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Cultivation of Vetiver (Chrysopogon zizanioides (L.) Roberty)- A Versatile Medicinal and Aromatic Plant 香根草(Chrysopogon zizanioides (L.) Roberty)的栽培--一种多用途药用和芳香植物
Pub Date : 2024-07-25 DOI: 10.9734/ejmp/2024/v35i51199
Yashaswini Sharma
Vetiver (Chrysopogon zizanioides) is a versatile medicinal and aromatic plant mainly used for soil and conservation purposes. It is a perennial grass species with a densely tufted enormous root system that grows up to two meters long. The fibrous roots are highly aromatic and used for extraction of essential oils used in high-grade perfumes as a base or fixative for their long-lasting characteristic odor. The fragrant dry roots are traditionally used for water purification in south India. Besides, the consumption of vetiver-treated water has cooling properties and refreshes the body and mind. It also acts as a deodorant and stimulant, aids digestion, and has carminative, colic, anthelmintic, and antioxidant properties. It is a very hard grass, suitable for growing in wastelands, arid regions, and hill slopes with the least maintenance. The worldwide demand for vetiver oil was estimated at around 408.8 t/year in 2019, and the growth was reported to increase at a CAGR of 7.8 % from 2020-2027. India consumes 100 tons of vetiver oil annually; the domestic production is only 20 tons, and the remaining 80 % of the oil is imported. Hence, there is a lot of scope for increasing the vetiver area in India and globally. Looking at its vast utilization and demand, an attempt has been made to present the improved cultivation practices of vetiver based on the literature survey and experience. 
香根草(Chrysopogon zizanioides)是一种用途广泛的药用和芳香植物,主要用于土壤和水土保持。它是一种多年生禾本科植物,具有密集丛生的巨大根系,可长达两米。它的须根芳香浓郁,可用于萃取精油,作为高级香水的基质或固定剂,气味持久。在印度南部,芳香的干根传统上用于净化水。此外,饮用香根草处理过的水有降温的作用,能让人身心清爽。它还具有除臭和刺激作用,能帮助消化,并具有催吐、止痛、驱虫和抗氧化的功效。它是一种非常坚硬的草,适合生长在荒地、干旱地区和山坡上,维护成本最低。据估计,2019 年全球香根草油需求量约为 408.8 吨/年,2020-2027 年的年复合增长率为 7.8%。印度每年消耗 100 吨香根草油,国内产量仅为 20 吨,其余 80% 的香根草油需要进口。因此,印度和全球都有很大的空间来增加香根草的种植面积。鉴于香根草的广泛利用和需求,我们尝试根据文献调查和经验介绍香根草的改良种植方法。
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引用次数: 0
Influence of Certain Biostimulants on the Vegetative Growth of Annual Moringa (Moringa oleifera. Lam) 某些生物刺激剂对一年生辣木植物生长的影响
Pub Date : 2024-07-23 DOI: 10.9734/ejmp/2024/v35i51198
Huria, F. M, E. H. Shaaban, M. K. Gaber
Due to the importance of moringa leaves which can help improve the dietary diversity and quality of households that need to improve their nutritional intake, being a potential alternate source of protein, and for the sake of increasing its growth and yield, this study is determine the effect of some biofertilizers, amino acids and seaweed extract on vegetative growth of moringa  seedlings As well as, to find out the best fertilization treatment to improve the vegetative growth of Moringa and reducing the intensive use of chemical fertilizers. This study was conducted in the Experimental Farm of the Faculty of Agriculture (Saba-Basha), Alexandria University, Egypt, during two consecutive seasons of 2020 and 2021, to study the effect of biofertilizers, amino acids, and seaweed extract and its combinations on the growth of Moringa (Moringa oleifera, L.). The experiment was designed as split-split plot design it included 27 treatments with three replicates. The results revealed that the highest significant values of vegetative growth as plant height (171.8 cm), stem length (87.30 cm), stem diameter (3.49 cm), leaves fresh weight (70.61 g), leaves dry weight (13.35g), number of leaves per plant (72.03), and number of leaves per shoot (54.41) were achieved when the trees were soil bio-fertilized and sprayed with the investigated amino acid 700 mg/l + seaweed extract 700 mg/l + cerealine (T26) for both seasons of study. While the number of branches per plant (15.28), shoot fresh weight (138.1g), and shoot dry weight (24.07g) were concerned, the highest values were obtained when the plants were treated with soil bio-fertilization (Phosphorine), sprayed with amino acids, and seaweed extract, as the abovementioned treatments, amino acid 700 mg/l + seaweed extract 700 mg/l + Phosphorine (T25), for both seasons of study compared to the other treatments and control.
