The Technical System of Rapid Propagation with Photinia frasery Leaves Inducing Calli, First Generation Culture, Increasing Reproduction, Rooting and Training Seedlings to Transplant

Abstract

The explants of Photinia frasery with leaves were first generation cultured. And then, the calli which were induced by explants were cultured and proliferated, followed by the effective seedlings which were proliferation-targeted cultured, rooting-targeted cultured. Finally, the seedlings are trained and transplanted. The study results showed: (1) Primary culture of Photinia frasery leaves, the best plant hormone combination in medium is: MS+0.4 mg · L −1 BA+1.6 mg · L −1 NAA; (2) The result of orthogonal test, proliferation culture of Photinia frasery , the best plant hormone combination in medium is: MS+2.0 mg·L −1 BA+0.1 mg·L −1 IBA+2.0 mg·L −1 GA+40 g sucrose; (3) Rooting culture of Photinia frasery , the best plant hormone combination in medium is: 1/2MS+0.4 mg·L −1 IBA. The results showed that different impacts of plant hormone type and concentration combination on the primary culture, reproducing culture and rooting culture of Photinia frasery leaves were obvious.