Extraction, Characterization, Antioxidant and Hypoglycemic of Pectic Polysaccharides from Cantaloupe (Cucumis melo L.) Peels

Hui Sun, Ruizhan Chen, Fanlei Meng, Helong Bai, Li Tian, Juan Lu, Chunlong Bai, Dongxue Li, Wenjing Wu, Yongtang Wang, Mingze Gong
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Abstract

Abstract In this study, microwave extraction (ME) of pectic polysaccharides (M‐CPPs) from cantaloupe peels (CPs) is optimized using response surface methodology (RSM) with a Box–Behnken design (BBD). The optimum ME parameters are liquid–solid ratio 26.04 mL g −1 , microwave power 543.04 W, irradiation time 6.38 min, and pH 1.5. Compared heat reflux extraction polysaccharides (H‐CPPs), the yield, the contents of neutral sugars (TSC), uronic acids (UAC), total flavonoids (TFC), total phenolics (TPC), sulfate groups (SGC) of M‐CPPs increased by 156.06%, 15.33%, 7.27%, 1019.05%, 1.75%, and 57.89%, but the molecular weight (Mw) and degree of esterification (DE) reduced by 29.08% and 24.81%, respectively. M‐CPPs exhibit superior antioxidant and hypoglycemic activities than H‐CPPs, which may be attributed to its higher UAC, TFC and SGC, lower Mw, and DE. There is almost no change in the monosaccharide types of isolated polysaccharides, only a change in molar ratio. Results proved that ME is an efficient technique for the extraction and modification of pectic polysaccharides (CPPs) from CPs with high yield, strong antioxidant, and hypoglycemic activities for applications in medical and food industries.

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哈密瓜果胶多糖的提取、表征、抗氧化及降糖作用皮
摘要本研究采用Box-Behnken设计(BBD)优化响应面法(RSM)对哈密瓜皮(CPs)中果胶多糖(M‐CPPs)的微波提取工艺。最佳ME参数为液固比26.04 mL g−1,微波功率543.04 W,辐照时间6.38 min, pH 1.5。与热回流提取的多糖(H‐CPPs)相比,M‐CPPs的产率、中性糖(TSC)、醛酸(UAC)、总黄酮(TFC)、总酚(TPC)、硫酸盐基(SGC)含量分别提高了156.06%、15.33%、7.27%、1019.05%、1.75%和57.89%,而分子量(Mw)和酯化度(DE)分别降低了29.08%和24.81%。M‐CPPs表现出比H‐CPPs更强的抗氧化和降糖活性,这可能是由于其更高的UAC、TFC和SGC,更低的Mw和DE。分离多糖的单糖类型几乎没有变化,只有摩尔比的变化。结果表明,ME是一种高效的从石蜡中提取和改性果胶多糖的技术,具有较高的产率、较强的抗氧化和降糖活性,可用于医药和食品工业。
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