Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in Rhodotorula mucilaginosa

IF 2.1 Q3 MICROBIOLOGY Microbiology Research Pub Date : 2023-09-18 DOI:10.3390/microbiolres14030096
Joseph Antony Sundarsingh Tensingh, Vijayalakshmi Shankar
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Abstract

The overconsumption of energy results in the depletion of fossil fuels. Generally, biodiesels are produced from wastes of animal fats and vegetable oils. In this study, we have tried to produce biodiesel from both the wild strain and ion beam mutated strain and compared the concentration of lipids produced from both the strains and their properties. Lipids were extracted from microbes using the Bligh and Dyer method and analyzed using gas chromatography and mass spectrophotometry (GCMS) and Fourier-transform infrared (FTIR) spectroscopy. Extracted lipids (free fatty acids) were converted into biodiesel (fatty acid methyl esters) using a base catalyst. The end product biodiesel was characterized and analyzed based on ASTM standards.
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诱导黏胶红霉菌氮离子突变提高野生菌株脂质产量提高生物柴油产量
能源的过度消耗导致了化石燃料的枯竭。一般来说,生物柴油是从动物脂肪和植物油的废料中生产出来的。在这项研究中,我们尝试用野生菌株和离子束突变菌株生产生物柴油,并比较了两种菌株生产的脂质浓度和它们的性质。采用Bligh和Dyer法从微生物中提取脂质,并采用气相色谱和质谱法(GCMS)和傅里叶变换红外光谱法(FTIR)进行分析。提取的脂质(游离脂肪酸)使用碱催化剂转化为生物柴油(脂肪酸甲酯)。根据ASTM标准对最终产物生物柴油进行了表征和分析。
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来源期刊
Microbiology Research
Microbiology Research MICROBIOLOGY-
CiteScore
1.90
自引率
6.70%
发文量
62
审稿时长
10 weeks
期刊介绍: Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.
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