由于辣木叶的重要性,它可以帮助改善需要提高营养摄入的家庭的膳食多样性和质量,是一种潜在的替代蛋白质来源,为了提高其生长和产量,本研究确定了一些生物肥料、氨基酸和海藻提取物对辣木幼苗无性生长的影响,并找出最佳施肥处理方法,以改善辣木的无性生长,减少化肥的大量使用。本研究于 2020 年和 2021 年连续两季在埃及亚历山大大学农学院(萨巴-巴沙)实验农场进行,旨在研究生物肥料、氨基酸和海藻提取物及其组合对辣木(Moringa oleifera, L.)生长的影响。实验采用分小区设计,包括 27 个处理,三个重复。结果表明,在研究的两个季节里,对树木进行土壤生物施肥并喷洒所研究的氨基酸 700 毫克/升+海藻提取物 700 毫克/升+谷维素(T26)时,植物生长的最高显著值为株高(171.8 厘米)、茎长(87.30 厘米)、茎直径(3.49 厘米)、叶片鲜重(70.61 克)、叶片干重(13.35 克)、每株叶片数(72.03)和每枝叶片数(54.41)。在每株分枝数(15.28)、嫩枝鲜重(138.1 克)和嫩枝干重(24.07 克)方面,与其他处理和对照相比,在研究的两个季节中,使用土壤生物施肥(磷)、喷洒氨基酸和海藻提取物(如上述处理,氨基酸 700 毫克/升+海藻提取物 700 毫克/升+磷(T25))处理的植株获得的数值最高。
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引用次数: 0
Potential Biological and Antimicrobian Effects of the Essential Oil of Aniba rosaeodora: A Review of the Literature Aniba rosaeodora 精油的潜在生物和抗菌作用:文献综述
Pub Date : 2024-06-14 DOI: 10.9734/ejmp/2024/v35i41193
Thaynara Monteiro Paiva Garcia, Mateus Silva Tavares, Renan Miranda Correa, Anthony Matheus dos Santos Gomes, Luana Nóbrega dos Santos, Anderson Lima Bentes, Rita de Cássia Silva de Oliveira
Aims: Map the literature in search of the main biological and antimicrobial effects of the essential oil (EO) of Aniba rosaeodora. Study Design: This is an integrative review based on the PRISMA method. Place and Duration of Study: Center for Biological and Health Sciences of a University in the Brazilian Amazon, from January to August 2022. Methodology: A search was carried out in the main databases, such as Embase, Scopus, PubChem, PubMed, LILACS, SciELO and Portal BVS, with the descriptors consulted in the Medical Subject Headings (MeSH). Results: 134 articles were found and, after applying the inclusion and exclusion criteria, 17 were selected for full analysis. The EO of Aniba roseadora revealed mainly antibacterial, antifungal, antiparasitic and antiviral properties and in two studies, anesthetic effects, without observation of serious adverse events and deaths, were observed, but the specific active compound was not identified. The antibacterial activity of linalool, the compound most present in Aniba roseadora EO, was significant. Aniba roseadora EO also showed inhibitory and fungicidal potential. In addition, Aniba roseadora had an antidepressant effect, reducing anhedonia. Conclusion: The EO of Aniba rosaeadora showed potential biopharmacological and microbiological activities in pre-clinical models. Linalool stood out as the substance with the highest concentration in the EO; however, it is not yet known whether this compound is the main active component. Therefore, more studies should be conducted to support and describe the pharmacological potential of Aniba rosaeadora EO, leading to evidence-based pharmacology.
