Phytochemical screening and anti-hyperglycemic effect of Jatropha multifida L. ethanol extract and its fraction on a high-fat diet and Streptozotocin-induced diabetic rats

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Abstract

Jatropha multifida L. is a plant traditionally recognized for its medicinal properties. This plant has been reported to possess anti-microbial, anti-oxidative, wound healing and anti-inflammatory properties. The study aimed to evaluate the anti-diabetic potentials of J. multifida. The study deals with the extraction of the leaves of the plant by using 50% ethanol followed by fractionation of the ethanol extract obtained. Various physicochemical parameters and phytochemical analyses were done by TLC and HPTLC. Phytochemical analysis of the extract and its fraction indicated high concentrations of flavonoids, phenolic acid and saponins. The effects of the 50% ethanol extract of J. multifida leaves, and its hexane fraction were investigated in high-fat diet and Streptozotocin-induced diabetic rats. At the end of the treatment, the reduction in blood glucose level in rats treated with ethanol extract at a dose of 400 mg/kg was found to be 117.5±1.7 and hexane fraction at 200 mg/kg was found to be 105±2.5. A significant improvement in lipid profile and liver abnormalities of the rats was observed. The result of the present study is also justified by histopathological examinations of the pancreas and liver. Therefore, the current study proved that both the ethanolic extract of J. multifida and its hexane fraction possess bioactive constituents that could be responsible for the anti-diabetic properties. Out of the two, maximum improvement was seen using hexane fraction at 200 mg/kg. This may be due to the active phytoconstituents isolated from the fraction of the 50% ethanolic extract of the leaves.
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麻疯树乙醇提取物及其组分对高脂肪饮食和链脲佐菌素诱导的糖尿病大鼠的植物化学筛选及降糖作用
麻疯树是传统上公认的药用植物。据报道,这种植物具有抗微生物、抗氧化、伤口愈合和抗炎的特性。本研究旨在评价多裂参的抗糖尿病潜能。该研究处理了用50%乙醇提取植物叶片,然后对得到的乙醇提取物进行分馏。采用薄层色谱法和高效液相色谱法对其理化参数进行分析。植物化学分析表明,提取物及其组分含有高浓度的黄酮类化合物、酚酸和皂苷。研究了多裂叶50%乙醇提取物及其己烷部分对高脂肪饮食和链脲佐菌素诱导的糖尿病大鼠的影响。治疗结束时,400 mg/kg乙醇提取物对大鼠血糖水平的降低为117.5±1.7,200 mg/kg己烷提取物对大鼠血糖水平的降低为105±2.5。观察到大鼠的脂质谱和肝脏异常有明显改善。本研究的结果也被胰腺和肝脏的组织病理学检查所证实。因此,本研究证明了多花莲乙醇提取物及其己烷部分均具有可能具有抗糖尿病作用的生物活性成分。其中,使用200 mg/kg的己烷馏分效果最好。这可能是由于从叶子的50%乙醇提取物的部分中分离出的活性植物成分。
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来源期刊
CiteScore
1.00
自引率
12.50%
发文量
1
审稿时长
52 weeks
期刊介绍: Indian Journal of Natural Products and Resources (IJNPR), a quarterly journal, is dedicated to the exploration of topics that confront both educators, frontline workers in the fields, entrepreneurs and ultimate users of the value added products and their resources pertaining to both plants and animals. It publishes original research papers, reviews and short communications. The scope of the journal is multidisciplinary especially of applied nature. Papers on plant cultivation, fishery, apiary, poultry, dairy, disease and pest control measures, postharvest technology/practices, value added products and utilization of plants and animals in broader sense (including ethnobotany and pharmacology) are considered for publication.
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