Study of Pharmacological Profile of Chloroform Leaf Extract of Ludwigia perennis - A Wetland Plant

Abstract

Aim: The main objective of this study was to find out various therapeutic properties of chloroform leaf extract of the wetland plant Ludwigia perennis. Place and Duration of Study: Department of Biotechnology, Sree Narayana Guru College, Coimbatore, Tamilnadu, Scigen Research and Innovation in Thanjavur, Tamil Nadu; and the Sri Lakshmi Narayana Institute of Medical Sciences in Osudu, Pondicherry between April 2023 and August 2023. Methodology: Chloroform leaf extract of Ludwigia perennis was employed for evaluating the antioxidant, antidiabetic, anti-nutritional, anticancer, anti-inflammatory, and antipyretic properties, respectively. Results: The DPPH assay found that the IC50 for the leaf extract was 48.78µg/mL, while the IC50 for the standard was 49.74µg/mL. The IC50 for the phosphomolybdenum test was 32.31µg/mL for the leaf extract and 33.73µg/mL for the standard. The IC50 values for the hydroxyl radical scavenging experiment were 55.6µg/mL for the standard and 47.54µg/mL for the leaf extract. The IC50 of the standard in the α-amylase inhibitory assay is 394.48µg/mL, and the extract's IC50 is 330.38µg/mL, respectively. The IC50 of the standard in the α-glucosidase inhibitory assay was 394.06µg/mL, and the extract's IC50 was 335.45µg/mL, respectively. Antinutrient content such as alkaloids was 21.4%, oxalate was 6.6%, phytate was 4.18%, and saponin was 30%, respectively. The IC50 of HeLa cells treated with leaf extract was 180µg/mL. After 4hours of treatment with chloroform leaf extract, the inhibition was 51.75% when 50mg/kg was used and 58.74% when 250mg/kg was used. Here, the inhibitory percentage of indomethacin is 59.45%. This means that the leaf extract has anti-inflammatory activity. Chloroform leaf extracts produced significant antipyretic effects in 250 and 500mg/kg doses. Conclusion: Therefore, the chloroform leaf extract of Ludwigia perennis has shown to have anti-oxidant, anti-diabetic, anti-nutrient, anti-cancer, anti-inflammatory, and anti-pyretic activities. These evidences suggest the plant is a potential source for developing new drugs.