研究目的:查阅文献,探究玫瑰木精油(EO)的主要生物和抗菌作用。研究设计:这是一项基于 PRISMA 方法的综合综述。研究地点和时间:巴西亚马逊地区一所大学的生物与健康科学中心,2022 年 1 月至 8 月。研究方法:在 Embase、Scopus、PubChem、PubMed、LILACS、SciELO 和 Portal BVS 等主要数据库中进行检索,并参考医学主题词表(MeSH)中的描述符。结果:共找到 134 篇文章,在应用纳入和排除标准后,选出 17 篇进行全面分析。Aniba roseadora 的环氧乙烷主要具有抗菌、抗真菌、抗寄生虫和抗病毒的特性,在两项研究中,观察到了麻醉效果,但未观察到严重的不良反应和死亡病例,但未确定具体的活性化合物。芳樟醇是 Aniba roseadora 环氧乙烷中含量最高的化合物,具有显著的抗菌活性。Aniba roseadora 环氧乙烷还具有抑制和杀真菌的潜力。此外,Aniba roseadora 还具有抗抑郁作用,可减轻失神症状。结论Aniba rosaeadora 的环氧乙烷在临床前模型中显示出潜在的生物药理和微生物活性。芳樟醇是环氧乙烷中浓度最高的物质,但这种化合物是否是主要的活性成分还不得而知。因此,应开展更多的研究来支持和描述 Aniba rosaeadora 环氧乙烷的药理潜力,从而形成以证据为基础的药理学。
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引用次数: 0
Nutrient, Phytonutrient Composition, and Antioxidant Activity Analysis of Fresh and Cabinet Dried Coconut (Cocos nucifera L.) Inflorescence 新鲜椰子(Cocos nucifera L.)花序和柜干椰子(Cocos nucifera L.)花序的营养成分、植物营养成分和抗氧化活性分析
Pub Date : 2024-06-04 DOI: 10.9734/ejmp/2024/v35i41192
Nikhila.P. Vinod, Jancy Rani D
Background: Lately manufactories with medicinal properties play an important part in food and medicinal diligence for their capacities on disease prevention and treatment. Phytonutrients and antioxidant are the naturally occurring compounds found in the plant materials which has positive effects to avoid the oxidative stress-related diseases like cancer, cardiovascular disease, and diabetes. Antioxidant protects cells from the damage caused by the free radicals and to slow down or prevent oxidation of the other molecules. Antioxidants are extremely important in many plant base foods. Coconut (Cocos nucifera L.) inflorescence are rich in Phytonutrients and antioxidants. Nutrient are compound in the food which provide energy that facilitates repair, growth and also helps to carry out different life processes. They can help in reducing the risk of diseases and improves overall health. Hence for the objectives of the present study is to determine the nutrient composition and to estimate the phytonutrients and to analyse the antioxidant activity of fresh and the cabinet dry (45℃for 24h) Cocos nucifera (L.) inflorescence. Methods and Results: Nutrients such as carbohydrate (0.23g,0.19g), protein (24.7g,21.5g), fibre (11%,34.3%), iron(0.115mg,0.135), vitamin-C (0.01mcg,0.0062mcg), calcium (2.8mg,1.6mg) were analyzed by the AOAC method. The Preliminary qualitative phytonutrient analysis was carried out by the standard methodology with extraction of the plant material through the maceration process to identify the secondary metabolites like alkaloids, flavonoids, quinons, phenol, saponin, tannin, terpenoids and steroids in various solvents of aqueous, ethanol, methanol, acetone, petroleum ether and chloroform. Antioxidant activity were analyzed by the DPPH method. Conclusion: Cabinet dried sample have a better profile of the phytonutrients and antioxidant activity compared to fresh sample. So it is better to utilize cabinet dried sample in product development and supplementation of Cocos nucifera (L.) inflorescence.
背景:近来,具有药用价值的植物因其在预防和治疗疾病方面的能力而在食品和药品领域发挥着重要作用。植物营养素和抗氧化剂是植物材料中天然存在的化合物,对避免癌症、心血管疾病和糖尿病等与氧化应激有关的疾病有积极作用。抗氧化剂保护细胞免受自由基的损害,减缓或防止其他分子的氧化。抗氧化剂在许多植物性食物中都极为重要。椰子(Cocos nucifera L.)花序富含植物营养素和抗氧化剂。营养素是食物中的化合物,可提供能量,促进修复和生长,还有助于进行不同的生命过程。它们有助于降低疾病风险,改善整体健康。因此,本研究的目的是确定新鲜和柜式干燥(45℃ 24 小时)椰子(L.)花序的营养成分,估算植物营养素,并分析其抗氧化活性。方法和结果:采用 AOAC 方法分析了碳水化合物(0.23 克、0.19 克)、蛋白质(24.7 克、21.5 克)、纤维素(11%、34.3%)、铁(0.115 毫克、0.135)、维生素 C(0.01 毫微克、0.0062 毫微克)、钙(2.8 毫克、1.6 毫克)等营养成分。植物营养素初步定性分析采用标准方法,通过浸泡过程提取植物材料,在各种溶剂(水、乙醇、甲醇、丙酮、石油醚和氯仿)中鉴定生物碱、黄酮类、醌类、酚类、皂苷、单宁、萜类和类固醇等次生代谢物。抗氧化活性采用 DPPH 法进行分析。结论与新鲜样品相比,柜干样品具有更好的植物营养素和抗氧化活性。因此,在椰子花序的产品开发和补充中使用柜式干燥样品更好。
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引用次数: 0
