCR 检测抗药性基因成分。金黄色葡萄球菌和蜡样芽孢杆菌的总体流行率分别为 20% 和 14%。金黄色葡萄球菌和蜡样芽孢杆菌在生肉制品中的分离率同样很高(分别为 60.0% 和 73.7%)。在生汉堡样本中检测到的蜡样芽孢杆菌中,5%(1/20)具有多重耐药性(MDR),而 25% 的金黄色葡萄球菌具有多重耐药性,有三种耐药性模式。在蜡样芽孢杆菌中既没有发现 mcr 1 基因,也没有发现 van A 基因。在 4 个分离株中发现了 nor A,在 3 个分离株中发现了 bla TEM 和 bla CTX。数据显示,所有 MDR 金黄色葡萄球菌分离物的 mec A 基因检测均呈阳性,但 van A 基因检测不呈阳性。带有这些基因的即食肉类中的病原体会对主要抗生素产生耐药性,从而危及公众健康并加速超级细菌的出现。
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引用次数: 0
Chemical profile of Salted and Smoked Fish 咸鱼和熏鱼的化学成分概况
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.238557.1729
Yomna Soliman, Walid Arab, A. Edris, Amina Elrais
ABSTRACT
摘要
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引用次数: 0
Molecular characterization of isolated duck viral hepatitis and its pathogenicity in one day and seven day old ducklings in Qalyubia governorate. 卡留比亚省一天龄和七天龄雏鸭分离出的鸭病毒性肝炎的分子特征及其致病性。
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.279397.1800
Aya Hamdy Behery, K. Zyan, Ahmed Eissa Saad, A. El-Shemy
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引用次数: 0
The effect of a 3D-modeled pyramidal packing shape on the growth of foodborne pathogens inoculated in ribeye-lion at dynamic room temperatures. 三维建模的金字塔填料形状对动态室温下接种到肋眼石狮中的食源性病原体生长的影响。
Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.270643.1789
Samaa Yehya, Shimaa N. Edris, Ahmed Hamad, Islam I. Sabeq
Keywords Food packaging serves several important tasks, including preserving and controlling foodborne pathogens. One component that contributes significantly to these functions is the design of the food packaging itself. Bacterial retardation of natural meat biota was demonstrated using pyramidal packing patterns designed by the Great Pyramid's dimension ratios at constant chilling temperatures. The current study, based on the Giza-pyramid dimension ratio, used dynamic room temperature to evaluate the consequences of pyramidal packaging on minced ribeye meat experimentally contaminated with foodborne pathogens: Listeria monocytogenes and Salmonella Typhimurium , as well as naturally occurred Escherichia coli . The pyramidal growth curves were compared to cuboidal and cylindrical 3D-printed containers. The APC count increased marginally in all three forms with time, but declined considerably in pyramidal stored beef at 8, 14, 18, and 24 hours and circular stored beef at 32, 48 hours (P < 0.05). The result revealed that the packaging shape had no significant effect on the growth curves of both S. Typhimurium and E. coli . The pyramidal packaging negatively impacted the L. monocytogens growth curve, which was more obvious at the end of the storage period than other packaging shapes. The current study was conducted at dynamic room temperature, which may counteract pyramidal effect noticed when storage occurred at constant temperature. Also, the delayed or non-significant impact of the pyramidal package observed here on growth curves of the inoculated pathogen, particularly the Gram-negative one, could be attributable to other factors such as type of packaging material magnetic field stability. Conclusively, the hierarchical shape had optimal and efficient effects on the control of microbial growth introduced in minced meat therefore, it essential to consider incorporate the findings of these studies when developing packaging options that efficiently preserve food.
关键词 食品包装具有多项重要功能,包括保存和控制食源性病原体。食品包装本身的设计是实现这些功能的重要因素之一。根据大金字塔的尺寸比设计的金字塔包装模式在恒定的冷藏温度下证明了天然肉类生物群的细菌阻滞作用。目前的研究以吉萨金字塔的尺寸比为基础,利用动态室温评估了金字塔包装对受食源性病原体污染的肉糜的影响:这些病原体包括单核细胞增生李斯特菌和鼠伤寒沙门氏菌,以及自然发生的大肠杆菌。金字塔形生长曲线与立方体和圆柱形 3D 打印容器进行了比较。随着时间的推移,所有三种形式的 APC 数量都略有增加,但在 8、14、18 和 24 小时内,金字塔形储存牛肉的 APC 数量显著下降,在 32 和 48 小时内,圆形储存牛肉的 APC 数量显著下降(P < 0.05)。结果表明,包装形状对伤寒杆菌和大肠杆菌的生长曲线没有显著影响。金字塔形包装对单核细胞增多症杆菌的生长曲线有负面影响,与其他形状的包装相比,这种影响在储存期结束时更为明显。目前的研究是在动态室温下进行的,这可能会抵消恒温储存时的金字塔效应。此外,这里观察到的金字塔形包装对接种病原体(尤其是革兰氏阴性病原体)生长曲线的延迟或无显著影响可能是由其他因素造成的,如包装材料类型的磁场稳定性。因此,在开发有效保存食品的包装方案时,必须考虑纳入这些研究结果。
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引用次数: 0
期刊
Benha Veterinary Medical Journal
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