Extraction and Purification of Squalene from Virgin Olive Oil via Catalytic Transesterification and Molecular Distillation 通过催化酯交换反应和分子蒸馏从初榨橄榄油中提取和纯化角鲨烯
Pub Date : 2024-06-03 DOI: 10.9734/ejmp/2024/v35i31191
H. K. Manikyam, Dhanaseelan J, Jayaraj Mani
Squalene, a highly valuable compound abundant in various natural sources, shows great potential in pharmaceutical, cosmetic, and nutraceutical applications. This research article outlines a comprehensive methodology for extracting and purifying squalene from virgin olive oil, a rich source of the compound. The extraction process begins with degumming, which involves heating the olive oil to 60-70°C to reduce viscosity, followed by the addition of 2-3% warm water to hydrate phospholipids. Food-grade phosphoric acid is then added to react with the phospholipids, forming precipitates. The mixture is stirred for 20-30 minutes and allowed to rest for an additional 20-30 minutes, enabling impurities to settle. The upper layer of degummed oil is separated via decantation or centrifugation and washed with warm water for pH adjustment. Next, transesterification is performed by mixing 100 ml of virgin olive oil with 25% methanol (w/w) and a catalyst (0.5% sodium methoxide or PTSA), and heating the mixture to 80-90°C under reflux for 1-2 hours. Following transesterification, the solvent and acetone are distilled out, and acetone precipitation is repeated 2-3 times to remove unsaponified matter, which is then filtered and evaporated. The concentrated oil undergoes molecular distillation at 180°C and 0.0033 bar pressure for 1 hour, yielding the distillate and residue for further analysis. Qualitative analysis using Thin Layer Chromatography (TLC) involves Merck TLC plates with silica gel 60 F254 and a hexane: chloroform (9:1) mobile phase. Spots are developed with a 10% HCl solution, confirming the presence of squalene with an RF value of 0.93. Quantitative analysis via High-Performance Thin-Layer Chromatography (HPTLC) employs Merck TLC plates with cyclohexane as the mobile phase and CAMAG at 254 nm and 366 nm wavelengths, revealing a squalene purity of 67% and a recovery rate of 69.8%. The initial purification through transesterification facilitated the conversion of ester groups, yielding squalene-rich fractions, while acetone precipitation effectively removed saponified matter. Molecular distillation further enhanced squalene purity. TLC analysis confirmed the qualitative presence of squalene, and HPTLC provided precise quantitative measurements. The obtained squalene purity of 67% significantly enriches the initial content in virgin olive oil, though further optimization could enhance purity and yield. Complementary techniques like GC-MS or HPLC could validate the purification process. This study presents an efficient, replicable procedure for extracting and purifying squalene from virgin olive oil, with significant implications for pharmaceutical, cosmetic, and nutraceutical industries. The findings support a sustainable and ethical shift towards vegetable-derived squalene, meeting market demands while ensuring high-quality production.
角鲨烯是一种非常有价值的化合物,在各种天然资源中含量丰富,在医药、化妆品和营养保健品方面具有巨大的应用潜力。本文概述了从角鲨烯含量丰富的初榨橄榄油中提取和纯化角鲨烯的综合方法。提取过程首先是脱胶,将橄榄油加热到 60-70°C 以降低粘度,然后加入 2-3% 的温水使磷脂水合。然后加入食品级磷酸与磷脂反应,形成沉淀物。将混合物搅拌 20-30 分钟,再静置 20-30 分钟,使杂质沉淀。通过倾析或离心分离上层脱胶油,并用温水清洗以调节 pH 值。接下来进行酯交换反应,将 100 毫升初榨橄榄油与 25% 的甲醇(重量比)和催化剂(0.5% 甲醇钠或 PTSA)混合,在回流条件下将混合物加热至 80-90°C 并持续 1-2 小时。酯交换反应后,蒸馏出溶剂和丙酮,丙酮沉淀重复 2-3 次,以除去未皂化物,然后过滤并蒸发。浓缩油在 180°C 和 0.0033 巴压力下进行分子蒸馏 1 小时,得到蒸馏物和残留物,供进一步分析。使用默克公司的 TLC 板(硅胶 60 F254)和正己烷:氯仿(9:1)流动相进行薄层色谱法(TLC)定性分析。用 10% HCl 溶液显色,确认角鲨烯的存在,RF 值为 0.93。通过高效薄层色谱法(HPTLC)进行定量分析,采用默克 TLC 板,以环己烷为流动相,CAMAG 波长为 254 纳米和 366 纳米,结果表明角鲨烯的纯度为 67%,回收率为 69.8%。通过酯交换反应进行的初步纯化促进了酯基的转化,得到了富含角鲨烯的馏分,而丙酮沉淀则有效地去除了皂化物。分子蒸馏进一步提高了角鲨烯的纯度。TLC 分析证实了角鲨烯的定性存在,而 HPTLC 则提供了精确的定量测量。所获得的 67% 角鲨烯纯度大大提高了初榨橄榄油中的初始含量,不过进一步优化还可以提高纯度和产量。GC-MS 或 HPLC 等辅助技术可以验证纯化过程。这项研究提出了一种高效、可复制的从初榨橄榄油中提取和纯化角鲨烯的方法,对制药、化妆品和保健品行业具有重要意义。研究结果支持向植物角鲨烯的可持续和道德转变,在满足市场需求的同时确保高质量生产。
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引用次数: 0
Hypoglycaemic Effects of an Aqueous Leaves Extract of Combretum micranthum in Normoglycaemic Rats 康普顿叶水提取物对血糖正常大鼠的降血糖作用
Pub Date : 2024-05-02 DOI: 10.9734/ejmp/2024/v35i31190
Djike Kouehiouon Justin Anderson-Bel, Zahoui Ouga Stanislas, Ekra Etchien N’dah Christelle, N. S. Anthelme, Flavien Traore
Background: In order to promote the Ivorian pharmacopoeia, we undertook the study of Combretum micranthum (Combretaceae), a plant commonly used in Ivory Coast for the treatment of diabetes. The aim of this study was to evaluate the hypoglycemic effects of an aqueous leaves extract of Combretum micranthum (EaqCM) in Wistar rats. Methods and Materials: A phytochemical screening study was performed. The pharmacological test on normoglycaemic rats was carried out in 4 groups of 5 rats per group. Rats in group 1 received 2 ml of distilled water, and rats in the test groups received doses ranging at 100 to 300 mg/kg B.W. of the EAqCM. Tests in pre-treated temporarily hyperglycemic rats were performed in 4 groups of 5 rats per group. The rats in groups 1 and 2 received 2ml of distilled water, the rats in test groups (3 and 4) received the dose of 10-2g/kg B.W. and 100 mg/kg B.W. respectively glibenclamide and EAqCM. Thirty minutes later, all rats except those in group 1 received anhydrous glucose. The protocol for testing post-treated hyperglycaemic rats is the same as that for pretreated rats, except that in this experiment glucose is administered to the animals before the test substances. Blood glucose is measured in the rats at regular intervals of 30, 60, 90, 120, 150, and 180 minutes after administration of the substances. Results: Qualitative phytochemical analysis of EAqCM revealed the presence of polyphenols, flavonoids, catechic, tannins, quinonic compounds, alkaloids, sterols, and polyterpenes. Pharmacological tests on the glycemia of normoglycemic rats showed that EAqCM has hypoglycemic properties at a dose of 100 mg/kgB.W. with a percentage reduction of 30%. In hyperglycemic rats, EAqCM and glibenclamide exhibited anti-hyperglycemic activity with respective percentages reduction of 57.47 % and 58.82 %. Conclusion: Antihyperglycemic effect of EAqCM in post-treated rats would probably be due to the presence of polyphenols and flavonoids in this extract. These results support the use of this plant in traditional medicine for the treatment of diabetes.
背景:为了促进科特迪瓦药典的发展,我们对象牙海岸常用来治疗糖尿病的植物 Combretum micranthum(康柏科)进行了研究。本研究的目的是评估 Combretum micranthum 的水性叶提取物(EaqCM)对 Wistar 大鼠的降血糖作用。方法和材料:进行植物化学筛选研究。对血糖正常的大鼠进行药理试验,共分 4 组,每组 5 只。第 1 组大鼠接受 2 毫升蒸馏水,试验组大鼠接受 100 至 300 毫克/千克体重的 EAqCM 剂量。对经过预处理的暂时性高血糖大鼠进行的试验分 4 组进行,每组 5 只大鼠。第 1 和第 2 组大鼠接受 2 毫升蒸馏水,试验组(第 3 和第 4 组)大鼠分别接受剂量为 10-2 克/千克体重和 100 毫克/千克体重的格列本脲和 EAqCM。30 分钟后,除第 1 组大鼠外,其他大鼠均接受无水葡萄糖。试验后高血糖大鼠的方案与试验前高血糖大鼠的方案相同,只是在本实验中,先给动物服用葡萄糖,然后再服用试验物质。在给大鼠注射药物后的 30、60、90、120、150 和 180 分钟定期测量血糖。结果对 EAqCM 的植物化学定性分析显示,其中含有多酚、黄酮类、儿茶酸、单宁、醌类化合物、生物碱、甾醇和多萜类化合物。对血糖正常的大鼠进行的药理测试表明,当剂量为 100 毫克/千克体重时,EAqCM 具有降血糖作用,降糖百分比为 30%。对于高血糖大鼠,EAqCM 和格列本脲具有抗高血糖活性,降糖百分比分别为 57.47 % 和 58.82 %。结论EAqCM 对治疗后大鼠的降血糖作用可能是由于这种提取物中含有多酚和类黄酮。这些结果支持在传统医学中使用这种植物治疗糖尿病。
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引用次数: 0
Effects of Ficus capensis Leaves Aqueous Extracts (Lactogenic Medicinal Plant) on NMRI Mice Milk Secretion 薜荔叶水提取物(泌乳药用植物)对 NMRI 小鼠乳汁分泌的影响
Pub Date : 2024-04-25 DOI: 10.9734/ejmp/2024/v35i21184
Ouedraogo Rakiswendé Alexis, T. Basile, Azonbakin Azandégbé Simon, D. Maya, Ouedraogo Rakissida Alfred, Aweh A Bruno, Ouédraogo Aimé Sosthène, Yameogo Noé, Idani Boureima, Kouraogo M Paul, Kabore Jean Luc, Dabire Charlemagne, Ouedraogo Boris Honoré Amadou, Zouré Abdou Azaque, Ouédraogo W. H. Gautier, B. Balé, Sanou/Lamien Assita
Introduction: Ficus capensis is a plant used in traditional medicine to stimulate lactation in women and animals in Africa. However, the effects of their extracts on the mammary gland are poorly documented. The objective of the study was to evaluate the effects of Ficus capensis leaves aqueous extracts on NMRI mice milk secretion.Methodology: This was an experimental animal study using virgin female NMRI mice aged eight to ten weeks. The mice were grouped into for groups of eight mice each. Each group received one of the following products: distilled water, Galactogil™, sulpiride, aqueous extracts (AE) of capensis leaves. Data were analysed and processed using Microsoft Excel 2016 and Stata MP 16 with P ≤ .05 as the significance threshold.Results: Arborescence of the galactophorous ducts was more developed in the sulpiride lot. Galactogil™, and capensis leaves extracts treated groups showed almost equivalent arborescence with a higher tendency than the distilled water. With histological haematin- eosin staining, the ratio of galactophorous ducts containing secretions to total ducts was higher in the groups of Ficus capensis AE treated group than distilled water (P = .0001). Galactogil™, sulpiride and the group of Ficus capensis extracts each had higher levels of beta-casein in mammary tissue and average prolactinemia than distilled water (P < .01). Mammary tissue stained by immunohistochemistry with anti-prolactin receptor antibodies showed more intensely labelled mammary glands in the sulpiride and Ficus capensis extracts groups. There was no statistically significant difference between average progesteronemia among the different groups.Conclusion: F. capensis leaves AE administered to virgin female NMRI mice showed lactogenic and mammogenic effects. The extracts were able to increase the nutritional quality of the milk produced, as evidenced by the increase in protein secretion.
简介薜荔是非洲传统医学中用来刺激妇女和动物泌乳的一种植物。然而,其提取物对乳腺的影响却鲜有记载。本研究的目的是评估榕树叶水提取物对 NMRI 小鼠乳汁分泌的影响:这是一项动物实验研究,使用的是八至十周大的处女雌性 NMRI 小鼠。小鼠被分组,每组八只。每组接受以下产品中的一种:蒸馏水、Galactogil™、舒必利、毛果芸香叶水提取物(AE)。使用 Microsoft Excel 2016 和 Stata MP 16 分析和处理数据,以 P≤.05 为显著性阈值:结果:舒必利地段的半乳孔导管树状化程度更高。经 Galactogil™ 和 capensis 叶提取物处理的各组显示出几乎相同的腋化,但比蒸馏水处理组的腋化倾向更高。通过组织学血红素-伊红染色,含有分泌物的半乳孔导管与总导管的比率在榕树叶提取物处理组中高于蒸馏水处理组(P = .0001)。与蒸馏水相比,Galactogil™、舒必利和薜荔提取物组乳腺组织中的β-酪蛋白水平和平均泌乳素血症水平都更高(P < .01)。用抗催乳素受体抗体进行免疫组化染色的乳腺组织显示,舒必利组和薜荔提取物组的乳腺标记更强。不同组间平均孕酮血症的差异无统计学意义:结论:给处女雌性 NMRI 小鼠服用薜荔叶 AE 具有泌乳和乳腺增生作用。提取物能够提高所产乳汁的营养质量,蛋白质分泌的增加就是证明。
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引用次数: 0
Phytochemical Profiling of Aqueous Methanol Extract of Terminalia bellirica from Bokaro District of Jharkhand, India 印度恰尔肯德邦博卡洛地区的 Terminalia bellirica 水甲醇提取物的植物化学成分分析
Pub Date : 2024-04-12 DOI: 10.9734/ejmp/2024/v35i21183
Navin Kumar Mahato, Kalpana Prasad, Arun Kumar Roy Mahato
Plants are a vital source of medicine for human being from ancient times. Various plant parts are highly medicinal due to the presence of different bioactive compounds and secondary metabolites. Many of these compounds present in medicinal plants are unknown to the scientific community. T. bellirica is distributed in tropical parts of the world and is a known ethnomedicinal plant that is the reservoir of various bioactive compounds. The present investigated the bioactive and secondary metabolite present in the fruits of T. bellirica collected from the Bokaro district and has been analysed using Gas chromatography-Mass spectrometry analysis. The investigation found 55 compounds from the methanol extract of the fruit sample of T. bellirica. Among the recorded compounds, Pyrogallol is the major constituent with 85% area in the chromatogram, followed by 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)with 5% area, and the rest of the compounds are altogether 10% of the total. In conclusion, the present study recorded and highlighted a total of 55 bioactive compounds from the dry fruits and pericarp, which is more than a double of the compounds earlier known from the plant species. Further, among the recorded biochemical compounds, the concentration of Pyrogallol was alone more than 85% of the total compounds found from the methanol extract of the fruit sample of T. bellirica.
植物自古以来就是人类重要的药物来源。由于存在不同的生物活性化合物和次级代谢产物,各种植物部分都具有很高的药用价值。药用植物中的许多化合物都不为科学界所知。T. bellirica 分布在世界热带地区,是一种已知的民族药用植物,富含各种生物活性化合物。本研究调查了从 Bokaro 地区采集的 T. bellirica 果实中的生物活性物质和次级代谢物,并使用气相色谱-质谱分析法进行了分析。调查从 T. bellirica 果实样本的甲醇提取物中发现了 55 种化合物。在记录的化合物中,焦酚是主要成分,在色谱图中占 85% 的面积,其次是 2,3-二氢-3,5-二羟基-6-甲基-4H-吡喃-4-酮(DDMP),占 5% 的面积,其余化合物共占 10%。总之,本研究从干果和果皮中总共记录并强调了 55 种生物活性化合物,是该植物物种早期已知化合物的两倍多。此外,在记录的生化化合物中,仅焦酚的浓度就超过了从 T. bellirica 果实样本甲醇提取物中发现的化合物总量的 85%。
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引用次数: 0
Antiplasmodial Activity and Phytochemical Evaluation of the Stems of Albizia coriaria and Ficus sur 胡枝子和榕树茎的抗疟活性和植物化学评价
Pub Date : 2024-04-02 DOI: 10.9734/ejmp/2024/v35i21179
K. A. Baah, Akwasi Acheampong, I. Amponsah, R. Nketia
Background: Malaria continues to cause havoc on various populations because of the high mortality and economic burden associated with the disease. Progress made in the therapeutics of the disease is threatened by the emerging parasite resistance to currently used first line treatment drugs. This has prompted the search for new, effective, and safe antimalarial agents. The use of traditional medicine in the treatment of various types of diseases including malaria is a regular practice seen with many cultures in Ghana. The stems of Albizia coriaria Welw ex. Oliver and Ficus sur Forssk are such plants used with little evidence about their in vivo efficacy.Aim: This study therefore aimed to assess the in vivo antiplasmodial potential, and the acute toxicity of the hydroethanolic stem extract of Albizia coriaria and Ficus sur.Method: Qualitative phytochemical screening was done on the powdered plant material using standard methods. Acute toxicity was carried out according to OECD guidelines using the Limit test. In vivo antiplasmodial activity of the hydroethanolic extract was assessed using the Peter’s 4-day suppressive and Rane’s curative test.Results: The 70% ethanol extract was safe with the lethal dose above 3000 mg/kg. All the extracts significantly (P < 0.05) suppressed parasitaemia in the Peter’s suppressive and Rane’s curative test with Albizia coriaria producing the highest chemotherapeutic activity of 68.89 and 61.46% in the suppressive and curative test respectively. That of F. sur was less than 50% in both assays. Artesunate reference drug recorded over 80% suppression in the curative test but lesser activity in the suppression assay compared to A. coriaria. Several plant metabolites including terpenoids, flavonoids and coumarins were found in both plant samples.Conclusion: Albizia coriaria and Ficus sur 70% ethanol extract showed considerable antiplasmoidal activity and were found to be non-toxic in acute toxicity study, thus justifying their safe use in the treatment of malaria as suggested by folklore medicine.
背景:由于疟疾造成的高死亡率和经济负担,该疾病继续对不同人群造成严重破坏。由于寄生虫对目前使用的一线治疗药物产生抗药性,疟疾治疗方面取得的进展受到威胁。这促使人们寻找新的、有效和安全的抗疟药物。使用传统药物治疗包括疟疾在内的各类疾病是加纳许多文化中的常规做法。Albizia coriaria Welw ex.目的:因此,本研究旨在评估 Albizia coriaria 和 Ficus sur Forssk 茎的水乙醇提取物的体内抗疟潜力和急性毒性:方法:采用标准方法对粉末状植物材料进行定性植物化学筛选。急性毒性试验根据经合组织(OECD)准则进行,采用极限试验。采用彼得 4 天抑制试验和雷恩治疗试验评估了水乙醇提取物的体内抗疟活性:结果:70%乙醇提取物是安全的,致死剂量超过 3000 毫克/千克。在彼得抑制试验和雷恩治疗试验中,所有提取物都能明显抑制寄生虫血症(P < 0.05),其中,Albizia coriaria 的化疗活性最高,在抑制试验和治疗试验中分别为 68.89% 和 61.46%。在这两项试验中,F. sur 的化疗活性均低于 50%。与 A. coriaria 相比,青蒿琥酯参考药物在治疗试验中的抑制率超过 80%,但在抑制试验中的活性较低。两种植物样本中都发现了多种植物代谢产物,包括萜类化合物、黄酮类化合物和香豆素类化合物:结论:白花蛇舌草和薜荔70%乙醇提取物显示出相当强的抗疟原虫活性,并且在急性毒性研究中发现它们没有毒性,因此民间医学建议将它们安全地用于治疗疟疾。
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引用次数: 0
Studies on Chemical Changes in Burfi Enriched with different Herbs 富含不同草药的 Burfi 化学变化研究
Pub Date : 2024-03-26 DOI: 10.9734/ejmp/2024/v35i11180
Meshram, B.D., Rokade Apeksha, Shaikh Adil
The investigation was carried out to study the chemical changes taking place in burfi by the addition of several herbs and their comparison with the control product. Different herbs, viz., Withania somnifera (Ashwgandha), Asparagus racemosus (Shatavari), and Ocimum sanctum (Tulsi), were incorporated into the blend in the ratio of 5:4:1, respectively, to develop herbal burfi. The combination of herbs was added at levels of 1.00, 1.25, 1.50, and 2.00% by weight of khoa to obtain products designated T1, T2, T3, and T4, respectively. The control product (T0) was prepared in a similar manner but was devoid of herbs. Proximate analysis, including moisture, total solids, fat, lactose, protein, and ash, was conducted. The results show that moisture content decreased significantly (P≤0.05) with increasing herb addition, while total solids increased. Protein content increased with higher herb percentages, likely due to the protein content of the added herbs. Fat and lactose content remained unchanged with herb addition, as herbs do not contribute significantly to these components. Ash content increased with higher herb addition rates, though not significantly (P≥0.05). Overall, the addition of herbs influenced the chemical composition of burfi, impacting moisture, total solids, protein, and ash content with varying degrees of significance.
调查的目的是研究在布尔菲中添加几种草药后发生的化学变化,并将其与对照产品进行比较。在混合液中分别按 5:4:1 的比例加入了不同的草药,即睡莲(Withania somnifera)、天门冬(Asparagus racemosus)和圣乌头(Ocimum sanctum),以制成草药布菲。草药组合的添加量为 Khoa 重量的 1.00%、1.25%、1.50% 和 2.00%,得到的产品分别为 T1、T2、T3 和 T4。对照产品(T0)的制备方法类似,但不含药材。进行了近似分析,包括水分、总固形物、脂肪、乳糖、蛋白质和灰分。结果表明,随着草药添加量的增加,水分含量明显降低(P≤0.05),而总固形物则有所增加。蛋白质含量随着草药比例的增加而增加,这可能是由于添加的草药蛋白质含量增加所致。脂肪和乳糖含量随香草添加量的增加而保持不变,因为香草对这些成分的影响不大。灰分含量随着草药添加量的增加而增加,但不明显(P≥0.05)。总的来说,添加香草会影响布尔菲的化学成分,对水分、总固形物、蛋白质和灰分含量都有不同程度的影响。
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引用次数: 0
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European journal of medicinal plants
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